دورية أكاديمية
أعرض في EDS

Antibody microarrays utilizing site-specific antibody-oligonucleotide conjugates.

التفاصيل البيبلوغرافية
العنوان: Antibody microarrays utilizing site-specific antibody-oligonucleotide conjugates.
المؤلفون: Wold ED, McBride R, Axup JY, Kazane SA; ‡California Institute for Biomedical Research (Calibr), 11119 North Torrey Pines Road, La Jolla, California 92037, United States., Smider VV
المصدر: Bioconjugate chemistry [Bioconjug Chem] 2015 May 20; Vol. 26 (5), pp. 807-11. Date of Electronic Publication: 2015 Apr 30.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: American Chemical Society Country of Publication: United States NLM ID: 9010319 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-4812 (Electronic) Linking ISSN: 10431802 NLM ISO Abbreviation: Bioconjug Chem Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, DC : American Chemical Society, c1990-
مواضيع طبية MeSH: Immunoglobulin Fab Fragments/*metabolism , Oligonucleotides/*metabolism , Protein Array Analysis/*methods, Binding Sites ; Cell Line, Tumor ; Cells, Immobilized/metabolism ; DNA/chemistry ; DNA/metabolism ; Humans ; Immunoglobulin Fab Fragments/chemistry ; Immunoglobulin Fab Fragments/immunology ; Models, Molecular ; Nucleic Acid Conformation ; Oligonucleotides/chemistry ; Phenylalanine/chemistry ; Receptor, ErbB-2/immunology ; Substrate Specificity
مستخلص: Protein arrays are typically made by random absorption of proteins to the array surface, potentially limiting the amount of properly oriented and functional molecules. We report the development of a DNA encoded antibody microarray utilizing site-specific antibody-oligonucleotide conjugates that can be used for cell immobilization as well as the detection of genes and proteins. This technology allows for the facile generation of antibody microarrays while circumventing many of the drawbacks of conventionally produced antibody arrays. We demonstrate that this method can be used to capture and detect SK-BR-3 cells (Her2+ breast cancer cells) at concentrations as low as 10(2) cells/mL (which is equivalent to 10 cells per 100 μL array) without the use of microfluidics, which is 100- to 10(5)-fold more sensitive than comparable techniques. Additionally, the method was shown to be able to detect cells in a complex mixture, effectively immobilizing and specifically detecting Her2+ cells at a concentration of 10(2) SK-BR-3 cells/mL in 4 × 10(6) white blood cells/mL. Patients with a variety of cancers can have circulating tumor cell counts of between 1 and 10(3) cells/mL in whole blood, well within the range of this technology.
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معلومات مُعتمدة: RC1 EB010745 United States EB NIBIB NIH HHS
المشرفين على المادة: 0 (Immunoglobulin Fab Fragments)
0 (Oligonucleotides)
47E5O17Y3R (Phenylalanine)
9007-49-2 (DNA)
EC 2.7.10.1 (ERBB2 protein, human)
EC 2.7.10.1 (Receptor, ErbB-2)
تواريخ الأحداث: Date Created: 20150418 Date Completed: 20160211 Latest Revision: 20181113
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC4787600
DOI: 10.1021/acs.bioconjchem.5b00111
PMID: 25884500
قاعدة البيانات: MEDLINE
الوصف
تدمد:1520-4812
DOI:10.1021/acs.bioconjchem.5b00111