دورية أكاديمية
أعرض في EDS

Identifying the similarities and differences between single nucleotide polymorphism array (SNPa) analysis and karyotyping in acute myeloid leukemia and myelodysplastic syndromes.

التفاصيل البيبلوغرافية
العنوان: Identifying the similarities and differences between single nucleotide polymorphism array (SNPa) analysis and karyotyping in acute myeloid leukemia and myelodysplastic syndromes.
المؤلفون: Noronha TR; Universidade Federal de São Paulo (UNIFESP), São Paulo, SP, Brazil. Electronic address: thinoronha@yahoo.com.br., Rohr SS; Universidade Federal de São Paulo (UNIFESP), São Paulo, SP, Brazil., Chauffaille Mde L; Universidade Federal de São Paulo (UNIFESP), São Paulo, SP, Brazil; Grupo Fleury, São Paulo, Brazil.
المصدر: Revista brasileira de hematologia e hemoterapia [Rev Bras Hematol Hemoter] 2015 Jan-Feb; Vol. 37 (1), pp. 48-54. Date of Electronic Publication: 2014 Nov 21.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Brazil NLM ID: 101220159 Publication Model: Print-Electronic Cited Medium: Print ISSN: 1516-8484 (Print) Linking ISSN: 15168484 NLM ISO Abbreviation: Rev Bras Hematol Hemoter Subsets: PubMed not MEDLINE
أسماء مطبوعة: Publication: 2014- : [Rio de Janeiro, Brazil] : Elsevier
Original Publication: Rio de Janeiro : Sociedade Brasileira de Hematologia e Hemoterapia
مستخلص: Objective: To standardize the single nucleotide polymorphism array (SNPa) method in acute myeloid leukemia/myelodysplastic syndromes, and to identify the similarities and differences between the results of this method and karyotyping.
Methods: Twenty-two patients diagnosed with acute myeloid leukemia and three with myelodysplastic syndromes were studied. The G-banding karyotyping and single nucleotide polymorphism array analysis (CytoScan(®) HD) were performed using cells from bone marrow, DNA extracted from mononuclear cells from bone marrow and buccal cells (BC).
Results: The mean age of the patients studied was 54 years old, and the median age was 55 years (range: 28-93). Twelve (48%) were male and 13 (52%) female. Ten patients showed abnormal karyotypes (40.0%), 11 normal (44.0%) and four had no mitosis (16.0%). Regarding the results of bone marrow single nucleotide polymorphism array analysis: 17 were abnormal (68.0%) and eight were normal (32.0%). Comparing the two methods, karyotyping identified a total of 17 alterations (8 deletions/losses, 7 trissomies/gains, and 2 translocations) and single nucleotide polymorphism array analysis identified a total of 42 alterations (17 losses, 16 gains and 9 copy-neutral loss of heterozygosity).
Conclusion: It is possible to standardize single nucleotide polymorphism array analysis in acute myeloid leukemia/myelodysplastic syndromes and compare the results with the abnormalities detected by karyotyping. Single nucleotide polymorphism array analysis increased the detection rate of abnormalities compared to karyotyping and also identified a new set of abnormalities that deserve further investigation in future studies.
(Copyright © 2014 Associação Brasileira de Hematologia, Hemoterapia e Terapia Celular. Published by Elsevier Editora Ltda. All rights reserved.)
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فهرسة مساهمة: Keywords: Acute myeloid leukemia; Karyotype; Loss of heterozygosity; Myelodysplastic syndromes; Single nucleotide polymorphism
تواريخ الأحداث: Date Created: 20150202 Date Completed: 20150202 Latest Revision: 20200930
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC4318843
DOI: 10.1016/j.bjhh.2014.09.011
PMID: 25638768
قاعدة البيانات: MEDLINE
الوصف
تدمد:1516-8484
DOI:10.1016/j.bjhh.2014.09.011