دورية أكاديمية
O-Phenanthroline as modulator of the hypoxic and catabolic response in cartilage tissue-engineering models.
| العنوان: | O-Phenanthroline as modulator of the hypoxic and catabolic response in cartilage tissue-engineering models. |
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| المؤلفون: | Georgi N; Department of Developmental BioEngineering, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, The Netherlands., Landman EB; Department of Developmental BioEngineering, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, The Netherlands., Klein TJ; Cartilage Regeneration Laboratory, Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Australia., van Blitterswijk CA; Department of Tissue Regeneration, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, The Netherlands., Karperien M; Department of Developmental BioEngineering, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, The Netherlands. |
| المصدر: | Journal of tissue engineering and regenerative medicine [J Tissue Eng Regen Med] 2017 Mar; Vol. 11 (3), pp. 724-732. Date of Electronic Publication: 2014 Nov 21. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: John Wiley & Sons Country of Publication: England NLM ID: 101308490 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1932-7005 (Electronic) Linking ISSN: 19326254 NLM ISO Abbreviation: J Tissue Eng Regen Med Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Chichester, West Sussex, UK : John Wiley & Sons |
| مواضيع طبية MeSH: | Models, Biological*, Cartilage, Articular/*pathology , Phenanthrolines/*pharmacology , Tissue Engineering/*methods, Animals ; Cell Count ; Cell Hypoxia/drug effects ; Chondrogenesis/drug effects ; Chondrogenesis/genetics ; Cobalt/pharmacology ; Extracellular Matrix/drug effects ; Extracellular Matrix/metabolism ; Extremities/embryology ; Gene Expression Regulation/drug effects ; Humans ; Hypoxia-Inducible Factor 1, alpha Subunit/metabolism ; Interleukin-1beta/pharmacology ; Male ; Mice ; Middle Aged ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; SOX9 Transcription Factor/genetics ; SOX9 Transcription Factor/metabolism ; Tumor Necrosis Factor-alpha/pharmacology |
| مستخلص: | Hypoxia has been shown to be important for maintaining cartilage homeostasis as well as for inducing chondrogenic differentiation. Ensuring low oxygen levels during in vitro culture is difficult, therefore we assessed the chondro-inductive capabilities of the hypoxia-mimicking agent O-phenanthroline, which is also known as a non-specific matrix metalloproteinase (MMP) inhibitor. We found that O-phenanthroline reduced the expression of MMP3 and MMP13 mRNA levels during chondrogenic differentiation of human chondrocytes (hChs), as well as after TNFα/IL-1β exposure in an explant model. Interestingly, O-phenanthroline significantly inhibited matrix degradation in a TNFα/IL-1β-dependent model of cartilage degeneration when compared to control and natural hypoxia (2.5% O (Copyright © 2014 John Wiley & Sons, Ltd.) |
| فهرسة مساهمة: | Keywords: cartilage; chondrogenesis; degradation; differentiation; phenanthroline |
| المشرفين على المادة: | 0 (HIF1A protein, human) 0 (Hypoxia-Inducible Factor 1, alpha Subunit) 0 (Interleukin-1beta) 0 (Phenanthrolines) 0 (RNA, Messenger) 0 (SOX9 Transcription Factor) 0 (Tumor Necrosis Factor-alpha) 3G0H8C9362 (Cobalt) EVS87XF13W (cobaltous chloride) W4X6ZO7939 (1,10-phenanthroline) |
| تواريخ الأحداث: | Date Created: 20141122 Date Completed: 20171120 Latest Revision: 20180129 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1002/term.1969 |
| PMID: | 25414128 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1932-7005 |
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| DOI: | 10.1002/term.1969 |