دورية أكاديمية
A cytofluorometric study of membrane rafts in human monocyte subsets in atherosclerosis.
| العنوان: | A cytofluorometric study of membrane rafts in human monocyte subsets in atherosclerosis. |
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| المؤلفون: | Chelombitko MA; Biological Faculty, Lomonosov Moscow State University, Leninskie Gory 1, Bldg. 12, Moscow, 119991, Russia ; Russian Cardiology Research and Production Complex, 3rd Cherepkovskaya Str., 15A, Moscow, 121552, Russia., Shishkina VS; Biological Faculty, Lomonosov Moscow State University, Leninskie Gory 1, Bldg. 12, Moscow, 119991, Russia ; Russian Cardiology Research and Production Complex, 3rd Cherepkovskaya Str., 15A, Moscow, 121552, Russia., Ilyinskaya OP; Biological Faculty, Lomonosov Moscow State University, Leninskie Gory 1, Bldg. 12, Moscow, 119991, Russia., Kaminnyi AI; Russian Cardiology Research and Production Complex, 3rd Cherepkovskaya Str., 15A, Moscow, 121552, Russia., Pavlunina TO; Russian Cardiology Research and Production Complex, 3rd Cherepkovskaya Str., 15A, Moscow, 121552, Russia., Samovilova NN; Russian Cardiology Research and Production Complex, 3rd Cherepkovskaya Str., 15A, Moscow, 121552, Russia., Gracheva EV; Russian Cardiology Research and Production Complex, 3rd Cherepkovskaya Str., 15A, Moscow, 121552, Russia., Tararak EM; Russian Cardiology Research and Production Complex, 3rd Cherepkovskaya Str., 15A, Moscow, 121552, Russia., Prokazova NV; Russian Cardiology Research and Production Complex, 3rd Cherepkovskaya Str., 15A, Moscow, 121552, Russia. |
| المصدر: | Acta naturae [Acta Naturae] 2014 Oct; Vol. 6 (4), pp. 80-8. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: National Research University Higher School of Economics Country of Publication: Russia (Federation) NLM ID: 101525823 Publication Model: Print Cited Medium: Print ISSN: 2075-8251 (Print) Linking ISSN: 20758251 NLM ISO Abbreviation: Acta Naturae Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Publication: 2019- : [Moscow] : National Research University Higher School of Economics Original Publication: Moscow : Park Media Ltd |
| مستخلص: | The peripheral blood monocytes of atherosclerotic patients are pre-activated and have some of the features of tissue macrophages. Their adhesion to the endothelium is 1.5 times higher than that of monocytes from healthy subjects, and they express a number of receptors and antigens typical of tissue macrophages. Additionally, earlier we showed that the biosynthesis of gangliosides, whose main function is the formation of membrane rafts, is significantly activated in blood monocytes from atherosclerotic patients, as well as during the in vitro differentiation of normal monocytes into macrophages. In this study, we investigated the expression of membrane rafts on various monocyte subsets from healthy subjects and atherosclerotic patients. Based on flow cytometry results, the monocytes in the examined atherosclerotic patients were found to differ from those in healthy subjects by a twofold increase in the proportion of the intermediate subset (CD14(++)/CD16(+)) and by enhancement in the expression of the fractalkine receptor CX3CR1 on the intermediate and non-classical subsets (CD14(++)/CD16(+) and CD14(+)/CD16(++)) (2.3 and 1.8 times, respectively). This suggests a pre-activated state of monocytes in atherosclerotic patients. At the same time, the expression of the membrane raft marker on the monocyte subsets was similar in both studied groups. However, a study of the in vitro differentiation of monocytes into macrophages showed that the membrane raft expression increased 2 times as early as on the 1st day of culturing and 3 times on the 7th day compared to that in freshly isolated monocytes. Therefore, it is suggested that monocytes in atherosclerosis accumulate gangliosides that are used to form membrane rafts during the macrophage differentiation after the migration of monocytes into the arterial intima. |
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| فهرسة مساهمة: | Keywords: atherosclerosis; flow cytometry; macrophages; membrane rafts; monocyte subsets |
| تواريخ الأحداث: | Date Created: 20150106 Date Completed: 20150105 Latest Revision: 20240510 |
| رمز التحديث: | 20240510 |
| مُعرف محوري في PubMed: | PMC4273095 |
| PMID: | 25558398 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2075-8251 |
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