دورية أكاديمية
أعرض في EDS

Staphylococcus aureus ST398 gene expression profiling during ex vivo colonization of porcine nasal epithelium.

التفاصيل البيبلوغرافية
العنوان: Staphylococcus aureus ST398 gene expression profiling during ex vivo colonization of porcine nasal epithelium.
المؤلفون: Tulinski P, Duim B; Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands. b.duim@uu.nl., Wittink FR, Jonker MJ, Breit TM, van Putten JP, Wagenaar JA, Fluit AC
المصدر: BMC genomics [BMC Genomics] 2014 Oct 20; Vol. 15, pp. 915. Date of Electronic Publication: 2014 Oct 20.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 100965258 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2164 (Electronic) Linking ISSN: 14712164 NLM ISO Abbreviation: BMC Genomics Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, [2000-
مواضيع طبية MeSH: Bacterial Proteins/*genetics , Methicillin-Resistant Staphylococcus aureus/*genetics , Nasal Mucosa/*microbiology , Staphylococcal Infections/*veterinary , Swine Diseases/*microbiology, Animals ; Gene Expression Profiling ; Gene Expression Regulation, Bacterial ; In Vitro Techniques ; Methicillin-Resistant Staphylococcus aureus/pathogenicity ; Molecular Sequence Data ; Nasal Mucosa/cytology ; Sequence Analysis, RNA ; Staphylococcal Infections/microbiology ; Swine ; Virulence
مستخلص: Background: Staphylococcus aureus is a common human and animal opportunistic pathogen. In humans nasal carriage of S. aureus is a risk factor for various infections. Methicillin-resistant S. aureus ST398 is highly prevalent in pigs in Europe and North America. The mechanism of successful pig colonization by MRSA ST398 is poorly understood. Previously, we developed a nasal colonization model of porcine nasal mucosa explants to identify molecular traits involved in nasal MRSA colonization of pigs.
Results: We report the analysis of changes in the transcription of MRSA ST398 strain S0462 during colonization on the explant epithelium. Major regulated genes were encoding metabolic processes and regulation of these genes may represent metabolic adaptation to nasal mucosa explants. Colonization was not accompanied by significant changes in transcripts of the main virulence associated genes or known human colonization factors. Here, we documented regulation of two genes which have potential influence on S. aureus colonization; cysteine extracellular proteinase (scpA) and von Willebrand factor-binding protein (vWbp, encoded on SaPIbov5). Colonization with isogenic-deletion strains (Δvwbp and ΔscpA) did not alter the ex vivo nasal S. aureus colonization compared to wild type.
Conclusions: Our results suggest that nasal colonization with MRSA ST398 is a complex event that is accompanied with changes in bacterial gene expression regulation and metabolic adaptation.
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سلسلة جزيئية: GEO GSE47910
المشرفين على المادة: 0 (Bacterial Proteins)
تواريخ الأحداث: Date Created: 20141022 Date Completed: 20150702 Latest Revision: 20211021
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC4210494
DOI: 10.1186/1471-2164-15-915
PMID: 25331735
قاعدة البيانات: MEDLINE
الوصف
تدمد:1471-2164
DOI:10.1186/1471-2164-15-915