دورية أكاديمية
أعرض في EDS

Increase in peroxisome number and the gene expression of putative glyoxysomal enzymes in Chlamydomonas cells supplemented with acetate.

التفاصيل البيبلوغرافية
العنوان: Increase in peroxisome number and the gene expression of putative glyoxysomal enzymes in Chlamydomonas cells supplemented with acetate.
المؤلفون: Hayashi Y; Faculty of Science, Niigata University, 8050 Ikarashi, Ninochou, Niigata, 950-2181, Japan, yhayashi@env.sc.niigata-u.ac.jp., Sato N, Shinozaki A, Watanabe M
المصدر: Journal of plant research [J Plant Res] 2015 Jan; Vol. 128 (1), pp. 177-85. Date of Electronic Publication: 2014 Nov 21.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Springer-Verlag Tokyo Country of Publication: Japan NLM ID: 9887853 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-0860 (Electronic) Linking ISSN: 09189440 NLM ISO Abbreviation: J Plant Res Subsets: MEDLINE
أسماء مطبوعة: Publication: 2002- : Tokyo ; New York : Springer-Verlag Tokyo
Original Publication: Tokyo : Botanical Society of Japan, c1993-
مواضيع طبية MeSH: Acetates/*pharmacology , Chlamydomonas/*enzymology , Chlamydomonas/*genetics , Gene Expression Regulation, Plant/*drug effects , Glyoxysomes/*enzymology , Peroxisomes/*metabolism, Cell Proliferation/drug effects ; Cells, Cultured ; Chlamydomonas/cytology ; Chlamydomonas/ultrastructure ; Culture Media/pharmacology ; Genes, Plant ; Glyoxysomes/drug effects ; Glyoxysomes/genetics ; Microscopy, Fluorescence ; Peroxisomes/drug effects ; Peroxisomes/ultrastructure ; RNA, Messenger/genetics ; RNA, Messenger/metabolism
مستخلص: We cultured Chlamydomonas reinhardtii cells in a minimal culture medium supplemented with various concentrations of acetate, fatty acids, ethanol, fatty alcohols, or sucrose. The presence of acetate (0.5 or 1.0%, w/v) was advantageous for cell growth. To determine whether peroxisomes are involved in fatty acid and fatty alcohol metabolism, we investigated the dynamics of peroxisomes, including changes in their number and size, in the presence of acetate, ethanol, and sucrose. The total volume of peroxisomes increased when cells were grown with acetate, but did not change when cells were grown with ethanol or sucrose. We analyzed cell growth on minimal culture medium supplemented with various fatty acids (carbon chain length ranging from one to ten) to investigate which fatty acids are metabolized by C. reinhardtii. Among them, acetate caused the greatest increase in growth when added to minimal culture media. We analyzed the transcript levels of genes encoding putative glyoxysomal enzymes. The transcript levels of genes encoding malate synthase, malate dehydrogenase, isocitrate lyase, and citrate synthase increased when Chlamydomonas cells were grown on minimal culture medium supplemented with acetate. Our results suggest that Chlamydomonas peroxisomes are involved in acetate metabolism via the glyoxylate cycle.
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المشرفين على المادة: 0 (Acetates)
0 (Culture Media)
0 (RNA, Messenger)
تواريخ الأحداث: Date Created: 20141122 Date Completed: 20160118 Latest Revision: 20181113
رمز التحديث: 20240628
DOI: 10.1007/s10265-014-0681-8
PMID: 25413007
قاعدة البيانات: MEDLINE
الوصف
تدمد:1618-0860
DOI:10.1007/s10265-014-0681-8