دورية أكاديمية
أعرض في EDS

Lattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution.

التفاصيل البيبلوغرافية
العنوان: Lattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution.
المؤلفون: Chen BC; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA., Legant WR; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA., Wang K; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA., Shao L; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA., Milkie DE; Coleman Technologies, Incorporated, Newtown Square, PA 19073, USA., Davidson MW; National High Magnetic Field Laboratory and Department of Biological Science, Florida State University, Tallahassee, FL 32310, USA., Janetopoulos C; Department of Biological Sciences, University of the Sciences, Philadelphia, PA 19104, USA., Wu XS; Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA., Hammer JA 3rd; Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA., Liu Z; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA., English BP; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA., Mimori-Kiyosue Y; Optical Image Analysis Unit, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan., Romero DP; Department of Pharmacology, University of Minnesota, Minneapolis, MN 55455, USA., Ritter AT; Cell Biology and Metabolism Program, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA. Cambridge Institute for Medical Research, Addenbrooke's Hospital, Cambridge CB2 0XY, England, UK., Lippincott-Schwartz J; Cell Biology and Metabolism Program, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA., Fritz-Laylin L; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA., Mullins RD; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA., Mitchell DM; Department of Biochemistry and Cellular and Molecular Biology, University of Tennessee, Knoxville, TN 37996, USA., Bembenek JN; Department of Biochemistry and Cellular and Molecular Biology, University of Tennessee, Knoxville, TN 37996, USA., Reymann AC; Max Planck Institute for Molecular Cell Biology and Genetics, 01307 Dresden, Germany. Max Planck Institute for the Physics of Complex Systems, 01307 Dresden, Germany., Böhme R; Max Planck Institute for Molecular Cell Biology and Genetics, 01307 Dresden, Germany. Max Planck Institute for the Physics of Complex Systems, 01307 Dresden, Germany., Grill SW; Max Planck Institute for Molecular Cell Biology and Genetics, 01307 Dresden, Germany. Max Planck Institute for the Physics of Complex Systems, 01307 Dresden, Germany., Wang JT; Department of Molecular Biology and Genetics, Howard Hughes Medical Institute, Center for Cell Dynamics, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA., Seydoux G; Department of Molecular Biology and Genetics, Howard Hughes Medical Institute, Center for Cell Dynamics, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA., Tulu US; Department of Biology, Duke University, Durham, NC 27708, USA., Kiehart DP; Department of Biology, Duke University, Durham, NC 27708, USA., Betzig E; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA. betzige@janelia.hhmi.org.
المصدر: Science (New York, N.Y.) [Science] 2014 Oct 24; Vol. 346 (6208), pp. 1257998. Date of Electronic Publication: 2014 Oct 23.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 0404511 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9203 (Electronic) Linking ISSN: 00368075 NLM ISO Abbreviation: Science Subsets: MEDLINE
أسماء مطبوعة: Publication: : Washington, DC : American Association for the Advancement of Science
Original Publication: New York, N.Y. : [s.n.] 1880-
مواضيع طبية MeSH: Caenorhabditis elegans/*embryology , Drosophila melanogaster/*embryology , Embryo, Nonmammalian/*ultrastructure , Imaging, Three-Dimensional/*methods , Microscopy/*methods , Molecular Imaging/*methods, Animals ; Cell Communication ; Embryonic Stem Cells/ultrastructure ; Mice ; Spheroids, Cellular/ultrastructure
مستخلص: Although fluorescence microscopy provides a crucial window into the physiology of living specimens, many biological processes are too fragile, are too small, or occur too rapidly to see clearly with existing tools. We crafted ultrathin light sheets from two-dimensional optical lattices that allowed us to image three-dimensional (3D) dynamics for hundreds of volumes, often at subsecond intervals, at the diffraction limit and beyond. We applied this to systems spanning four orders of magnitude in space and time, including the diffusion of single transcription factor molecules in stem cell spheroids, the dynamic instability of mitotic microtubules, the immunological synapse, neutrophil motility in a 3D matrix, and embryogenesis in Caenorhabditis elegans and Drosophila melanogaster. The results provide a visceral reminder of the beauty and the complexity of living systems.
(Copyright © 2014, American Association for the Advancement of Science.)
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معلومات مُعتمدة: R01 HD037047 United States HD NICHD NIH HHS; RM01-GM61010 United States GM NIGMS NIH HHS; R01GM080370 United States GM NIGMS NIH HHS; GM33830 United States GM NIGMS NIH HHS; R01 GM033830 United States GM NIGMS NIH HHS; United States ImNIH Intramural NIH HHS; United States HHMI Howard Hughes Medical Institute; T32 GM007445 United States GM NIGMS NIH HHS; R01 GM080370 United States GM NIGMS NIH HHS; R01HD37047 United States HD NICHD NIH HHS; 281903 International ERC_ European Research Council
تواريخ الأحداث: Date Created: 20141025 Date Completed: 20141111 Latest Revision: 20220129
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC4336192
DOI: 10.1126/science.1257998
PMID: 25342811
قاعدة البيانات: MEDLINE
الوصف
تدمد:1095-9203
DOI:10.1126/science.1257998