دورية أكاديمية
Nitric oxide-induced polycystic ovaries in the wistar rat.
| العنوان: | Nitric oxide-induced polycystic ovaries in the wistar rat. |
|---|---|
| المؤلفون: | Hassani F; Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran., Karami M; Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran., 1 PD; Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran., Jalali Nadoushan MR; Department of Pathology, School of Medicine, Shahed University, Tehran, Iran., Yazdi PE; Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. |
| المصدر: | International journal of fertility & sterility [Int J Fertil Steril] 2012 Jul; Vol. 6 (2), pp. 111-6. Date of Electronic Publication: 2012 Jun 19. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Royan Institute of Iran Country of Publication: Iran NLM ID: 101487941 Publication Model: Print-Electronic Cited Medium: Print ISSN: 2008-076X (Print) Linking ISSN: 20080778 NLM ISO Abbreviation: Int J Fertil Steril Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Tehran : Royan Institute of Iran |
| مستخلص: | Background: Nitric oxide (NO) involves in polycystic ovary syndrome (PCOS), a cause of infertility in women during the reproductive age. The PCOS is now categorized as an inflammatory phenomenon. The aim of this study was to evaluate the role of NO, a proinflammatory agent, in this syndrome at histological and biochemical levels. Materials and Methods: In this experimental study, animals were female Wistar rats (weighing 200-250 g) kept under standard conditions. L-Arginine (50-200 mg/kg), a precursor of NO, was injected intra-peritoneally (i.p.) through a period ranging from 9 to14 days/ once a day. The rats' estrous cycle was studied using Papanicolaou test; those showing phase of Diestrous were grouped into experimental and control groups. The control group solely received saline (1 ml/kg, i.p.) throughout all experiments. To evaluate the inflammatory effect of NO, the rats were treated an anti-inflammatory agent, naloxone hydrochloride (0.4 mg/kg, i.p.), prior to L-arginine. At the end of the treatment period all animals' ovaries were assessed for histopathological and histochemical investigations. Also, activation of NO synthase (NOS) in the experiments was studied using NADPH-diaphorase technique. Results: The ovaries of rats treated with L-arginine showed polycystic characteristics in contrast to those collected from control or naloxone pretreated groups, based on image analysis. A difference in enzyme activation was also shown in the sections that belonged to the groups that received L-arginine when compared with the pre-naloxone and control groups. Conclusion: Based on these results, we believe that NO may play a major role in the pathophysiology of PCOS. |
| References: | Hum Reprod. 2006 Mar;21(3):651-6. (PMID: 16311293) Eur J Obstet Gynecol Reprod Biol. 2007 Aug;133(2):191-6. (PMID: 17049715) J Clin Invest. 1993 Dec;92(6):3053-6. (PMID: 7504698) Biochem Biophys Res Commun. 1993 Sep 15;195(2):1035-40. (PMID: 7690549) Am J Physiol. 1995 Aug;269(2 Pt 1):E290-8. (PMID: 7544532) Endocrinology. 1994 Nov;135(5):2287-90. (PMID: 7525265) Biol Reprod. 1996 Feb;54(2):436-45. (PMID: 8788197) Fertil Steril. 1996 Dec;66(6):1036-8. (PMID: 8941078) Hum Reprod. 1996 Dec;11(12):2667-73. (PMID: 9021370) J Clin Endocrinol Metab. 1998 May;83(5):1742-5. (PMID: 9589685) Fertil Steril. 2002 May;77(5):936-44. (PMID: 12009347) Biotech Histochem. 2005 Mar-Apr;80(2):79-87. (PMID: 16195173) Reprod Biol Endocrinol. 2003 Apr 08;1:33. (PMID: 12725645) Braz J Biol. 2002 Nov;62(4A):609-14. (PMID: 12659010) J Am Coll Cardiol. 2006 Nov 7;48(9):1871-9. (PMID: 17084265) Physiol Res. 2007;56(1):67-78. (PMID: 16497098) Arch Med Res. 2006 Oct;37(7):830-9. (PMID: 16971221) Curr Pharm Des. 2006;12(8):1001-12. (PMID: 16533167) Endocrinology. 2007 Aug;148(8):3781-91. (PMID: 17495003) Endocr J. 1999 Aug;46(4):529-38. (PMID: 10580745) Endocrinology. 1994 Nov;135(5):1799-806. (PMID: 7525252) Histochemistry. 1982;75(2):163-7. (PMID: 7129965) |
| فهرسة مساهمة: | Keywords: L-Arginine; NADPH-Diaphorase; Naloxone; Nitric oxide; PCOS |
| تواريخ الأحداث: | Date Created: 20141211 Date Completed: 20151026 Latest Revision: 20200929 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC4258239 |
| PMID: | 25493168 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2008-076X |
|---|