دورية أكاديمية
أعرض في EDS

Multiple species of Trichosporon produce biofilms highly resistant to triazoles and amphotericin B.

التفاصيل البيبلوغرافية
العنوان: Multiple species of Trichosporon produce biofilms highly resistant to triazoles and amphotericin B.
المؤلفون: Iturrieta-González IA; Laboratório Especial de Micologia, Disciplina de Infectologia, Universidade Federal de São Paulo, São Paulo, SP, Brazil., Padovan AC; Laboratório Especial de Micologia, Disciplina de Infectologia, Universidade Federal de São Paulo, São Paulo, SP, Brazil; Departamento de Microbiologia e Imunologia, Instituto de Ciências Biomédicas, Universidade Federal de Alfenas, Alfenas, MG, Brazil., Bizerra FC; Laboratório Especial de Micologia, Disciplina de Infectologia, Universidade Federal de São Paulo, São Paulo, SP, Brazil., Hahn RC; Laboratório de Micologia, Faculdade de Medicina, Universidade Federal do Mato Grosso, Cuiabá, MT, Brazil., Colombo AL; Laboratório Especial de Micologia, Disciplina de Infectologia, Universidade Federal de São Paulo, São Paulo, SP, Brazil.
المصدر: PloS one [PLoS One] 2014 Oct 31; Vol. 9 (10), pp. e109553. Date of Electronic Publication: 2014 Oct 31 (Print Publication: 2014).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Amphotericin B/*pharmacology , Antifungal Agents/*pharmacology , Biofilms/*drug effects , Trichosporon/*drug effects, Blood/microbiology ; Brazil ; DNA, Ribosomal ; Drug Resistance, Fungal/drug effects ; Humans ; Microbial Sensitivity Tests ; Microscopy, Electron, Scanning ; Triazoles/pharmacology ; Trichosporon/isolation & purification ; Trichosporon/physiology ; Trichosporonosis/microbiology ; Urine/microbiology
مستخلص: Invasive infections caused by Trichosporon spp. have increased considerably in recent years, especially in neutropenic and critically ill patients using catheters and antibiotics. The genus presents limited sensitivity to different antifungal agents, but triazoles are the first choice for treatment. Here, we investigated the biofilm production and antifungal susceptibility to triazoles and amphotericin B of 54 Trichosporon spp. isolates obtained from blood samples (19), urine (20) and superficial mycosis (15). All isolates and 7 reference strains were identified by sequence analysis and phylogenetic inferences of the IGS1 region of the rDNA. Biofilms were grown on 96-well plates and quantitation was performed using crystal violet staining, complemented with Scanning Electron Microscopy (SEM). Susceptibility tests for fluconazole, itraconazole, voriconazole and amphotericin B were processed using the microdilution broth method (CLSI) for planktonic cells and XTT reduction assay for biofilm-forming cells. Our results showed that T. asahii was the most frequent species identified (66.7%), followed by T. faecale (11.1%), T. asteroides (9.3%), T. inkin (7.4%), T. dermatis (3.7%) and one T. coremiiforme (1.8%). We identified 4 genotypes within T. asahii isolates (G1, G3, G4 and G5) and 2 genotypes within T. faecale (G1 and G3). All species exhibited high adhesion and biofilm formation capabilities, mainly T. inkin, T. asteroides and T. faecale. Microscopy images of high biofilm-producing isolates showed that T. asahii presented mainly hyphae and arthroconidia, whereas T. asteroides exhibited mainly short arthroconidia and few filaments. Voriconazole exhibited the best in vitro activity against all species tested. Biofilm-forming cells of isolates and reference strains were highly resistant to all antifungals tested. We concluded that levels of biofilm formation by Trichosporon spp. were similar or even greater than those described for the Candida genus. Biofilm-forming cells were at least 1,000 times more resistant to antifungals than planktonic cells, especially to voriconazole.
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المشرفين على المادة: 0 (Antifungal Agents)
0 (DNA, Ribosomal)
0 (Triazoles)
7XU7A7DROE (Amphotericin B)
تواريخ الأحداث: Date Created: 20141101 Date Completed: 20150916 Latest Revision: 20181113
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC4215839
DOI: 10.1371/journal.pone.0109553
PMID: 25360765
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0109553