دورية أكاديمية
[Immunoassay of nine serological tumor markers on hydrogel-based microchip].
| العنوان: | [Immunoassay of nine serological tumor markers on hydrogel-based microchip]. |
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| المؤلفون: | Zubcova ZhI, Savvateeva EN, Butvilovskaia VI, Cybul'skaia MV, Chechetkin VR, Samokhina LO, Vinnitskiĭ LI, Maslennikov VV, Reznikov IuP, Zasedatelev AS, Rubina AIu |
| المصدر: | Bioorganicheskaia khimiia [Bioorg Khim] 2013 Nov-Dec; Vol. 39 (6), pp. 693-704. |
| نوع المنشور: | Journal Article |
| اللغة: | Russian |
| بيانات الدورية: | Publisher: Nauka Country of Publication: Russia (Federation) NLM ID: 7804941 Publication Model: Print Cited Medium: Print ISSN: 0132-3423 (Print) Linking ISSN: 01323423 NLM ISO Abbreviation: Bioorg Khim Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Moskva, Nauka |
| مواضيع طبية MeSH: | Protein Array Analysis*, Biomarkers, Tumor/*blood , Colorectal Neoplasms/*blood , Neoplasm Proteins/*blood, Adult ; Aged ; Antibodies/chemistry ; Antibodies/immunology ; Female ; Humans ; Hydrogel, Polyethylene Glycol Dimethacrylate ; Immunoassay ; Male ; Middle Aged |
| مستخلص: | A prototype of test-system for simultaneous quantitative assay of nine tumor markers in blood serum was developed. The main constituent of the test-system is OM-9 biochip containing immobilized antibodies against nine oncomarkers: α-fetoprotein (AFP), carcinoembryonic antigen (CEA), human chorionic gonadotropin (HCG), cancer antigen 15-3 (CA 15-3), cancer antigen 125 (CA 125), cancer antigen 19-9 (CA 19-9), prostate-specific antigen, total (PSAtot) and free (PSAfree) forms, neuron-specific enolase (NSE). The biochip-based assay procedure for carrying out simultaneous quantitative determination of nine tumor markers in patient's blood serum: two-steps sandwich-immunoassay, was proposed. The main analytical characteristics of the method were obtained. The results permit to consider the prototype of the test-system as a promising instrument for clinical application. The test-system prototype was tested using blood serum samples of oncological patients (252 samples) and healthy donors (185 samples). Increased concentrations of one or more tumor markers above the normal level were found in 76.6% cases of oncological patients and only in 6% cases of healthy donors. For colorectal cancer patients group, application of modern statistical methods of data-processing in medical researchers, i.e. ROC-analysis and logistic regression, indicted that the simultaneous assay of nine tumor markers on biochips showed much more diagnostic significance (area under the ROC-curve (AUC) reached 0.84) than traditional assay of 2 tumor markers, CEA and CA 19-9 (AUC = 0.59). The developed biochip-based test-system can be recommended both for the estimation of people's health, e.g., for standard medical examination, and for tracking of tumoral process in postsurgical period or after specific tumor treatment. |
| المشرفين على المادة: | 0 (Antibodies) 0 (Biomarkers, Tumor) 0 (Neoplasm Proteins) 25852-47-5 (Hydrogel, Polyethylene Glycol Dimethacrylate) |
| تواريخ الأحداث: | Date Created: 20150221 Date Completed: 20150407 Latest Revision: 20171116 |
| رمز التحديث: | 20221213 |
| PMID: | 25696931 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0132-3423 |
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