دورية أكاديمية
أعرض في EDS

Genetic and functional diversity of propagating cells in glioblastoma.

التفاصيل البيبلوغرافية
العنوان: Genetic and functional diversity of propagating cells in glioblastoma.
المؤلفون: Piccirillo SGM; John van Geest Centre for Brain Repair, Department of Clinical Neurosciences, University of Cambridge, Cambridge CB2 0PY, UK., Colman S; Centre for Evolution and Cancer, Division of Molecular Pathology, The Institute of Cancer Research, London SM2 5NG, UK., Potter NE; Centre for Evolution and Cancer, Division of Molecular Pathology, The Institute of Cancer Research, London SM2 5NG, UK., van Delft FW; Centre for Evolution and Cancer, Division of Molecular Pathology, The Institute of Cancer Research, London SM2 5NG, UK., Lillis S; Molecular Diagnostics, The Centre for Molecular Pathology, The Royal Marsden NHS Foundation Trust, London SM2 5PT, UK., Carnicer MJ; Centre for Evolution and Cancer, Division of Molecular Pathology, The Institute of Cancer Research, London SM2 5NG, UK., Kearney L; Centre for Evolution and Cancer, Division of Molecular Pathology, The Institute of Cancer Research, London SM2 5NG, UK., Watts C; John van Geest Centre for Brain Repair, Department of Clinical Neurosciences, University of Cambridge, Cambridge CB2 0PY, UK; Division of Neurosurgery, Department of Clinical Neurosciences, University of Cambridge, Box 167 Addenbrooke's Hospital, Hills Road, Cambridge CB2 0QQ, UK. Electronic address: cw209@cam.ac.uk., Greaves M; Centre for Evolution and Cancer, Division of Molecular Pathology, The Institute of Cancer Research, London SM2 5NG, UK. Electronic address: mel.greaves@icr.ac.uk.
المصدر: Stem cell reports [Stem Cell Reports] 2015 Jan 13; Vol. 4 (1), pp. 7-15. Date of Electronic Publication: 2014 Dec 18.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Cell Press Country of Publication: United States NLM ID: 101611300 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2213-6711 (Electronic) Linking ISSN: 22136711 NLM ISO Abbreviation: Stem Cell Reports Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Cambridge, MA] : Cell Press, c 2013-
مواضيع طبية MeSH: Genetic Variation* , Phenotype*, Brain Neoplasms/*genetics , Brain Neoplasms/*metabolism , Glioblastoma/*genetics , Glioblastoma/*metabolism, Animals ; Brain Neoplasms/pathology ; Cell Line, Tumor ; DNA Copy Number Variations ; Disease Progression ; Genome-Wide Association Study ; Genomics ; Glioblastoma/pathology ; Heterografts ; High-Throughput Nucleotide Sequencing ; Humans ; In Situ Hybridization, Fluorescence ; Mice ; Neoplastic Stem Cells/metabolism ; Neoplastic Stem Cells/pathology ; Polymorphism, Single Nucleotide ; Single-Cell Analysis
مستخلص: Glioblastoma (GBM) is a lethal malignancy whose clinical intransigence has been linked to extensive intraclonal genetic and phenotypic diversity and the common emergence of therapeutic resistance. This interpretation embodies the implicit assumption that cancer stem cells or tumor-propagating cells are themselves genetically and functionally diverse. To test this, we screened primary GBM tumors by SNP array to identify copy number alterations (a minimum of three) that could be visualized in single cells by multicolor fluorescence in situ hybridization. Interrogation of neurosphere-derived cells (from four patients) and cells derived from secondary transplants of these same cells in NOD-SCID mice allowed us to infer the clonal and phylogenetic architectures. Whole-exome sequencing and single-cell genetic analysis in one case revealed a more complex clonal structure. This proof-of-principle experiment revealed that subclones in each GBM had variable regenerative or stem cell activity, and highlighted genetic alterations associated with more competitive propagating activity in vivo.
(Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.)
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معلومات مُعتمدة: 105104 United Kingdom WT_ Wellcome Trust; 16700 United Kingdom CRUK_ Cancer Research UK; G108/507 United Kingdom MRC_ Medical Research Council
تواريخ الأحداث: Date Created: 20141224 Date Completed: 20150723 Latest Revision: 20240210
رمز التحديث: 20240210
مُعرف محوري في PubMed: PMC4297869
DOI: 10.1016/j.stemcr.2014.11.003
PMID: 25533637
قاعدة البيانات: MEDLINE
الوصف
تدمد:2213-6711
DOI:10.1016/j.stemcr.2014.11.003