دورية أكاديمية
Activity-driven relaxation of the cortical actomyosin II network synchronizes Munc18-1-dependent neurosecretory vesicle docking.
| العنوان: | Activity-driven relaxation of the cortical actomyosin II network synchronizes Munc18-1-dependent neurosecretory vesicle docking. |
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| المؤلفون: | Papadopulos A; The Clem Jones Centre for Ageing Dementia Research, Queensland Brain Institute, The University of Queensland, Brisbane, Queensland 4072, Australia., Gomez GA; Division of Molecular Cell Biology Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland 4072, Australia., Martin S; The Clem Jones Centre for Ageing Dementia Research, Queensland Brain Institute, The University of Queensland, Brisbane, Queensland 4072, Australia., Jackson J; Molecular and Cellular Neuroscience Laboratory, Department of Human Physiology, Flinders University, Adelaide, South Australia 5159, Australia., Gormal RS; The Clem Jones Centre for Ageing Dementia Research, Queensland Brain Institute, The University of Queensland, Brisbane, Queensland 4072, Australia., Keating DJ; Molecular and Cellular Neuroscience Laboratory, Department of Human Physiology, Flinders University, Adelaide, South Australia 5159, Australia., Yap AS; Division of Molecular Cell Biology Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland 4072, Australia., Meunier FA; The Clem Jones Centre for Ageing Dementia Research, Queensland Brain Institute, The University of Queensland, Brisbane, Queensland 4072, Australia. |
| المصدر: | Nature communications [Nat Commun] 2015 Feb 24; Vol. 6, pp. 6297. Date of Electronic Publication: 2015 Feb 24. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [London] : Nature Pub. Group |
| مواضيع طبية MeSH: | Neurosecretion*, Actins/*metabolism , Chromaffin Cells/*physiology , Munc18 Proteins/*metabolism , Myosin Type II/*metabolism , Secretory Vesicles/*physiology, Animals ; Cattle ; Heterocyclic Compounds, 4 or More Rings ; PC12 Cells ; Rats |
| مستخلص: | In neurosecretory cells, secretory vesicles (SVs) undergo Ca(2+)-dependent fusion with the plasma membrane to release neurotransmitters. How SVs cross the dense mesh of the cortical actin network to reach the plasma membrane remains unclear. Here we reveal that, in bovine chromaffin cells, SVs embedded in the cortical actin network undergo a highly synchronized transition towards the plasma membrane and Munc18-1-dependent docking in response to secretagogues. This movement coincides with a translocation of the cortical actin network in the same direction. Both effects are abolished by the knockdown or the pharmacological inhibition of myosin II, suggesting changes in actomyosin-generated forces across the cell cortex. Indeed, we report a reduction in cortical actin network tension elicited on secretagogue stimulation that is sensitive to myosin II inhibition. We reveal that the cortical actin network acts as a 'casting net' that undergoes activity-dependent relaxation, thereby driving tethered SVs towards the plasma membrane where they undergo Munc18-1-dependent docking. |
| المشرفين على المادة: | 0 (Actins) 0 (Heterocyclic Compounds, 4 or More Rings) 0 (Munc18 Proteins) 20WC4J7CQ6 (blebbistatin) EC 3.6.1.- (Myosin Type II) |
| تواريخ الأحداث: | Date Created: 20150225 Date Completed: 20160315 Latest Revision: 20161125 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1038/ncomms7297 |
| PMID: | 25708831 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2041-1723 |
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| DOI: | 10.1038/ncomms7297 |