دورية أكاديمية
Human breast adipose-derived stem cells transfected with the stromal cell-derived factor-1 receptor CXCR4 exhibit enhanced viability in human autologous free fat grafts.
| العنوان: | Human breast adipose-derived stem cells transfected with the stromal cell-derived factor-1 receptor CXCR4 exhibit enhanced viability in human autologous free fat grafts. |
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| المؤلفون: | Xu FT; Department of Orthopedics, the First Affiliated Hospital of Gannan Medical University, Ganzhou, China., Li HM, Yin QS, Liu DL, Nan H, Zhao PR, Liang SW |
| المصدر: | Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology [Cell Physiol Biochem] 2014; Vol. 34 (6), pp. 2091-104. Date of Electronic Publication: 2014 Nov 28. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Physiol Biochem Press GmbH & Co KG Country of Publication: Germany NLM ID: 9113221 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1421-9778 (Electronic) Linking ISSN: 10158987 NLM ISO Abbreviation: Cell Physiol Biochem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2019- : Düsseldorf, Germany : Cell Physiol Biochem Press GmbH & Co KG Original Publication: Basel ; New York : S. Karger, 1991-2018. |
| مواضيع طبية MeSH: | Stem Cell Transplantation* , Transplantation, Autologous*, Cell Proliferation/*genetics , Receptors, CXCR4/*metabolism , Stromal Cells/*cytology, Adipose Tissue/cytology ; Adipose Tissue/metabolism ; Animals ; Apoptosis/genetics ; Breast/cytology ; Cell Survival/genetics ; Chemokine CXCL12/genetics ; Graft Survival ; Humans ; Mice |
| مستخلص: | Background: The main complication of autologous free fat tissue transplantation is fat resorption and calcification due to the ischemic necrosis of fat. The promotion of transplant neovascularization soon after autologous free fat grafts may reduce these outcomes. In adulthood, stromal cell-derived factor-1 (SDF-1) and its membrane receptor C-X-C chemokine receptor type 4 (CXCR4) are involved in the homing and migration of multiple stem cell types, neovascularization, and cell proliferation. We hypothesized that CXCR4 may improve the long-term survival of free fat tissue transplants by recruiting endothelial progenitor cells (EPCs) and may therefore improve graft revascularization. In this study, we aimed to determine the effect of human breast adipose-derived stem cells (HBASCs) transfected with the CXCR4 gene on the survival rate of human autologous free fat transplants in nude mice. Methods: Human breast adipose-derived stem cells (HBASCs) were expanded ex vivo for 3 passages, labeled with green fluorescent protein (GFP) and transfected with CXCR4 or left untransfected. Autologous fat tissues were mixed with the GFP-labeled, CXCR4-transfected HBASCs (group A), GFP-labeled HBASCs (group B), the known vascularization-promoting agent VEGF (group C), or medium (group D) and then injected subcutaneously into 32 nude mice at 4 spots in a random fashion. Six months later, the transplanted tissue volume and histology were evaluated, and neo-vascularization was quantified by counting the capillaries. CXCR4 and SDF-1α mRNA expression in the transplants was determined using real-time quantitative PCR analysis (qPCR). Results: The data revealed that the control (group D) transplant volume survival was 28.3 ± 4.5%. Mixing CXCR4-transfected (group A) and untransfected (group B) HBASCs significantly increased transplant volume survival (79.5 ± 8.3% and 67.2 ± 5.9%, respectively), whereas VEGF-transfected HBASCs (group C) were less effective (41.2 ± 5.1%). Histological analysis revealed that both types of HBASCs-treated transplants consisted predominantly of adipose tissue, unlike the control transplants, and also presented significantly less fat necrosis and fibrosis. The CXCR4-transfected HBASCs-treated transplants had a significantly higher capillary density than did the other transplants and showed GFP and CD31 double-positive cells (i.e., ASCs-derived endothelial cells). The mRNA expression of CXCR4 and SDF-1α was much higher in the CXCR4-transfected HBASCs transplants than in the other three transplants. Conclusions: Our data demonstrated that HBASCs can enhance the survival and quality of transplanted free fat tissues. Moreover, CXCR4 transfection of these HBASCs could augment this effect. Stimulation of angiogenesis and decreased fat cell apoptosis due to the recruitment of endothelial progenitor cells (EPCs) and an increase in graft revascularization are potential mechanisms underlying the improved long-term survival of free fat transplants following CXCR4-transfected HBASCs treatment. |
| المشرفين على المادة: | 0 (CXCL12 protein, human) 0 (CXCR4 protein, human) 0 (Chemokine CXCL12) 0 (Receptors, CXCR4) |
| تواريخ الأحداث: | Date Created: 20150107 Date Completed: 20150928 Latest Revision: 20190212 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1159/000366404 |
| PMID: | 25562157 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1421-9778 |
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| DOI: | 10.1159/000366404 |