دورية أكاديمية
Novel mutations in the displacement loop of mitochondrial DNA are associated with acute lymphoblastic leukemia: a genetic sequencing study.
| العنوان: | Novel mutations in the displacement loop of mitochondrial DNA are associated with acute lymphoblastic leukemia: a genetic sequencing study. |
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| المؤلفون: | Yacoub HA; Department Cell Biology, Genetic Engineering and Biotechnology Division, National Research Center, Cairo, Egypt E-mail : haithamyacoub46@gmail.com., Mahmoud WM, El-Baz HA, Eid OM, ELfayoumi RI, Elhamidy SM, Mahmoud MM |
| المصدر: | Asian Pacific journal of cancer prevention : APJCP [Asian Pac J Cancer Prev] 2014; Vol. 15 (21), pp. 9283-9. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Asian Pacific Organization for Cancer Prevention Country of Publication: Thailand NLM ID: 101130625 Publication Model: Print Cited Medium: Internet ISSN: 2476-762X (Electronic) Linking ISSN: 15137368 NLM ISO Abbreviation: Asian Pac J Cancer Prev Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Bangkok : Asian Pacific Organization for Cancer Prevention, |
| مواضيع طبية MeSH: | Biomarkers, Tumor/*genetics , DNA, Mitochondrial/*genetics , Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics, Adolescent ; Adult ; Child ; Child, Preschool ; DNA Mutational Analysis ; Female ; Gene Frequency ; Humans ; Male ; Mutagenesis, Insertional ; Polymorphism, Single Nucleotide ; Saudi Arabia ; Young Adult |
| مستخلص: | Background: Acute lymphoblastic leukemia (ALL) is the most common cancer diagnosed in children and represents approximately 25% of cancer diagnoses among those younger than 15 years of age. Materials and Methods: This study investigated alterations in the displacement loop (d-loop) region of mitochondrial DNA (mtDNA) as a risk factor and diagnostic biomarker for early detection and diagnosis of acute lymphoblastic leukemia. Using mtDNA from 23 subjects diagnosed with acute lymphoblastic leukemia, the first 450 bp of the d-loop region were amplified and successfully sequenced. Results: This revealed 132 mutations at 25 positions in this region, with a mean of 6 alterations per subject. The d-loop alterations in mtDNA in subjects were all identified as single nucleotide polymorphisms in a homoplasmic distribution pattern. Mutant alleles were observed in all subjects with individual frequency rates of up to 95%. Thirteen mutant alleles in the d-loop region of mtDNA occurred with a high frequency. Novel alleles and locations were also identified in the d-loop of mtDNA as follows: 89 G insertions (40%), 95 G insertions (13%), 182 C/T substitutions (5%), 308 C insertions (19%), and 311 C insertions (80%). The findings of this study need to be replicated to be confirmed. Conclusions: Further investigation of the relationship between mutations in mitochondrial d-loop genes and incidence of acute lymphoblastic leukemia is recommended. |
| المشرفين على المادة: | 0 (Biomarkers, Tumor) 0 (DNA, Mitochondrial) |
| تواريخ الأحداث: | Date Created: 20141126 Date Completed: 20150715 Latest Revision: 20220408 |
| رمز التحديث: | 20221213 |
| DOI: | 10.7314/apjcp.2014.15.21.9283 |
| PMID: | 25422213 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2476-762X |
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| DOI: | 10.7314/apjcp.2014.15.21.9283 |