دورية أكاديمية
Nitric oxide-induced polycystic ovaries in the wistar rat.
العنوان: | Nitric oxide-induced polycystic ovaries in the wistar rat. |
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المؤلفون: | Hassani F; Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran., Karami M; Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran., 1 PD; Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran., Jalali Nadoushan MR; Department of Pathology, School of Medicine, Shahed University, Tehran, Iran., Yazdi PE; Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. |
المصدر: | International journal of fertility & sterility [Int J Fertil Steril] 2012 Jul; Vol. 6 (2), pp. 111-6. Date of Electronic Publication: 2012 Jun 19. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Royan Institute of Iran Country of Publication: Iran NLM ID: 101487941 Publication Model: Print-Electronic Cited Medium: Print ISSN: 2008-076X (Print) Linking ISSN: 20080778 NLM ISO Abbreviation: Int J Fertil Steril Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: Tehran : Royan Institute of Iran |
مستخلص: | Background: Nitric oxide (NO) involves in polycystic ovary syndrome (PCOS), a cause of infertility in women during the reproductive age. The PCOS is now categorized as an inflammatory phenomenon. The aim of this study was to evaluate the role of NO, a proinflammatory agent, in this syndrome at histological and biochemical levels. Materials and Methods: In this experimental study, animals were female Wistar rats (weighing 200-250 g) kept under standard conditions. L-Arginine (50-200 mg/kg), a precursor of NO, was injected intra-peritoneally (i.p.) through a period ranging from 9 to14 days/ once a day. The rats' estrous cycle was studied using Papanicolaou test; those showing phase of Diestrous were grouped into experimental and control groups. The control group solely received saline (1 ml/kg, i.p.) throughout all experiments. To evaluate the inflammatory effect of NO, the rats were treated an anti-inflammatory agent, naloxone hydrochloride (0.4 mg/kg, i.p.), prior to L-arginine. At the end of the treatment period all animals' ovaries were assessed for histopathological and histochemical investigations. Also, activation of NO synthase (NOS) in the experiments was studied using NADPH-diaphorase technique. Results: The ovaries of rats treated with L-arginine showed polycystic characteristics in contrast to those collected from control or naloxone pretreated groups, based on image analysis. A difference in enzyme activation was also shown in the sections that belonged to the groups that received L-arginine when compared with the pre-naloxone and control groups. Conclusion: Based on these results, we believe that NO may play a major role in the pathophysiology of PCOS. |
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فهرسة مساهمة: | Keywords: L-Arginine; NADPH-Diaphorase; Naloxone; Nitric oxide; PCOS |
تواريخ الأحداث: | Date Created: 20141211 Date Completed: 20151026 Latest Revision: 20200929 |
رمز التحديث: | 20231215 |
مُعرف محوري في PubMed: | PMC4258239 |
PMID: | 25493168 |
قاعدة البيانات: | MEDLINE |
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