دورية أكاديمية
Genotoxicity of chemical compounds identification and assessment by yeast cells transformed with GFP reporter constructs regulated by the PLM2 or DIN7 promoter.
| العنوان: | Genotoxicity of chemical compounds identification and assessment by yeast cells transformed with GFP reporter constructs regulated by the PLM2 or DIN7 promoter. |
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| المؤلفون: | Bui VN; Institute of Biotechnology, Vietnam Academy of Science and Technology (VAST), Hanoi, Vietnam., Nguyen TT; Thai Nguyen University of Sciences, Thai Nguyen University, Thai Nguyen, Hanoi, Vietnam., Bettarel Y; Institute of Research and Development, UMR ECOSYM, Montpellier, France., Nguyen TH; Institute of Biotechnology, Vietnam Academy of Science and Technology (VAST), Hanoi, Vietnam., Pham TL; Institute of Biotechnology, Vietnam Academy of Science and Technology (VAST), Hanoi, Vietnam., Hoang TY; Institute of Biotechnology, Vietnam Academy of Science and Technology (VAST), Hanoi, Vietnam., Nguyen VT; Thai Nguyen University of Sciences, Thai Nguyen University, Thai Nguyen, Hanoi, Vietnam., Nghiem NM; Institute of Biotechnology, Vietnam Academy of Science and Technology (VAST), Hanoi, Vietnam., Wölfl S; Institute of Pharmacy and Molecular Biotechnology (IPMB), Heidelberg University, Heidelberg, Germany wolfl@uni-hd.de. |
| المصدر: | International journal of toxicology [Int J Toxicol] 2015 Jan-Feb; Vol. 34 (1), pp. 31-43. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Sage Publications Country of Publication: United States NLM ID: 9708436 Publication Model: Print Cited Medium: Internet ISSN: 1092-874X (Electronic) Linking ISSN: 10915818 NLM ISO Abbreviation: Int J Toxicol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2009- : Thousand Oaks, CA : Sage Publications Original Publication: Washington, DC : Taylor & Francis, c1997- |
| مواضيع طبية MeSH: | Genes, Reporter*, Exodeoxyribonucleases/*genetics , Green Fluorescent Proteins/*genetics , Mutagens/*toxicity , Saccharomyces cerevisiae/*genetics , Saccharomyces cerevisiae Proteins/*genetics, DNA Helicases/genetics ; DNA Repair Enzymes/genetics ; Green Fluorescent Proteins/metabolism ; Mutagenicity Tests ; Plasmids ; Promoter Regions, Genetic ; Ribonucleotide Reductases/genetics |
| مستخلص: | Yeast cells transformed with high-copy number plasmids comprising a green fluorescent protein (GFP)-encoding gene optimized for yeast under the control of the new DIN7 or PLM2 and the established RNR2 and RAD54 promoters were used to assess the genotoxic potential of chemical compounds. The activity of potential DNA-damaging agents was investigated by genotoxicity assays and by OxoPlate assay in the presence of various test compounds. The fluorescence signal generated by GFP in response to DNA damage was related to the different concentrations of analytes and the analyte-dependent GFP synthesis. The use of distinct DNA damage-inducible promoters presents alternative genotoxicity testing strategies by selective induction of promoters in response to DNA damage. The new DIN7 and PLM2 systems show higher sensitivity than the RNR2 and RAD54 systems in detecting 4-nitroquinoline-N-oxide and actinomycin D. Both DIN7 and PLM2 systems are able to detect camptothecin while RNR2 and RAD54 systems are not. Automated laboratory systems with assay performance on 384-well microplates provide for cost-effective high-throughput screening of DNA-damaging agents, reducing compound consumption to about 53% as compared with existing eukaryotic genotoxicity bioassays. (© The Author(s) 2015.) |
| فهرسة مساهمة: | Keywords: DNA damage-inducible promoters; DNA-damaging agents; GFP; biosensor; genotoxicity |
| المشرفين على المادة: | 0 (Mutagens) 0 (Saccharomyces cerevisiae Proteins) 147336-22-9 (Green Fluorescent Proteins) EC 1.17.4.- (RNR2 protein, S cerevisiae) EC 1.17.4.- (Ribonucleotide Reductases) EC 3.1.- (DIN7 protein, S cerevisiae) EC 3.1.- (Exodeoxyribonucleases) EC 3.6.1.- (RAD54 protein, S cerevisiae) EC 3.6.4.- (DNA Helicases) EC 6.5.1.- (DNA Repair Enzymes) |
| تواريخ الأحداث: | Date Created: 20150219 Date Completed: 20151029 Latest Revision: 20150218 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1177/1091581814566870 |
| PMID: | 25691521 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1092-874X |
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| DOI: | 10.1177/1091581814566870 |