دورية أكاديمية
أعرض في EDS

Follow-up of high-risk HPV positive women by combined cytology and bi-marker CADM1/MAL methylation analysis on cervical scrapes.

التفاصيل البيبلوغرافية
العنوان: Follow-up of high-risk HPV positive women by combined cytology and bi-marker CADM1/MAL methylation analysis on cervical scrapes.
المؤلفون: Verhoef VM; Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands., van Kemenade FJ; Department of Pathology, Erasmus MC University Medical Center, Rotterdam, The Netherlands., Rozendaal L; Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands., Heideman DA; Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands., Bosgraaf RP; Department of Obstetrics and Gynaecology, Radboud University Medical Center, Nijmegen, The Netherlands., Hesselink AT; Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands., Melchers WJ; Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, The Netherlands., Massuger LF; Department of Obstetrics and Gynaecology, Radboud University Medical Center, Nijmegen, The Netherlands., Bekkers RL; Department of Obstetrics and Gynaecology, Radboud University Medical Center, Nijmegen, The Netherlands., Steenbergen RD; Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands., Berkhof J; Department of Epidemiology and Biostatistics, VU University Medical Center, Amsterdam, The Netherlands., Snijders PJ; Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands., Meijer CJ; Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands. Electronic address: cjlm.meijer@vumc.nl.
المصدر: Gynecologic oncology [Gynecol Oncol] 2015 Apr; Vol. 137 (1), pp. 55-9. Date of Electronic Publication: 2015 Feb 08.
نوع المنشور: Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Academic Press Country of Publication: United States NLM ID: 0365304 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-6859 (Electronic) Linking ISSN: 00908258 NLM ISO Abbreviation: Gynecol Oncol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: New York, Academic Press.
مواضيع طبية MeSH: DNA Methylation*, Cell Adhesion Molecules/*genetics , Immunoglobulins/*genetics , Myelin and Lymphocyte-Associated Proteolipid Proteins/*genetics , Papillomavirus Infections/*genetics , Uterine Cervical Neoplasms/*genetics , Uterine Cervical Dysplasia/*genetics, Adult ; Alphapapillomavirus/isolation & purification ; Biomarkers, Tumor/genetics ; Cell Adhesion Molecule-1 ; Female ; Humans ; Middle Aged ; Papillomavirus Infections/pathology ; Risk Factors ; Triage/methods ; Uterine Cervical Neoplasms/pathology ; Uterine Cervical Neoplasms/virology ; Vaginal Smears/methods ; Uterine Cervical Dysplasia/pathology ; Uterine Cervical Dysplasia/virology
مستخلص: Objectives: Triage of HPV screen-positive women is needed to identify those with underlying cervical intraepithelial neoplasia grade 2/3 or worse (CIN2/3+). Presently, cytology on a physician-taken cervical scrape is mostly accepted as triage test, but needs follow-up testing in order not to miss severe disease. Here, we evaluated the performance of combined cytology and bi-marker CADM1/MAL-methylation analysis as triage test on physician-taken cervical scrapes of HPV positive women.
Methods: In this post-hoc analysis, we used 364 left-over HPV positive cytology triage samples of participants of a randomized controlled trial (PROHTECT-3: n=46,001) performed in population-based cervical screening. Study endpoints were CIN2+ and CIN3+ detection. Cytology testing with and without methylation marker analysis was evaluated with regard to sensitivity, specificity, positive and negative predictive value, and referral rate.
Results: Bi-marker CADM1/MAL-methylation positivity increased proportionally with severity of underlying lesions. Overall, cytology and bi-marker CADM1/MAL-methylation analysis yielded similar performances with regard to CIN3+ detection, yet in combination a significantly higher sensitivity for CIN3+ (88.7%) was obtained at a specificity of 53.6% and a colposcopy referral rate of 53.6%. The combined strategy detected all six cervical cancers, whereas triage by cytology alone failed to detect two of them.
Conclusions: Cytology and bi-marker CADM1/MAL-methylation analysis perform complementary for CIN2+/CIN3+ detection when used as triage tool on cervical scrapes of HPV positive women. This approach not only results in a higher CIN3+ sensitivity than cytology triage with an acceptable referral rate, but also seems to reduce the risk of missing cervical cancers and advanced high-grade lesions.
(Copyright © 2015 Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: Cervical intraepithelial neoplasia; Cervical scrape; Cervical screening; Cytology; DNA methylation; Human papillomavirus; Triage
المشرفين على المادة: 0 (Biomarkers, Tumor)
0 (CADM1 protein, human)
0 (Cell Adhesion Molecule-1)
0 (Cell Adhesion Molecules)
0 (Immunoglobulins)
0 (MAL protein, human)
0 (Myelin and Lymphocyte-Associated Proteolipid Proteins)
تواريخ الأحداث: Date Created: 20150211 Date Completed: 20150528 Latest Revision: 20221207
رمز التحديث: 20240628
DOI: 10.1016/j.ygyno.2015.01.550
PMID: 25667975
قاعدة البيانات: MEDLINE
الوصف
تدمد:1095-6859
DOI:10.1016/j.ygyno.2015.01.550