دورية أكاديمية
Dendritic cells induce specific cytotoxic T lymphocytes against prostate cancer TRAMP-C2 cells loaded with freeze- thaw antigen and PEP-3 peptide.
| العنوان: | Dendritic cells induce specific cytotoxic T lymphocytes against prostate cancer TRAMP-C2 cells loaded with freeze- thaw antigen and PEP-3 peptide. |
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| المؤلفون: | Liu XQ; Molecular Medicine and Cancer Research Center, Department of Biochemistry and Molecular Biology, Chongqing Medical University, Chongqing, China E-mail : fazicq@163.com., Jiang R, Li SQ, Wang J, Yi FP |
| المصدر: | Asian Pacific journal of cancer prevention : APJCP [Asian Pac J Cancer Prev] 2015; Vol. 16 (2), pp. 571-8. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Asian Pacific Organization for Cancer Prevention Country of Publication: Thailand NLM ID: 101130625 Publication Model: Print Cited Medium: Internet ISSN: 2476-762X (Electronic) Linking ISSN: 15137368 NLM ISO Abbreviation: Asian Pac J Cancer Prev Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Bangkok : Asian Pacific Organization for Cancer Prevention, |
| مواضيع طبية MeSH: | Freezing*, Antigens, Neoplasm/*immunology , Dendritic Cells/*immunology , Peptides/*immunology , Prostatic Neoplasms/*immunology , T-Lymphocytes, Cytotoxic/*immunology, Animals ; Antigens, Neoplasm/metabolism ; Apoptosis ; Blotting, Western ; Cell Movement ; Cell Proliferation ; Cells, Cultured ; Cytokines/metabolism ; Dendritic Cells/metabolism ; Dendritic Cells/pathology ; Flow Cytometry ; Humans ; Male ; Mice ; Mice, Inbred C57BL ; Peptides/metabolism ; Prostatic Neoplasms/metabolism ; Prostatic Neoplasms/pathology ; T-Lymphocytes, Cytotoxic/metabolism ; T-Lymphocytes, Cytotoxic/pathology ; Wound Healing ; Xenograft Model Antitumor Assays |
| مستخلص: | Prostate cancer is the most common cancer in men. In this study, we investigated immune responses of cytotoxic T lymphocytes (CTLs) against TRAMP-C2 prostate cancer cells after activation by dendritic cells (DCs) loaded with TRAMP-C2 freeze-thaw antigen and/or PEP-3 peptide in vitro. Bone marrow-derived DC from the bone marrow of the C57BL/6 were induced to mature by using the cytokine of rhGM-CSF and rhIL-4, and loaded with either the freeze-thaw antigen or PEP-3 peptide or both of them. Maturation of DCs was detected by flow cytometry. The killing efficiency of the CTLs on TRAMP-C2 cells were detected by flow cytometry, CCK8, colony formation, transwell migration, and wound-healing assay. The levels of the IFN-γ, TNF-β and IL-12 were measured by enzyme-linked immunosorbent assay (ELISA). Compared with the unloaded DCs, the loaded DCs had significantly increased expression of several phenotypes related to DC maturation. CTLs activated by DCs loaded with freeze-thaw antigen and PEP-3 peptide had more evident cytotoxicity against TRAMP-C2 cells in vitro. The secretion levels of IFN-γ, TNF-β and IL-12, secreted by DCs loaded with antigen and PEP-3 and interaction with T cells, were higher than in the other groups. Our results suggest that the CTLs activated by DCs loaded with TRAMP-C2 freeze-thaw antigen and PEP-3 peptide exert a remarkable killing efficiency against TRAMP-C2 cells in vitro. |
| المشرفين على المادة: | 0 (Antigens, Neoplasm) 0 (Cytokines) 0 (Pep-3 peptide) 0 (Peptides) |
| تواريخ الأحداث: | Date Created: 20150217 Date Completed: 20151203 Latest Revision: 20190606 |
| رمز التحديث: | 20221213 |
| DOI: | 10.7314/apjcp.2015.16.2.571 |
| PMID: | 25684489 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2476-762X |
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| DOI: | 10.7314/apjcp.2015.16.2.571 |