دورية أكاديمية
The galactose-binding lectin isolated from Bauhinia bauhinioides Mart seeds inhibits neutrophil rolling and adhesion via primary cytokines.
| العنوان: | The galactose-binding lectin isolated from Bauhinia bauhinioides Mart seeds inhibits neutrophil rolling and adhesion via primary cytokines. |
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| المؤلفون: | Girão DK; Departamento de Morfologia, Universidade Federal do Ceará, Rua Delmiro de Farias s/n, 60430-170, Fortaleza, Ceará, Brazil., Cavada BS, de Freitas Pires A, Martins TV, Franco ÁX, Morais CM, Santiago do Nascimento K, Delatorre P, da Silva HC, Nagano CS, Assreuy AM, Soares PM |
| المصدر: | Journal of molecular recognition : JMR [J Mol Recognit] 2015 May; Vol. 28 (5), pp. 285-92. Date of Electronic Publication: 2015 Feb 23. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: John Wiley & Sons Country of Publication: England NLM ID: 9004580 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1099-1352 (Electronic) Linking ISSN: 09523499 NLM ISO Abbreviation: J Mol Recognit Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Original Publication: London, UK : Heydon & Son, c1988- |
| مواضيع طبية MeSH: | Anti-Inflammatory Agents/*pharmacology , Bauhinia/*chemistry , Galectins/*pharmacology , Neutrophils/*physiology , Plant Extracts/*pharmacology , Plant Lectins/*pharmacology, Amino Acid Sequence ; Animals ; Anti-Inflammatory Agents/chemistry ; Cell Adhesion ; Cytokines/physiology ; Drug Evaluation, Preclinical ; Galectins/chemistry ; Leukocyte Rolling ; Molecular Sequence Data ; Neutrophils/drug effects ; Peritonitis/immunology ; Plant Extracts/chemistry ; Plant Lectins/chemistry ; Rats, Wistar ; Seeds/chemistry |
| مستخلص: | In this study, the amino acid sequence and anti-inflammatory effect of Bauhinia bauhinioides (BBL) lectin were evaluated. Tandem mass spectrometry revealed that BBL possesses 86 amino acid residues. BBL (1 mg/kg) intravenously injected in rats 30 min prior to inflammatory stimuli inhibited the cellular edema induced by carrageenan in only the second phase (21% - 3 h, 19% - 4 h) and did not alter the osmotic edema induced by dextran. BBL also inhibited carrageenan peritoneal neutrophil migration (51%), leukocyte rolling (58%) and adhesion (68%) and the neutrophil migration induced by TNF-α (64%). These effects were reversed by the association of BBL with galactose, demonstrating that the carbohydrate-binding domain is essential for lectin activity. In addition, BBL reduced myeloperoxidase activity (84%) and TNF-α (68%) and IL1-β (47%) levels. In conclusion, the present investigation demonstrated that BBL contains highly homologous isolectins, resulting in a total of 86 amino acid residues, and exhibits anti-inflammatory activity by inhibiting neutrophil migration by reducing TNF-α and IL1-β levels via the lectin domain. (Copyright © 2015 John Wiley & Sons, Ltd.) |
| فهرسة مساهمة: | Keywords: Bauhinia bauhinioides; TNF-α; anti-inflammatory effect; galactose-binding lectin; neutrophil |
| المشرفين على المادة: | 0 (Anti-Inflammatory Agents) 0 (Cytokines) 0 (Galectins) 0 (Plant Extracts) 0 (Plant Lectins) |
| تواريخ الأحداث: | Date Created: 20150224 Date Completed: 20160119 Latest Revision: 20150415 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1002/jmr.2441 |
| PMID: | 25706245 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1099-1352 |
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| DOI: | 10.1002/jmr.2441 |