دورية أكاديمية
The visualization of large organized chromatin domains enriched in the H3K9me2 mark within a single chromosome in a single cell.
| العنوان: | The visualization of large organized chromatin domains enriched in the H3K9me2 mark within a single chromosome in a single cell. |
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| المؤلفون: | Chen X; a Department of Microbiology; Tumor and Cell Biology ; Stockholm , Sweden., Yammine S, Shi C, Tark-Dame M, Göndör A, Ohlsson R |
| المصدر: | Epigenetics [Epigenetics] 2014 Nov; Vol. 9 (11), pp. 1439-45. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Taylor & Francis Country of Publication: United States NLM ID: 101265293 Publication Model: Print Cited Medium: Internet ISSN: 1559-2308 (Electronic) Linking ISSN: 15592294 NLM ISO Abbreviation: Epigenetics Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : Philadelphia, PA : Taylor & Francis Original Publication: Georgetown, Tex. : Landes Bioscience, c2006- |
| مواضيع طبية MeSH: | Genetic Techniques*, Chromatin/*metabolism , Chromosomes, Human, Pair 11/*metabolism , Lysine/*metabolism, DNA Probes ; HCT116 Cells ; Histones/metabolism ; Humans ; Image Processing, Computer-Assisted ; Methylation ; Microscopy, Confocal ; Single-Cell Analysis/methods |
| مستخلص: | Despite considerable efforts, our understanding of the organization of higher order chromatin conformations in single cells and how these relate to chromatin marks remains poor. We have earlier invented the Chromatin In Situ Proximity (ChrISP) technique to determine proximities between chromatin fibers within a single chromosome. Here we used ChrISP to identify chromosome 11-specific hubs that are enriched in the H3K9me2 mark and that project toward the nuclear membrane in finger-like structures. Conversely, chromosome 11-specfic chromatin hubs, visualized by the presence of either H3K9me1 or H3K9me3 marks, are chromosome-wide and largely absent at the nuclear periphery. As the nuclear periphery-specific chromatin hubs were lost in the induced reduction of H3K9me2 levels, they likely represent Large Organization Chromatin in Lysine Methylation (LOCK) domains, previously identified by ChIP-seq analysis. Strikingly, the downregulation of the H3K9me2/3 marks also led to the chromosome-wide compaction of chromosome 11, suggesting a pleiotropic function of these features not recognized before. The ChrISP-mediated visualization of dynamic chromatin states in single cells thus provides an analysis of chromatin structures with a resolution far exceeding that of any other light microscopic technique. |
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| فهرسة مساهمة: | Keywords: 3C, chromatin conformation capture; CT11, Chromosome Territory 11; ChIP-seq, chromatin immunoprecipitation sequencing; ChrISP, chromatin in situ proximity; FISH, fluorescence in situ hybridization; LAD, lamin-associated domain; LOCK, Large Organization Chromatin in Lysine Methylation; chromatin; conformation; domains; epigenetics |
| المشرفين على المادة: | 0 (Chromatin) 0 (DNA Probes) 0 (Histones) K3Z4F929H6 (Lysine) |
| تواريخ الأحداث: | Date Created: 20141209 Date Completed: 20150803 Latest Revision: 20181113 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC4623470 |
| DOI: | 10.4161/15592294.2014.971633 |
| PMID: | 25482057 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1559-2308 |
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| DOI: | 10.4161/15592294.2014.971633 |