دورية أكاديمية
A trans-dominant form of Gag restricts Ty1 retrotransposition and mediates copy number control.
| العنوان: | A trans-dominant form of Gag restricts Ty1 retrotransposition and mediates copy number control. |
|---|---|
| المؤلفون: | Saha A; Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA., Mitchell JA; Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA., Nishida Y; Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA., Hildreth JE; Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA., Ariberre JA; Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA., Gilbert WV; Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA., Garfinkel DJ; Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA djgarf@bmb.uga.edu. |
| المصدر: | Journal of virology [J Virol] 2015 Apr; Vol. 89 (7), pp. 3922-38. Date of Electronic Publication: 2015 Jan 21. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society For Microbiology Country of Publication: United States NLM ID: 0113724 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-5514 (Electronic) Linking ISSN: 0022538X NLM ISO Abbreviation: J Virol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Washington Dc : American Society For Microbiology Original Publication: Baltimore, American Society for Microbiology. |
| مواضيع طبية MeSH: | Recombination, Genetic* , Retroelements*, Saccharomyces cerevisiae/*genetics , gag Gene Products, Human Immunodeficiency Virus/*metabolism, Gene Expression ; Protein Interaction Mapping ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism ; Virosomes/metabolism ; Virus Assembly ; gag Gene Products, Human Immunodeficiency Virus/genetics |
| مستخلص: | Unlabelled: Saccharomyces cerevisiae and Saccharomyces paradoxus lack the conserved RNA interference pathway and utilize a novel form of copy number control (CNC) to inhibit Ty1 retrotransposition. Although noncoding transcripts have been implicated in CNC, here we present evidence that a truncated form of the Gag capsid protein (p22) or its processed form (p18) is necessary and sufficient for CNC and likely encoded by Ty1 internal transcripts. Coexpression of p22/p18 and Ty1 decreases mobility more than 30,000-fold. p22/p18 cofractionates with Ty1 virus-like particles (VLPs) and affects VLP yield, protein composition, and morphology. Although p22/p18 and Gag colocalize in the cytoplasm, p22/p18 disrupts sites used for VLP assembly. Glutathione S-transferase (GST) affinity pulldowns also suggest that p18 and Gag interact. Therefore, this intrinsic Gag-like restriction factor confers CNC by interfering with VLP assembly and function and expands the strategies used to limit retroelement propagation. Importance: Retrotransposons dominate the chromosomal landscape in many eukaryotes, can cause mutations by insertion or genome rearrangement, and are evolutionarily related to retroviruses such as HIV. Thus, understanding factors that limit transposition and retroviral replication is fundamentally important. The present work describes a retrotransposon-encoded restriction protein derived from the capsid gene of the yeast Ty1 element that disrupts virus-like particle assembly in a dose-dependent manner. This form of copy number control acts as a molecular rheostat, allowing high levels of retrotransposition when few Ty1 elements are present and inhibiting transposition as copy number increases. Thus, yeast and Ty1 have coevolved a form of copy number control that is beneficial to both "host and parasite." To our knowledge, this is the first Gag-like retrotransposon restriction factor described in the literature and expands the ways in which restriction proteins modulate retroelement replication. (Copyright © 2015, American Society for Microbiology. All Rights Reserved.) |
| التعليقات: | Erratum in: J Virol. 2016 Apr 29;90(10 ):5210. (PMID: 27130940) |
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| معلومات مُعتمدة: | K99 GM081399 United States GM NIGMS NIH HHS; GM095622 United States GM NIGMS NIH HHS; R01 GM095622 United States GM NIGMS NIH HHS; R01 GM094303 United States GM NIGMS NIH HHS; R00 GM081399 United States GM NIGMS NIH HHS; F32 GM112474 United States GM NIGMS NIH HHS; 1F32GM112474-01 United States GM NIGMS NIH HHS |
| المشرفين على المادة: | 0 (Recombinant Proteins) 0 (Retroelements) 0 (Virosomes) 0 (gag Gene Products, Human Immunodeficiency Virus) |
| تواريخ الأحداث: | Date Created: 20150123 Date Completed: 20150521 Latest Revision: 20240322 |
| رمز التحديث: | 20240322 |
| مُعرف محوري في PubMed: | PMC4403431 |
| DOI: | 10.1128/JVI.03060-14 |
| PMID: | 25609815 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1098-5514 |
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| DOI: | 10.1128/JVI.03060-14 |