دورية أكاديمية
Twist1 and AP-1 cooperatively upregulate integrin α5 expression to induce invasion and the epithelial-mesenchymal transition.
| العنوان: | Twist1 and AP-1 cooperatively upregulate integrin α5 expression to induce invasion and the epithelial-mesenchymal transition. |
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| المؤلفون: | Nam EH; Immunotherapy Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejon, Korea., Lee Y; Immunotherapy Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejon, Korea, Department of Chemistry, Korea Advanced Institute of Science and Technology, Daejon, Korea., Moon B; Immunotherapy Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejon, Korea, Department of Functional Genomics, Korea University of Science and Technology, Daejon, Korea and., Lee JW; Department of Pharmacy, College of Pharmacy, Seoul National University, Seoul, Korea., Kim S; Immunotherapy Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejon, Korea, Department of Chemistry, Korea Advanced Institute of Science and Technology, Daejon, Korea, Department of Functional Genomics, Korea University of Science and Technology, Daejon, Korea and semikim@kribb.re.kr. |
| المصدر: | Carcinogenesis [Carcinogenesis] 2015 Mar; Vol. 36 (3), pp. 327-37. Date of Electronic Publication: 2015 Jan 18. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Irl Press At Oxford University Press Country of Publication: England NLM ID: 8008055 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1460-2180 (Electronic) Linking ISSN: 01433334 NLM ISO Abbreviation: Carcinogenesis Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oxford : Irl Press At Oxford University Press Original Publication: [New York, IRL Press] |
| مواضيع طبية MeSH: | Colorectal Neoplasms/*genetics , Epithelial-Mesenchymal Transition/*genetics , Integrin alpha5/*genetics , Nuclear Proteins/*metabolism , Transcription Factor AP-1/*metabolism , Twist-Related Protein 1/*metabolism, Activating Transcription Factor 2/genetics ; Activating Transcription Factor 2/metabolism ; Cell Line, Tumor ; Colorectal Neoplasms/pathology ; Gene Expression Regulation, Neoplastic ; Gene Knockdown Techniques ; Humans ; Integrin alpha5/metabolism ; JNK Mitogen-Activated Protein Kinases/genetics ; JNK Mitogen-Activated Protein Kinases/metabolism ; Nuclear Proteins/genetics ; Promoter Regions, Genetic ; Transcription Factor AP-1/genetics ; Twist-Related Protein 1/genetics ; Up-Regulation |
| مستخلص: | Epithelial-mesenchymal transition (EMT) is an important process implicated in tumor invasion and metastasis. Twist1 is a transcription factor that induces EMT, including E-cadherin suppression and cancer cell migration and invasion; hence it promotes cancer metastasis. Twist1 directly or indirectly regulates the expression of various genes and cellular functions involved in cancer progression. However, the underlying mechanisms remain largely unknown. In this study, we investigated the molecular basis for Twist1-mediated invasion and EMT. In human cancer cells, Twist1 was found to directly upregulate transcription of the mesenchymal gene integrin α5 in an E-box-independent, but activating protein-1 (AP-1) element-dependent, manner. Twist1 activated the integrin α5 promoter by interacting with and activating the transcription factor AP-1, composed of c-Jun and activating transcription factor-2 (ATF-2); it also enhanced the nuclear presence of ATF-2. AP-1 was critical for Twist1-induced cancer cell invasion, primarily through the induction of integrin α5, which activated c-Jun N-terminal kinase and focal adhesion kinase-signaling activities. Using data from The Cancer Genome Atlas, we found that Twist1 expression positively correlates with integrin α5 expression in human colorectal cancers. These findings suggest that cooperation between Twist1 and AP-1 represents a novel mechanism for EMT and tumor invasiveness. This study supports further investigation into the molecular basis underlying the diverse Twist1-mediated functions that occur during tumor progression. (© The Author 2015. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.) |
| المشرفين على المادة: | 0 (ATF2 protein, human) 0 (Activating Transcription Factor 2) 0 (Integrin alpha5) 0 (Nuclear Proteins) 0 (TWIST1 protein, human) 0 (Transcription Factor AP-1) 0 (Twist-Related Protein 1) EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases) |
| تواريخ الأحداث: | Date Created: 20150121 Date Completed: 20150624 Latest Revision: 20220321 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1093/carcin/bgv005 |
| PMID: | 25600770 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1460-2180 |
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| DOI: | 10.1093/carcin/bgv005 |