دورية أكاديمية
In vitro RNA uptake studies in plant mitochondria.
| العنوان: | In vitro RNA uptake studies in plant mitochondria. |
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| المؤلفون: | Kubiszewski-Jakubiak S; Australian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009, Australia., Megel C, Ubrig E, Salinas T, Duchêne AM, Maréchal-Drouard L |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2015; Vol. 1305, pp. 45-60. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Arabidopsis/*metabolism , Mitochondria/*metabolism , RNA, Plant/*metabolism , RNA, Transfer/*metabolism , Solanum tuberosum/*metabolism, Arabidopsis/genetics ; Electrophoresis, Polyacrylamide Gel/methods ; Mitochondria/genetics ; RNA Transport ; RNA, Plant/genetics ; RNA, Transfer/genetics ; Reverse Transcriptase Polymerase Chain Reaction/methods ; Solanum tuberosum/genetics ; Transcription, Genetic |
| مستخلص: | During evolution, most of the ancestral genes from the endosymbiotic α-proteobacteria at the origin of mitochondria have been either lost or transferred to the nuclear genome. To allow the comeback of proteins and RNAs [in particular transfer RNA (tRNAs)] into the organelle, macromolecule import systems were universally established. While protein import processes have been studied into details, much less is known about tRNA mitochondrial import. In plants, part of the knowledge on the tRNA import process into mitochondria has been acquired thanks to in vitro import assays. Furthermore, the development of in vitro RNA import strategies allowed the study of plant mitochondrial gene expression. The purpose of this chapter is to provide detailed protocols to perform in vitro RNA uptake into potato (Solanum tuberosum) or Arabidopsis (Arabidopsis thaliana) mitochondria as well as approaches to analyze them. |
| المشرفين على المادة: | 0 (RNA, Plant) 9014-25-9 (RNA, Transfer) |
| تواريخ الأحداث: | Date Created: 20150426 Date Completed: 20160115 Latest Revision: 20150427 |
| رمز التحديث: | 20230127 |
| DOI: | 10.1007/978-1-4939-2639-8_4 |
| PMID: | 25910726 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-6029 |
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| DOI: | 10.1007/978-1-4939-2639-8_4 |