دورية أكاديمية
أعرض في EDS

Structural bisphenol analogues differentially target steroidogenesis in murine MA-10 Leydig cells as well as the glucocorticoid receptor.

التفاصيل البيبلوغرافية
العنوان: Structural bisphenol analogues differentially target steroidogenesis in murine MA-10 Leydig cells as well as the glucocorticoid receptor.
المؤلفون: Roelofs MJ; Endocrine Toxicology Research Group, Institute for Risk Assessment Sciences (IRAS), Utrecht University, P.O. Box 80.177, NL-3508 TD Utrecht, The Netherlands; Center for Health Protection, National Institute for Public Health and the Environment (RIVM), P.O. Box 1, 3720 BA Bilthoven, The Netherlands. Electronic address: m.j.e.roelofs@uu.nl., van den Berg M; Endocrine Toxicology Research Group, Institute for Risk Assessment Sciences (IRAS), Utrecht University, P.O. Box 80.177, NL-3508 TD Utrecht, The Netherlands., Bovee TF; RIKILT-Institute of Food Safety, Wageningen University and Research Centre, Akkermaalsbos 2, 6708 WB Wageningen, The Netherlands., Piersma AH; Center for Health Protection, National Institute for Public Health and the Environment (RIVM), P.O. Box 1, 3720 BA Bilthoven, The Netherlands; Endocrine Toxicology Research Group, Institute for Risk Assessment Sciences (IRAS), Utrecht University, P.O. Box 80.177, NL-3508 TD Utrecht, The Netherlands., van Duursen MB; Endocrine Toxicology Research Group, Institute for Risk Assessment Sciences (IRAS), Utrecht University, P.O. Box 80.177, NL-3508 TD Utrecht, The Netherlands.
المصدر: Toxicology [Toxicology] 2015 Mar 02; Vol. 329, pp. 10-20. Date of Electronic Publication: 2015 Jan 08.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Ireland NLM ID: 0361055 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3185 (Electronic) Linking ISSN: 0300483X NLM ISO Abbreviation: Toxicology Subsets: MEDLINE
أسماء مطبوعة: Publication: Limerick : Elsevier
Original Publication: Amsterdam. Elsevier/North-Holland.
مواضيع طبية MeSH: Benzhydryl Compounds/*toxicity , Leydig Cells/*drug effects , Phenols/*toxicity , Receptors, Glucocorticoid/*metabolism, Animals ; Biological Assay ; Cell Survival/drug effects ; Cells, Cultured ; Endocrine Disruptors/toxicity ; Gene Expression ; Leydig Cells/metabolism ; Male ; Mice ; Polybrominated Biphenyls/toxicity ; Receptors, Androgen/genetics ; Receptors, Androgen/metabolism ; Receptors, Glucocorticoid/genetics ; Sulfones/toxicity ; Testis/drug effects ; Testis/metabolism ; Testosterone/metabolism ; Yeasts/metabolism
مستخلص: Although much information on the endocrine activity of bisphenol A (BPA) is available, a proper human hazard assessment of analogues that are believed to have a less harmful toxicity profile is lacking. Here the possible effects of BPA, bisphenol F (BPF), bisphenol S (BPS), as well as the brominated structural analogue and widely used flame retardant tetrabromobisphenol A (TBBPA) on human glucocorticoid and androgen receptor (GR and AR) activation were assessed. BPA, BPF, and TBBPA showed clear GR and AR antagonism with IC50 values of 67 μM, 60 μM, and 22 nM for GR, and 39 μM, 20 μM, and 982 nM for AR, respectively, whereas BPS did not affect receptor activity. In addition, murine MA-10 Leydig cells exposed to the bisphenol analogues were assessed for changes in secreted steroid hormone levels. Testicular steroidogenesis was altered by all bisphenol analogues tested. TBBPA effects were more directed towards the male end products and induced testosterone synthesis, while BPF and BPS predominantly increased the levels of progestagens that are formed in the beginning of the steroidogenic pathway. The MA-10 Leydig cell assay shows added value over the widely used H295R steroidogenesis assay because of its fetal-like characteristics and specificity for the physiologically more relevant testicular Δ4 steroidogenic pathway. Therefore, adding an in vitro assay covering fetal testicular steroidogenesis, such as the MA-10 cell line, to the panel of tests used to screen potential endocrine disruptors, is highly recommendable.
(Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: (Fetal-like) steroidogenesis; (Structural) bisphenol analogues (TBBPA, BPA, BPF, BPS); Androgen receptor (AR); Glucocorticoid receptor (GR); Murine MA-10 Leydig cells; Testosterone (T)
المشرفين على المادة: 0 (Benzhydryl Compounds)
0 (Endocrine Disruptors)
0 (Phenols)
0 (Polybrominated Biphenyls)
0 (Receptors, Androgen)
0 (Receptors, Glucocorticoid)
0 (Sulfones)
3XMK78S47O (Testosterone)
80-09-1 (bis(4-hydroxyphenyl)sulfone)
FQI02RFC3A (tetrabromobisphenol A)
MLT3645I99 (bisphenol A)
تواريخ الأحداث: Date Created: 20150111 Date Completed: 20150413 Latest Revision: 20181202
رمز التحديث: 20231215
DOI: 10.1016/j.tox.2015.01.003
PMID: 25576683
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-3185
DOI:10.1016/j.tox.2015.01.003