دورية أكاديمية
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The acetyl bromide method is faster, simpler and presents best recovery of lignin in different herbaceous tissues than Klason and thioglycolic acid methods.

التفاصيل البيبلوغرافية
العنوان: The acetyl bromide method is faster, simpler and presents best recovery of lignin in different herbaceous tissues than Klason and thioglycolic acid methods.
المؤلفون: Moreira-Vilar FC; Department of Agronomy, State University of Maringá, Maringá, Paraná, Brazil., Siqueira-Soares Rde C; Department of Biochemistry, State University of Maringá, Maringá, Paraná, Brazil., Finger-Teixeira A; Department of Biochemistry, State University of Maringá, Maringá, Paraná, Brazil., de Oliveira DM; Department of Biochemistry, State University of Maringá, Maringá, Paraná, Brazil., Ferro AP; Department of Biochemistry, State University of Maringá, Maringá, Paraná, Brazil., da Rocha GJ; Brazilian Bioethanol Science and Technology Laboratory, Campinas, São Paulo, Brazil., Ferrarese Mde L; Department of Biochemistry, State University of Maringá, Maringá, Paraná, Brazil., dos Santos WD; Department of Biochemistry, State University of Maringá, Maringá, Paraná, Brazil., Ferrarese-Filho O; Department of Biochemistry, State University of Maringá, Maringá, Paraná, Brazil.
المصدر: PloS one [PLoS One] 2014 Oct 16; Vol. 9 (10), pp. e110000. Date of Electronic Publication: 2014 Oct 16 (Print Publication: 2014).
نوع المنشور: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Acetates/*chemistry , Chemical Fractionation/*methods , Lignin/*analysis , Lignin/*isolation & purification , Saccharum/*cytology , Glycine max/*cytology , Thioglycolates/*chemistry, Furaldehyde/analogs & derivatives ; Furaldehyde/analysis ; Mechanical Phenomena ; Saccharum/chemistry ; Glycine max/chemistry ; Time Factors
مستخلص: We compared the amount of lignin as determined by the three most traditional methods for lignin measurement in three tissues (sugarcane bagasse, soybean roots and soybean seed coat) contrasting for lignin amount and composition. Although all methods presented high reproducibility, major inconsistencies among them were found. The amount of lignin determined by thioglycolic acid method was severely lower than that provided by the other methods (up to 95%) in all tissues analyzed. Klason method was quite similar to acetyl bromide in tissues containing higher amounts of lignin, but presented lower recovery of lignin in the less lignified tissue. To investigate the causes of the inconsistencies observed, we determined the monomer composition of all plant materials, but found no correlation. We found that the low recovery of lignin presented by the thioglycolic acid method were due losses of lignin in the residues disposed throughout the procedures. The production of furfurals by acetyl bromide method does not explain the differences observed. The acetyl bromide method is the simplest and fastest among the methods evaluated presenting similar or best recovery of lignin in all the tissues assessed.
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المشرفين على المادة: 0 (Acetates)
0 (Thioglycolates)
70ETD81LF0 (5-hydroxymethylfurfural)
7857H94KHM (2-mercaptoacetate)
9005-53-2 (Lignin)
DJ1HGI319P (Furaldehyde)
O18V5XYO0G (acetyl bromide)
تواريخ الأحداث: Date Created: 20141021 Date Completed: 20150622 Latest Revision: 20240314
رمز التحديث: 20240314
مُعرف محوري في PubMed: PMC4212577
DOI: 10.1371/journal.pone.0110000
PMID: 25330077
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0110000