دورية أكاديمية
Efficacy of fish embryo vitrification protocols in terms of embryo morphology - a systematic review.
| العنوان: | Efficacy of fish embryo vitrification protocols in terms of embryo morphology - a systematic review. |
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| المؤلفون: | Souza de Carvalho AF; Department of Veterinary Medicine, Federal University of Lavras, Lavras, Minas Gerais, Brazil. alineferreirascarvalho@gmail.com., Ramos SE; Department of Veterinary Medicine, Federal University of Lavras, Lavras, Minas Gerais, Brazil., Gonzaga de Carvalho TS; Department of Veterinary Medicine, Federal University of Lavras, Lavras, Minas Gerais, Brazil., Pomarico de Souza YC; Departament of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, Minas Gerais, Brazil., Zangeronimo MG; Department of Veterinary Medicine, Federal University of Lavras, Lavras, Minas Gerais, Brazil., Pereira LJ; Department of Veterinary Medicine, Federal University of Lavras, Lavras, Minas Gerais, Brazil., Solis Murgas LD; Department of Veterinary Medicine, Federal University of Lavras, Lavras, Minas Gerais, Brazil. |
| المصدر: | Cryo letters [Cryo Letters] 2014 Sep-Oct; Vol. 35 (5), pp. 361-70. |
| نوع المنشور: | Journal Article; Systematic Review |
| اللغة: | English |
| بيانات الدورية: | Publisher: CryoLetters Country of Publication: England NLM ID: 9891832 Publication Model: Print Cited Medium: Print ISSN: 0143-2044 (Print) Linking ISSN: 01432044 NLM ISO Abbreviation: Cryo Letters Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London, Eng. : CryoLetters Original Publication: [London : Royal Veterinary College, |
| مواضيع طبية MeSH: | Vitrification*, Cryopreservation/*methods , Cryopreservation/*veterinary , Cryoprotective Agents/*metabolism , Embryo, Nonmammalian/*physiology , Fishes/*embryology, Animals ; Cryopreservation/instrumentation ; Cryoprotective Agents/toxicity ; Embryo, Nonmammalian/drug effects ; Embryo, Nonmammalian/embryology |
| مستخلص: | Background: Embryo cryopreservation has been used for the creation of genetic banks with diploid resources, and among different techniques, vitrification is considered as the most promising method. Objective: The goal is to evaluate the major aspects of the existing vitrification techniques and to evaluate their efficacy in terms of embryo morphology. Methods: Electronic searches in the PubMed and ScienceDirect databases were performed with the keyword combination: fish, embryo and vitrification. Pubmed retrieved 26 articles and Science Direct resulted in 464 articles. For this review, only studies that developed and tested vitrification protocols in fish embryos were included. Research regarding cryoprotectant toxicity and permeability were excluded. There were no restrictions on publication date or language. With these criteria, a total of ten articles were evaluated. Results: In these articles, the major aspects to be considered for the development of new vitrification protocols are: the cryoprotectants' toxicity, the embryos' development stage, the exposure to and the permeability of the cryoprotectants, vitrification devices and vitrification-warning cycle. Conclusion: The survival were limited, however, the preservation of embryonic morphology after thawing indicates the possibility of preserving fish embryos via the vitrification technique. |
| المشرفين على المادة: | 0 (Cryoprotective Agents) |
| تواريخ الأحداث: | Date Created: 20141115 Date Completed: 20150220 Latest Revision: 20240229 |
| رمز التحديث: | 20240229 |
| PMID: | 25397950 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0143-2044 |
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