دورية أكاديمية
Ultrasensitive detection of drug resistant cancer cells in biological matrixes using an amperometric nanobiosensor.
| العنوان: | Ultrasensitive detection of drug resistant cancer cells in biological matrixes using an amperometric nanobiosensor. |
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| المؤلفون: | Chandra P; Department of Chemistry and Institute of Biophysio Sensor Technology (IBST), Pusan National University, Busan 609-735, South Korea; Amity Institute of Biotechnology, Amity University Uttar Pradesh, Noida 201303, U.P., India., Noh HB; Department of Chemistry and Institute of Biophysio Sensor Technology (IBST), Pusan National University, Busan 609-735, South Korea., Pallela R; Department of Chemistry and Institute of Biophysio Sensor Technology (IBST), Pusan National University, Busan 609-735, South Korea., Shim YB; Department of Chemistry and Institute of Biophysio Sensor Technology (IBST), Pusan National University, Busan 609-735, South Korea. Electronic address: ybshim@pusan.ac.kr. |
| المصدر: | Biosensors & bioelectronics [Biosens Bioelectron] 2015 Aug 15; Vol. 70, pp. 418-25. Date of Electronic Publication: 2015 Mar 27. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Advanced Technology Country of Publication: England NLM ID: 9001289 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4235 (Electronic) Linking ISSN: 09565663 NLM ISO Abbreviation: Biosens Bioelectron Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oxford : Elsevier Advanced Technology Original Publication: [Barking, Essex, England] : Elsevier Applied Science, 1989- |
| مواضيع طبية MeSH: | ATP Binding Cassette Transporter, Subfamily B, Member 1/*analysis , Biomarkers, Tumor/*analysis , Biosensing Techniques/*instrumentation , Metal Nanoparticles/*chemistry , Neoplasms, Experimental/*chemistry , Neoplasms, Experimental/*diagnosis, Cell Line, Tumor ; Drug Resistance, Neoplasm ; Equipment Design ; Equipment Failure Analysis ; Gold/chemistry ; Humans ; Immunoassay/instrumentation ; Microchemistry/instrumentation ; Nanomedicine/instrumentation ; Reproducibility of Results ; Sensitivity and Specificity |
| مستخلص: | Multidrug resistance (MDR) is a key issue in the failure of cancer chemotherapy and its detection will be helpful to develop suitable therapeutic strategies for cancer patients and overcome the death rates. In this direction, we designed a new amperometric sensor (a medical device prototype) to detect drug resistant cancer cells by sensing "Permeability glycoprotein (P-gp)". The sensor probe is fabricated by immobilizing monoclonal P-gp antibody on the gold nanoparticles (AuNPs) conducting polymer composite. The detection relies on a sandwich-type approach using a bioconjugate, where the aminophenyl boronic acid (APBA) served as a recognition molecule which binds with the cell surface glycans and hydrazine (Hyd) served as an electrocatalyst for the reduction of H2O2 which are attached on multi-wall carbon nanotube (MWCNT) (APBA-MWCNT-Hyd). A linear range for the cancer cell detection is obtained between 50 and 100,000 cells/mL with the detection limit of 23±2 cells/mL. The proposed immunosensor is successfully applied to detect MDR cancer cells (MDRCC) in serum and mixed cell samples. Interferences by drug sensitive (SKBr-3 and HeLa), noncancerous cells (HEK-293 and OSE), and other chemical molecules present in the real sample matrix are examined. The sensitivity of the proposed immunosensor is excellent compared with the conventional reporter antibody based assay. (Copyright © 2015 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Drug resistant cancer cells; Electrochemistry; In vitro test; Nanobiosensor; Nanomaterials |
| المشرفين على المادة: | 0 (ATP Binding Cassette Transporter, Subfamily B, Member 1) 0 (Biomarkers, Tumor) 7440-57-5 (Gold) |
| تواريخ الأحداث: | Date Created: 20150408 Date Completed: 20160222 Latest Revision: 20181202 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.bios.2015.03.069 |
| PMID: | 25845334 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-4235 |
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| DOI: | 10.1016/j.bios.2015.03.069 |