دورية أكاديمية
أعرض في EDS

Erythroid progenitors from patients with low-risk myelodysplastic syndromes are dependent on the surrounding micro environment for their survival.

التفاصيل البيبلوغرافية
العنوان: Erythroid progenitors from patients with low-risk myelodysplastic syndromes are dependent on the surrounding micro environment for their survival.
المؤلفون: Folkerts H; Department of Hematology, University Medical Center Groningen, Groningen, The Netherlands., Hazenberg CL; Department of Hematology, University Medical Center Groningen, Groningen, The Netherlands., Houwerzijl EJ; Department of Internal Medicine, University Medical Center Groningen, Groningen, The Netherlands., van den Heuvel FA; Department of Hematology, University Medical Center Groningen, Groningen, The Netherlands; Department of Laboratory Medicine, University Medical Center Groningen, Groningen, The Netherlands., Mulder AB; Department of Laboratory Medicine, University Medical Center Groningen, Groningen, The Netherlands., van der Want JJ; Department of Cell Biology, University Medical Center Groningen, Groningen, The Netherlands; Department of Laboratory Medicine, Children's and Women's Health, Norwegian University of Science and Technology, Trondheim, Norway., Vellenga E; Department of Hematology, University Medical Center Groningen, Groningen, The Netherlands. Electronic address: e.vellenga@umcg.nl.
المصدر: Experimental hematology [Exp Hematol] 2015 Mar; Vol. 43 (3), pp. 215-222.e2. Date of Electronic Publication: 2014 Nov 20.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Science Inc Country of Publication: Netherlands NLM ID: 0402313 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2399 (Electronic) Linking ISSN: 0301472X NLM ISO Abbreviation: Exp Hematol Subsets: MEDLINE
أسماء مطبوعة: Publication: 1999- : Amsterdam : Elsevier Science Inc.
Original Publication: Copenhagen, Munksgaard.
مواضيع طبية MeSH: Tumor Microenvironment*, Erythroid Precursor Cells/*physiology , Myelodysplastic Syndromes/*physiopathology, Aged ; Aged, 80 and over ; Apoptosis ; Cell Survival ; Cells, Cultured ; Erythroid Precursor Cells/pathology ; Female ; Flow Cytometry ; Humans ; Leukocytes, Mononuclear/pathology ; Male ; Middle Aged ; Real-Time Polymerase Chain Reaction ; Risk Factors
مستخلص: To investigate whether the type of programmed cell death of myelodysplastic erythroid cells depends on their cellular context, we performed studies on cells from patients with low-risk myelodysplastic syndromes. We compared erythroid cells (and their precursor cells) from the mononuclear cell fraction with those from the hematon fraction, which are compacted complexes of hematopoietic cells surrounded by their own micro-environment. In directly fixed materials, erythroblasts exhibited signs of autophagy with limited apoptosis (<3%) based on ultrastructural characteristics and immunogold labeling for activated caspase-3. After 24 h in culture, myelodysplastic erythroblasts exhibited a significant increase in apoptosis (22 ± 7% vs. 3 ± 2%, p = 0.001). In contrast, the myelodysplastic erythroblasts from the hematon fraction did not exhibit an increased tendency toward apoptosis after culture (7 ± 3.3% vs. 1.8 ± 2.3%), which was in line with results for normal bone marrow cells. The same dependency on the micro-environment was noted for immature erythroid progenitor cells. Myelodysplastic hematons exhibited distinct numbers of erythroid burst-forming units in association with an extensive network of stromal cells, whereas small numbers of erythroid burst-forming units were generated from the myelodysplastic mononuclear cells compared with normal mononuclear cells (10.2 ± 9 vs. 162 ± 125, p < 0.001). Co-culture of erythroid myelodysplastic cells in the presence of growth factors (vascular endothelial growth factor, leukemia inhibitory factor) or on the MS-5 stromal layer did not restore the expansion of erythroid precursor cells. These data indicate that surviving myelodysplastic erythroid progenitors become more vulnerable to programmed cell death when they are detached from their own micro-environment.
(Copyright © 2015 ISEH - International Society for Experimental Hematology. Published by Elsevier Inc. All rights reserved.)
تواريخ الأحداث: Date Created: 20141203 Date Completed: 20150526 Latest Revision: 20150321
رمز التحديث: 20221213
DOI: 10.1016/j.exphem.2014.11.005
PMID: 25462021
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-2399
DOI:10.1016/j.exphem.2014.11.005