دورية أكاديمية
أعرض في EDS

Capillary electrophoresis applied to DNA: determining and harnessing sequence and structure to advance bioanalyses (2009-2014).

التفاصيل البيبلوغرافية
العنوان: Capillary electrophoresis applied to DNA: determining and harnessing sequence and structure to advance bioanalyses (2009-2014).
المؤلفون: Durney BC; C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV, 26506, USA., Crihfield CL, Holland LA
المصدر: Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2015 Sep; Vol. 407 (23), pp. 6923-38. Date of Electronic Publication: 2015 May 03.
نوع المنشور: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Review
اللغة: English
بيانات الدورية: Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 101134327 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-2650 (Electronic) Linking ISSN: 16182642 NLM ISO Abbreviation: Anal Bioanal Chem Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Heidelberg : Springer-Verlag, 2002-
مواضيع طبية MeSH: DNA/*analysis , DNA/*genetics , Electrophoresis, Capillary/*instrumentation , Electrophoresis, Capillary/*methods , Sequence Analysis, DNA/*instrumentation , Sequence Analysis, DNA/*methods, Aptamers, Nucleotide/analysis ; Aptamers, Nucleotide/chemical synthesis ; Aptamers, Nucleotide/genetics ; DNA/chemistry ; Drug Design
مستخلص: This review of capillary electrophoresis methods for DNA analyses covers critical advances from 2009 to 2014, referencing 184 citations. Separation mechanisms based on free-zone capillary electrophoresis, Ogston sieving, and reptation are described. Two prevalent gel matrices for gel-facilitated sieving, which are linear polyacrylamide and polydimethylacrylamide, are compared in terms of performance, cost, viscosity, and passivation of electroosmotic flow. The role of capillary electrophoresis in the discovery, design, and characterization of DNA aptamers for molecular recognition is discussed. Expanding and emerging techniques in the field are also highlighted.
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المشرفين على المادة: 0 (Aptamers, Nucleotide)
9007-49-2 (DNA)
تواريخ الأحداث: Date Created: 20150504 Date Completed: 20160523 Latest Revision: 20190109
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC4551542
DOI: 10.1007/s00216-015-8703-5
PMID: 25935677
قاعدة البيانات: MEDLINE
الوصف
تدمد:1618-2650
DOI:10.1007/s00216-015-8703-5