دورية أكاديمية
Ecology and genomic features of infection with Mycobacterium avium subspecies paratuberculosis in Egypt.
| العنوان: | Ecology and genomic features of infection with Mycobacterium avium subspecies paratuberculosis in Egypt. |
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| المؤلفون: | Amin AS; Biotechnology Research Unit, Animal Reproduction Research Institute (ARRI), Giza, Egypt., Hsu CY; Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, WI, USA., Darwish SF; Biotechnology Research Unit, Animal Reproduction Research Institute (ARRI), Giza, Egypt., Ghosh P; Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, WI, USA., AbdEl-Fatah EM; Biotechnology Research Unit, Animal Reproduction Research Institute (ARRI), Giza, Egypt., Behour TS; Biotechnology Research Unit, Animal Reproduction Research Institute (ARRI), Giza, Egypt., Talaat AM; Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, WI, USA Department of Food Hygiene and Control, Faculty of Veterinary Medicine Cairo University, Giza, Egypt atalaat@wisc.edu. |
| المصدر: | Microbiology (Reading, England) [Microbiology (Reading)] 2015 Apr; Vol. 161 (Pt 4), pp. 807-18. Date of Electronic Publication: 2015 Feb 08. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Microbiology Society Country of Publication: England NLM ID: 9430468 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1465-2080 (Electronic) Linking ISSN: 13500872 NLM ISO Abbreviation: Microbiology (Reading) Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : London : Microbiology Society Original Publication: Reading, U.K. : Society for General Microbiology, c1994- |
| مواضيع طبية MeSH: | Genomics*, Mycobacterium avium subsp. paratuberculosis/*genetics , Paratuberculosis/*microbiology, Animals ; Cattle ; Ecology ; Egypt/epidemiology ; Genome ; Genome, Bacterial ; Mice ; Molecular Sequence Data ; Mycobacterium avium subsp. paratuberculosis/isolation & purification ; Paratuberculosis/epidemiology ; Polymorphism, Single Nucleotide ; Prevalence ; Virulence/genetics |
| مستخلص: | Mycobacterium avium subspecies paratuberculosis (M. paratuberculosis) is the causative agent of paratuberculosis, or Johne's disease, in cattle, with potential involvement in cases of Crohn's disease in humans. Johne's disease is found worldwide and is economically important for both beef and dairy industries. In an effort to characterize this important infection in Egypt, we analysed the ecological and genomic features of recent isolates of M. paratuberculosis. In this report, we examined 26 Holstein dairy herds distributed throughout Egypt, from 2010 to 2013. Using PCR analysis of faecal samples, we estimated a mean herd-level prevalence of 65.4 %, with animal-level infection that reached a mean of 13.6 % among animals suffering from diarrhoea. Whole genome sequencing of field isolates identified numerous single nucleotide polymorphisms among field isolates relative to the standard M. paratuberculosis K10 genome. Interestingly, the virulence of M. paratuberculosis isolates from Egypt revealed diverse virulence phenotypes in the murine model of paratuberculosis, with significant differences in tissue colonization, particularly during the chronic stage of infection. Overall, our analysis confirmed that Johne's disease is a newly identified problem in Egypt and indicated that M. paratuberculosis has potentially diverse genotypes that impact its virulence. Further ecological mapping and genomic analysis of M. paratuberculosis will enhance our understanding of the transmission and evolutionary dynamics of this pathogen under natural field conditions. (© 2015 The Authors.) |
| سلسلة جزيئية: | GENBANK CP010113; CP010114 |
| تواريخ الأحداث: | Date Created: 20150211 Date Completed: 20160314 Latest Revision: 20200826 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1099/mic.0.000051 |
| PMID: | 25667007 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1465-2080 |
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| DOI: | 10.1099/mic.0.000051 |