دورية أكاديمية
Assessing impacts of unconventional natural gas extraction on microbial communities in headwater stream ecosystems in Northwestern Pennsylvania.
العنوان: | Assessing impacts of unconventional natural gas extraction on microbial communities in headwater stream ecosystems in Northwestern Pennsylvania. |
---|---|
المؤلفون: | Trexler R; Juniata College, Department of Biology Huntingdon, PA, USA., Solomon C; Juniata College, Department of Biology Huntingdon, PA, USA., Brislawn CJ; Juniata College, Department of Biology Huntingdon, PA, USA., Wright JR; Juniata College, Department of Biology Huntingdon, PA, USA., Rosenberger A; Juniata College, Department of Biology Huntingdon, PA, USA., McClure EE; Juniata College, Department of Biology Huntingdon, PA, USA., Grube AM; Juniata College, Department of Biology Huntingdon, PA, USA., Peterson MP; Juniata College, Department of Biology Huntingdon, PA, USA ; Department of Biology and Huck Institutes of Life Sciences, Pennsylvania State University University Park, PA, USA., Keddache M; DNA Sequencing and Genotyping Facility, Cincinnati Children's Hospital Medical Center Cincinnati, OH, USA., Mason OU; Department of Earth, Ocean, and Atmospheric Science, Florida State University Tallahassee, FL, USA., Hazen TC; Department of Civil and Environmental Engineering, University of Tennessee Knoxville Knoxville, TN, USA ; Biosciences Division, Oak Ridge National Laboratory Oak Ridge, TN, USA., Grant CJ; Juniata College, Department of Biology Huntingdon, PA, USA., Lamendella R; Juniata College, Department of Biology Huntingdon, PA, USA. |
المصدر: | Frontiers in microbiology [Front Microbiol] 2014 Nov 04; Vol. 5, pp. 522. Date of Electronic Publication: 2014 Nov 04 (Print Publication: 2014). |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101548977 Publication Model: eCollection Cited Medium: Print ISSN: 1664-302X (Print) Linking ISSN: 1664302X NLM ISO Abbreviation: Front Microbiol Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: Lausanne : Frontiers Research Foundation |
مستخلص: | Hydraulic fracturing and horizontal drilling have increased dramatically in Pennsylvania Marcellus shale formations, however the potential for major environmental impacts are still incompletely understood. High-throughput sequencing of the 16S rRNA gene was performed to characterize the microbial community structure of water, sediment, bryophyte, and biofilm samples from 26 headwater stream sites in northwestern Pennsylvania with different histories of fracking activity within Marcellus shale formations. Further, we describe the relationship between microbial community structure and environmental parameters measured. Approximately 3.2 million 16S rRNA gene sequences were retrieved from a total of 58 samples. Microbial community analyses showed significant reductions in species richness as well as evenness in sites with Marcellus shale activity. Beta diversity analyses revealed distinct microbial community structure between sites with and without Marcellus shale activity. For example, operational taxonomic units (OTUs) within the Acetobacteracea, Methylocystaceae, Acidobacteriaceae, and Phenylobacterium were greater than three log-fold more abundant in MSA+ sites as compared to MSA- sites. Further, several of these OTUs were strongly negatively correlated with pH and positively correlated with the number of wellpads in a watershed. It should be noted that many of the OTUs enriched in MSA+ sites are putative acidophilic and/or methanotrophic populations. This study revealed apparent shifts in the autochthonous microbial communities and highlighted potential members that could be responding to changing stream conditions as a result of nascent industrial activity in these aquatic ecosystems. |
References: | Genome Biol. 2011 Jun 24;12(6):R60. (PMID: 21702898) Science. 2001 Apr 6;292(5514):86-90. (PMID: 11292868) Front Microbiol. 2013 Dec 06;4:367. (PMID: 24367357) Appl Environ Microbiol. 2006 Jul;72(7):5069-72. (PMID: 16820507) Ecol Appl. 2009 Jul;19(5):1147-60. (PMID: 19688923) Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):4962-7. (PMID: 23479604) Environ Sci Technol. 2014;48(15):8334-48. (PMID: 24606408) J Am Water Resour Assoc. 2007 Feb;43(1):41-59. (PMID: 22457565) Appl Environ Microbiol. 2011 Jul;77(14):4744-53. (PMID: 21602366) Nat Methods. 2013 Oct;10(10):996-8. (PMID: 23955772) Microb Ecol. 1997 Nov;34(3):224-31. (PMID: 9337417) Gigascience. 2013 Nov 26;2(1):16. (PMID: 24280061) Ecology. 2007 Sep;88(9):2162-73. (PMID: 17918395) Microb Ecol. 2005 May;49(4):536-46. (PMID: 16052374) Microb Ecol. 1983 Jul;9(2):155-69. (PMID: 24221651) Microb Ecol. 2002 Jan;43(1):55-66. (PMID: 11984629) FEMS Microbiol Ecol. 2003 Dec 1;46(3):257-68. (PMID: 19719557) Appl Environ Microbiol. 1998 Jul;64(7):2739-42. (PMID: 9647861) Appl Environ Microbiol. 2001 Apr;67(4):1874-84. (PMID: 11282645) Proc Natl Acad Sci U S A. 2006 Jan 17;103(3):626-31. (PMID: 16407148) FEMS Microbiol Ecol. 2013 Dec;86(3):567-80. (PMID: 23875618) Ecol Appl. 2012 Apr;22(3):843-55. (PMID: 22645815) Microb Ecol. 2006 Feb;51(2):232-41. (PMID: 16463131) Nat Methods. 2010 May;7(5):335-6. (PMID: 20383131) PLoS One. 2013 Apr 22;8(4):e61217. (PMID: 23630581) Proc Natl Acad Sci U S A. 2011 Oct 25;108(43):E871; author reply E872. (PMID: 22003124) Microb Ecol. 2009 Jan;57(1):25-35. (PMID: 18592300) Int J Syst Evol Microbiol. 2012 Apr;62(Pt 4):977-83. (PMID: 21669924) Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11250-5. (PMID: 23798404) Environ Manage. 2012 May;49(5):1061-75. (PMID: 22447181) J Air Waste Manag Assoc. 2014 Jan;64(1):19-37. (PMID: 24620400) Microb Ecol. 2013 Jan;65(1):1-11. (PMID: 22903164) ISME J. 2010 Oct;4(10):1340-51. (PMID: 20445636) PLoS One. 2014 May 02;9(5):e96552. (PMID: 24794099) Ambio. 2014 Mar;43(2):218-33. (PMID: 24046144) Front Microbiol. 2012 Feb 14;3:35. (PMID: 22347877) Front Microbiol. 2012 Aug 17;3:303. (PMID: 22912630) Environ Sci Technol. 2013 Apr 2;47(7):3521-7. (PMID: 23441728) Environ Sci Technol. 2013 Mar 19;47(6):2562-9. (PMID: 23425120) Proc Natl Acad Sci U S A. 2012 Jul 24;109 (30):11961-6. (PMID: 22778445) Bioinformatics. 2010 Oct 1;26(19):2460-1. (PMID: 20709691) J Environ Sci Health A Tox Hazard Subst Environ Eng. 2015;50(5):482-500. (PMID: 25734824) Science. 2010 Oct 8;330(6001):204-8. (PMID: 20736401) MBio. 2013 Oct 22;4(5):e00708-13. (PMID: 24149512) Microb Ecol. 1999 Oct;38(3):234-243. (PMID: 10541785) Proc Natl Acad Sci U S A. 2011 Mar 15;108 Suppl 1:4516-22. (PMID: 20534432) Environ Microbiol. 2013 Sep;15(9):2517-31. (PMID: 23647974) |
فهرسة مساهمة: | Keywords: 16S rRNA gene sequencing; acidophilic; beta diversity; fracking; marcellus shale; methanotrophs; next generation sequencing |
تواريخ الأحداث: | Date Created: 20141120 Date Completed: 20141121 Latest Revision: 20200930 |
رمز التحديث: | 20231215 |
مُعرف محوري في PubMed: | PMC4219493 |
DOI: | 10.3389/fmicb.2014.00522 |
PMID: | 25408683 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1664-302X |
---|---|
DOI: | 10.3389/fmicb.2014.00522 |