دورية أكاديمية
أعرض في EDS

Evaluation of ViroCyt® Virus Counter for rapid filovirus quantitation.

التفاصيل البيبلوغرافية
العنوان: Evaluation of ViroCyt® Virus Counter for rapid filovirus quantitation.
المؤلفون: Rossi CA; Diagnostic Systems Division, US Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA. cynthia.a.rossi.civ@mail.mil., Kearney BJ; Diagnostic Systems Division, US Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA. brian.j.kearney.civ@mail.mil., Olschner SP; Diagnostic Systems Division, US Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA. scott.p.olschner.civ@mail.mil., Williams PL; Diagnostic Systems Division, US Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA. priscilla.l.williams4.ctr@mail.mil., Robinson CG; Pathology Division, US Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA. robinsonc@janelia.hhmi.org., Heinrich ML; Diagnostic Systems Division, US Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA. mheinrich7295@gmail.com., Zovanyi AM; Diagnostic Systems Division, US Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA. azovanyi@gmail.com., Ingram MF; Diagnostic Systems Division, US Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA. michael.f.ingram.mil@mail.mil., Norwood DA; Diagnostic Systems Division, US Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA. david.a.norwood.civ@mail.mil., Schoepp RJ; Diagnostic Systems Division, US Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA. randal.j.schoepp.civ@mail.mil.
المصدر: Viruses [Viruses] 2015 Feb 20; Vol. 7 (3), pp. 857-72. Date of Electronic Publication: 2015 Feb 20.
نوع المنشور: Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101509722 Publication Model: Electronic Cited Medium: Internet ISSN: 1999-4915 (Electronic) Linking ISSN: 19994915 NLM ISO Abbreviation: Viruses Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI
مواضيع طبية MeSH: Ebolavirus/*isolation & purification , Viral Load/*methods, Animals ; Humans ; Staining and Labeling/methods ; Time Factors
مستخلص: Development and evaluation of medical countermeasures for diagnostics, vaccines, and therapeutics requires production of standardized, reproducible, and well characterized virus preparations. For filoviruses this includes plaque assay for quantitation of infectious virus, transmission electron microscopy (TEM) for morphology and quantitation of virus particles, and real-time reverse transcription PCR for quantitation of viral RNA (qRT-PCR). The ViroCyt® Virus Counter (VC) 2100 (ViroCyt, Boulder, CO, USA) is a flow-based instrument capable of quantifying virus particles in solution. Using a proprietary combination of fluorescent dyes that stain both nucleic acid and protein in a single 30 min step, rapid, reproducible, and cost-effective quantification of filovirus particles was demonstrated. Using a seed stock of Ebola virus variant Kikwit, the linear range of the instrument was determined to be 2.8E+06 to 1.0E+09 virus particles per mL with coefficient of variation ranging from 9.4% to 31.5% for samples tested in triplicate. VC particle counts for various filovirus stocks were within one log of TEM particle counts. A linear relationship was established between the plaque assay, qRT-PCR, and the VC. VC results significantly correlated with both plaque assay and qRT-PCR. These results demonstrated that the VC is an easy, fast, and consistent method to quantify filoviruses in stock preparations.
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تواريخ الأحداث: Date Created: 20150225 Date Completed: 20151109 Latest Revision: 20191210
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC4379551
DOI: 10.3390/v7030857
PMID: 25710889
قاعدة البيانات: MEDLINE
الوصف
تدمد:1999-4915
DOI:10.3390/v7030857