دورية أكاديمية
أعرض في EDS

Biphasic role of calcium in mouse sperm capacitation signaling pathways.

التفاصيل البيبلوغرافية
العنوان: Biphasic role of calcium in mouse sperm capacitation signaling pathways.
المؤلفون: Navarrete FA; Department of Veterinary and Animal Science, Integrated Sciences Building, University of Massachusetts, Amherst MA, USA., García-Vázquez FA; Department of Veterinary and Animal Science, Integrated Sciences Building, University of Massachusetts, Amherst MA, USA.; Department of Physiology, Veterinary School, University of Murcia, Murcia, Spain.; International Excellence Campus for Higher Education and Research (Campus Mare Nostrum) and Institute for Biomedical Research of Murcia, Murcia, Spain., Alvau A; Department of Veterinary and Animal Science, Integrated Sciences Building, University of Massachusetts, Amherst MA, USA., Escoffier J; Department of Veterinary and Animal Science, Integrated Sciences Building, University of Massachusetts, Amherst MA, USA., Krapf D; Instituto de Biología Celular y Molecular de Rosario (CONICET), UNR, Buenos Aires, Argentina., Sánchez-Cárdenas C; Departamento de Genética del Desarrollo y Fisiología Molecular, IBT-UNAM, Cuernavaca, México., Salicioni AM; Department of Veterinary and Animal Science, Integrated Sciences Building, University of Massachusetts, Amherst MA, USA., Darszon A; Departamento de Genética del Desarrollo y Fisiología Molecular, IBT-UNAM, Cuernavaca, México., Visconti PE; Department of Veterinary and Animal Science, Integrated Sciences Building, University of Massachusetts, Amherst MA, USA.
المصدر: Journal of cellular physiology [J Cell Physiol] 2015 Aug; Vol. 230 (8), pp. 1758-1769.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Wiley-Liss Country of Publication: United States NLM ID: 0050222 Publication Model: Print Cited Medium: Internet ISSN: 1097-4652 (Electronic) Linking ISSN: 00219541 NLM ISO Abbreviation: J Cell Physiol Subsets: MEDLINE
أسماء مطبوعة: Publication: New York, NY : Wiley-Liss
Original Publication: Philadelphia, Wistar Institute of Anatomy and Biology.
مواضيع طبية MeSH: Calcium/*metabolism , Signal Transduction/*physiology , Sperm Capacitation/*physiology, Animals ; Blotting, Western ; Calcium Channels/metabolism ; Cyclic AMP/metabolism ; Male ; Mice ; Mice, Knockout ; Phosphorylation ; Sperm Motility/physiology ; Tyrosine/metabolism
مستخلص: Mammalian sperm acquire fertilizing ability in the female tract in a process known as capacitation. At the molecular level, capacitation is associated with up-regulation of a cAMP-dependent pathway, changes in intracellular pH, intracellular Ca(2+), and an increase in tyrosine phosphorylation. How these signaling systems interact during capacitation is not well understood. Results presented in this study indicate that Ca(2+) ions have a biphasic role in the regulation of cAMP-dependent signaling. Media without added Ca(2+) salts (nominal zero Ca(2+)) still contain micromolar concentrations of this ion. Sperm incubated in this medium did not undergo PKA activation or the increase in tyrosine phosphorylation suggesting that these phosphorylation pathways require Ca(2+). However, chelation of the extracellular Ca(2+) traces by EGTA induced both cAMP-dependent phosphorylation and the increase in tyrosine phosphorylation. The EGTA effect in nominal zero Ca(2+) media was mimicked by two calmodulin antagonists, W7 and calmidazolium, and by the calcineurin inhibitor cyclosporine A. These results suggest that Ca(2+) ions regulate sperm cAMP and tyrosine phosphorylation pathways in a biphasic manner and that some of its effects are mediated by calmodulin. Interestingly, contrary to wild-type mouse sperm, sperm from CatSper1 KO mice underwent PKA activation and an increase in tyrosine phosphorylation upon incubation in nominal zero Ca(2+) media. Therefore, sperm lacking Catsper Ca(2+) channels behave as wild-type sperm incubated in the presence of EGTA. This latter result suggests that Catsper transports the Ca(2+) involved in the regulation of cAMP-dependent and tyrosine phosphorylation pathways required for sperm capacitation.
(© 2015 Wiley Periodicals, Inc.)
References: Cell. 2014 May 8;157(4):808-22. (PMID: 24813608)
J Biol Chem. 2013 Dec 6;288(49):35307-20. (PMID: 24129574)
J Biol Chem. 2000 Mar 17;275(11):7994-9. (PMID: 10713118)
Development. 2000 Jun;127(11):2407-20. (PMID: 10804182)
Nature. 2001 Oct 11;413(6856):603-9. (PMID: 11595941)
Proc Natl Acad Sci U S A. 2001 Oct 23;98(22):12527-31. (PMID: 11675491)
Life Sci. 2003 Feb 21;72(14):1617-25. (PMID: 12551750)
Development. 2003 Apr;130(7):1317-26. (PMID: 12588848)
J Biol Chem. 2003 May 2;278(18):15922-6. (PMID: 12609998)
Proc Natl Acad Sci U S A. 2003 Sep 16;100(19):10676-81. (PMID: 12958208)
Nat Cell Biol. 2003 Dec;5(12):1117-22. (PMID: 14634667)
Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):14864-8. (PMID: 14657352)
Proc Natl Acad Sci U S A. 2014 Mar 11;111(10):3727-32. (PMID: 24567411)
Mol Reprod Dev. 2004 Mar;67(3):337-52. (PMID: 14735495)
J Biol Chem. 2004 Aug 6;279(32):33742-50. (PMID: 15178683)
Proc Natl Acad Sci U S A. 2004 Sep 14;101(37):13483-8. (PMID: 15340140)
Nature. 1970 Aug 15;227(5259):680-5. (PMID: 5432063)
Proc Natl Acad Sci U S A. 1980 Mar;77(3):1546-50. (PMID: 6445558)
J Cell Biol. 1988 May;106(5):1625-33. (PMID: 2836436)
J Biol Chem. 1989 Mar 25;264(9):5104-11. (PMID: 2538474)
Mol Reprod Dev. 1990 May;26(1):63-8. (PMID: 2112006)
Cell. 1991 Aug 23;66(4):807-15. (PMID: 1715244)
Development. 1994 Nov;120(11):3313-23. (PMID: 7720569)
Development. 1995 Apr;121(4):1129-37. (PMID: 7743926)
Development. 1995 Apr;121(4):1139-50. (PMID: 7538069)
Dev Biol. 1995 Oct;171(2):554-63. (PMID: 7556936)
Dev Biol. 1996 Feb 1;173(2):510-20. (PMID: 8606009)
Zygote. 1994 Nov;2(4):371-2. (PMID: 8665172)
Mol Reprod Dev. 1996 Oct;45(2):212-7. (PMID: 8914079)
Dev Biol. 1996 Nov 25;180(1):284-96. (PMID: 8948591)
Nature. 1951 Oct 20;168(4277):697-8. (PMID: 14882325)
Perspect Biol Med. 1959;3:44-54. (PMID: 13795187)
Nat Struct Mol Biol. 2005 Jan;12(1):32-7. (PMID: 15619637)
J Cell Sci. 2005 May 1;118(Pt 9):2013-22. (PMID: 15840651)
Mol Reprod Dev. 2005 Aug;71(4):495-508. (PMID: 15856425)
Dev Cell. 2005 Aug;9(2):249-59. (PMID: 16054031)
Biol Reprod. 2005 Sep;73(3):519-26. (PMID: 15878888)
J Biol Chem. 2005 Sep 16;280(37):32238-44. (PMID: 16036917)
Dev Biol. 2006 Aug 15;296(2):353-62. (PMID: 16842770)
Proc Natl Acad Sci U S A. 2007 Jan 23;104(4):1219-23. (PMID: 17227845)
J Biol Chem. 2008 Dec 19;283(51):35539-50. (PMID: 18957426)
Proc Natl Acad Sci U S A. 2008 Dec 30;105(52):20740-5. (PMID: 19074277)
Proc Natl Acad Sci U S A. 2009 Jan 20;106(3):667-8. (PMID: 19144927)
FEBS Lett. 2010 Mar 5;584(5):1041-6. (PMID: 20138882)
J Biol Chem. 2010 Mar 12;285(11):7977-85. (PMID: 20068039)
Physiology (Bethesda). 2010 Jun;25(3):165-75. (PMID: 20551230)
Asian J Androl. 2011 May;13(3):395-405. (PMID: 21540868)
Biol Reprod. 2011 Jun;84(6):1207-15. (PMID: 21349820)
J Cell Sci. 2012 Jan 15;125(Pt 2):473-85. (PMID: 22302997)
Expert Rev Proteomics. 2012 Oct;9(5):533-48. (PMID: 23194270)
J Biol Chem. 2012 Dec 28;287(53):44384-93. (PMID: 23095755)
Biol Reprod. 2013 Jun;88(6):138. (PMID: 23595906)
Proc Natl Acad Sci U S A. 2013 Nov 12;110(46):18543-8. (PMID: 24128762)
Mol Hum Reprod. 2014 Nov;20(11):1054-66. (PMID: 25180269)
معلومات مُعتمدة: R01 HD038082 United States HD NICHD NIH HHS; R01 HD044044 United States HD NICHD NIH HHS; HD38082 United States HD NICHD NIH HHS; HD44044 United States HD NICHD NIH HHS
المشرفين على المادة: 0 (Calcium Channels)
0 (Catsper1 protein, mouse)
42HK56048U (Tyrosine)
E0399OZS9N (Cyclic AMP)
SY7Q814VUP (Calcium)
تواريخ الأحداث: Date Created: 20150120 Date Completed: 20150715 Latest Revision: 20220408
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC4752735
DOI: 10.1002/jcp.24873
PMID: 25597298
قاعدة البيانات: MEDLINE
الوصف
تدمد:1097-4652
DOI:10.1002/jcp.24873