دورية أكاديمية
An in vivo assay of the mutagenic potential of imidacloprid using sperm head abnormality test and dominant lethal test.
| العنوان: | An in vivo assay of the mutagenic potential of imidacloprid using sperm head abnormality test and dominant lethal test. |
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| المؤلفون: | Bagri P; Department of Veterinary Pharmacology and Toxicology and., Kumar V, Sikka AK |
| المصدر: | Drug and chemical toxicology [Drug Chem Toxicol] 2015; Vol. 38 (3), pp. 342-8. Date of Electronic Publication: 2014 Oct 15. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Taylor & Francis Group Country of Publication: United States NLM ID: 7801723 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1525-6014 (Electronic) Linking ISSN: 01480545 NLM ISO Abbreviation: Drug Chem Toxicol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: [Philadelphia, PA] : Taylor & Francis Group Original Publication: New York, Dekker. |
| مواضيع طبية MeSH: | Fertility/*drug effects , Imidazoles/*toxicity , Insecticides/*toxicity , Mutagens/*toxicity , Mutation/*drug effects , Nitro Compounds/*toxicity , Sperm Head/*drug effects , Spermatogonia/*drug effects, Animals ; Embryo Implantation/drug effects ; Embryo Loss/chemically induced ; Embryo Loss/genetics ; Female ; Fertility/genetics ; Male ; Mice ; Mutagenicity Tests ; Neonicotinoids ; Pregnancy ; Risk Assessment ; Sperm Head/pathology ; Spermatogonia/pathology ; Time Factors |
| مستخلص: | Objective: To assess the mutagenic effects of imidacloprid in germ cells of Swiss albino male mice by sperm head abnormality (SHA) assay and dominant lethal test (DLT). Methods: Swiss albino mice were exposed to imidacloprid (22, 11 and 5.5 mg/kg/day) along with 3% gum acacia as vehicle control through oral route for 7, 14 and 28 days for SHA assay and for 28 days for DLT. The epididymal sperm smear in 1% eosin stain was analyzed for SHAs. In DLT, male mice were allowed to mate with females after 1, 3 and 6 weeks of end of pesticide treatment. The uterine contents of the sacrificed females were observed for live and dead implants. The analysis of test and control groups data was done by one way ANOVA at p < 0.05. Results: Exposure of all dose levels of imidacloprid (22, 11 and 5.5 mg/kg/day) for seven days did not induce significant SHAs while they induced significant SHAs compared with the control group following exposure for 14 and 28 days. The analysis of uterine content revealed a significant increase in the number of dead implants/female compared with the vehicle control in only those females which were mated with male mice after six weeks of treatment of highest dose level of imidacloprid. The dominant lethal mutations were observed only at spermatogonial stage. Conclusions: Long-term exposure of pesticide generated SHAs even at lowest dose level (5.5 mg/kg/day for 14 days) and mutagenic effects at spermatogonial stage at highest dose level (22 mg/kg/day for 28 days). |
| فهرسة مساهمة: | Keywords: Dominant lethal test; Swiss albino mice; imidacloprid; mutagenicity; oral route; sperm head abnormalities |
| المشرفين على المادة: | 0 (Imidazoles) 0 (Insecticides) 0 (Mutagens) 0 (Neonicotinoids) 0 (Nitro Compounds) 3BN7M937V8 (imidacloprid) |
| تواريخ الأحداث: | Date Created: 20141016 Date Completed: 20160524 Latest Revision: 20190116 |
| رمز التحديث: | 20240628 |
| DOI: | 10.3109/01480545.2014.966832 |
| PMID: | 25317479 |
| قاعدة البيانات: | MEDLINE |
PDF full text from Publisher | تدمد: | 1525-6014 |
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| DOI: | 10.3109/01480545.2014.966832 |