دورية أكاديمية
أعرض في EDS

Blocking ELISA using recombinant NcSRS2 protein for diagnosing bovine neosporosis.

التفاصيل البيبلوغرافية
العنوان: Blocking ELISA using recombinant NcSRS2 protein for diagnosing bovine neosporosis.
المؤلفون: Sinnott FA; Laboratório de Imunodiagnóstico, Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, CEP 96010-900, Pelotas, RS, P.O. Box 354, Brazil., Monte LG, Collares TF, De Matos BM, Pacheco DB, Borsuk S, Andreotti R, Hartleben CP
المصدر: Current microbiology [Curr Microbiol] 2015 Mar; Vol. 70 (3), pp. 429-32. Date of Electronic Publication: 2014 Nov 29.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Springer International Country of Publication: United States NLM ID: 7808448 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-0991 (Electronic) Linking ISSN: 03438651 NLM ISO Abbreviation: Curr Microbiol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: New York, Springer International.
مواضيع طبية MeSH: Enzyme-Linked Immunosorbent Assay*, Antigens, Protozoan/*immunology , Antigens, Surface/*immunology , Cattle Diseases/*diagnosis , Cattle Diseases/*immunology , Coccidiosis/*veterinary , Neospora/*immunology , Protozoan Proteins/*immunology, Animals ; Antibodies ; Antibodies, Blocking ; Cattle ; Cattle Diseases/parasitology ; Reproducibility of Results ; Sensitivity and Specificity
مستخلص: Neospora caninum is the etiologic agent of neosporosis, which leads to economic impacts on cattle industry. The reference method for serodiagnosis of neosporosis is the indirect fluorescent antibody test (IFAT). However, IFAT is laborious, expensive, and is not practicable in high throughput screening. In order to facilitate the serological diagnosis of neosporosis, we developed a blocking enzyme-linked immunosorbent assay (b-ELISA) based on NcSRS2 recombinant protein (rNcSRS2) and polyclonal antibodies against rNcSRS2 (b-ELISA/rNcSRS2). Compared to IFAT, b-ELISA/rNcSRS2 showed 93.7 % accuracy (98.7 % sensitivity and 88.7 % specificity), suggesting its potential as diagnostic assay to detect N. caninum antibodies in cattle sera.
References: J Clin Microbiol. 1996 Jun;34(6):1423-8. (PMID: 8735092)
Vet Parasitol. 2011 Aug 4;180(1-2):90-108. (PMID: 21704458)
Vet Parasitol. 2006 Aug 31;140(1-2):1-34. (PMID: 16730126)
J Vet Diagn Invest. 1995 Apr;7(2):273-5. (PMID: 7619917)
Vet Parasitol. 2010 Aug 4;171(3-4):337-42. (PMID: 20434268)
Korean J Parasitol. 2003 Mar;41(1):1-16. (PMID: 12666725)
Vet Parasitol. 2011 Apr 19;177(1-2):33-8. (PMID: 21168278)
Int J Parasitol. 2013 Feb;43(2):133-42. (PMID: 23246675)
J Am Vet Med Assoc. 1988 Nov 15;193(10):1259-63. (PMID: 3144521)
Clin Diagn Lab Immunol. 1998 Jul;5(4):467-73. (PMID: 9665950)
Vet Parasitol. 2013 Nov 8;197(3-4):477-86. (PMID: 23927916)
Vet Parasitol. 2013 Oct 18;197(1-2):346-9. (PMID: 23770068)
Vet Parasitol. 2012 Jun 8;187(1-2):28-35. (PMID: 22284302)
J Vet Med Sci. 2013 Nov;75(11):1413-8. (PMID: 23782543)
J Vet Diagn Invest. 2014 Jan;26(1):136-40. (PMID: 24398907)
Vet Parasitol. 2013 Sep 23;196(3-4):519-22. (PMID: 23566408)
Clin Vaccine Immunol. 2013 Oct;20(10 ):1617-22. (PMID: 23966554)
Vet Rec. 1993 Feb 6;132(6):125-6. (PMID: 8447050)
Rev Bras Parasitol Vet. 2009 Apr-Jun;18(2):19-22. (PMID: 19602311)
Curr Microbiol. 2014 Apr;68(4):472-6. (PMID: 24310934)
Pathog Glob Health. 2013 Apr;107(3):116-21. (PMID: 23683365)
BMC Biotechnol. 2012 May 04;12:19. (PMID: 22558916)
Vet Parasitol. 2012 Oct 26;189(2-4):211-7. (PMID: 22571832)
PLoS One. 2013;8(1):e53264. (PMID: 23308179)
المشرفين على المادة: 0 (Antibodies)
0 (Antibodies, Blocking)
0 (Antigens, Protozoan)
0 (Antigens, Surface)
0 (Protozoan Proteins)
0 (SRS2 protein, Neospora caninum)
تواريخ الأحداث: Date Created: 20141130 Date Completed: 20150922 Latest Revision: 20181113
رمز التحديث: 20231215
DOI: 10.1007/s00284-014-0737-y
PMID: 25432863
قاعدة البيانات: MEDLINE
الوصف
تدمد:1432-0991
DOI:10.1007/s00284-014-0737-y