دورية أكاديمية
Detection and quantification of microRNA in cerebral microdialysate.
| العنوان: | Detection and quantification of microRNA in cerebral microdialysate. |
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| المؤلفون: | Bache S; Department of Neuroanaesthesiology, The Neuroscience Centre, Copenhagen University Hospital, Copenhagen, Denmark. bache@dadlnet.dk.; Centre for Genomic Medicine, The Diagnostic Centre, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark. bache@dadlnet.dk., Rasmussen R; Department of Neurosurgery, The Neuroscience Centre, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark. rune333@gmail.com., Rossing M; Centre for Genomic Medicine, The Diagnostic Centre, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark. rossing.maria@gmail.com., Hammer NR; Department of Neuroanaesthesiology, The Neuroscience Centre, Copenhagen University Hospital, Copenhagen, Denmark. nahammer@hotmail.com., Juhler M; Department of Neurosurgery, The Neuroscience Centre, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark. marianne.juhler@gmail.com., Friis-Hansen L; Centre for Genomic Medicine, The Diagnostic Centre, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark. ejf@regionsjaelland.dk.; Present address: Department of Clinical Biochemistry, Naestved Sygehus, Naestved, Denmark. ejf@regionsjaelland.dk., Nielsen FC; Centre for Genomic Medicine, The Diagnostic Centre, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark. finn.cilius.nielsen@regionh.dk., Møller K; Department of Neuroanaesthesiology, The Neuroscience Centre, Copenhagen University Hospital, Copenhagen, Denmark. kirsten.moller@dadlnet.dk. |
| المصدر: | Journal of translational medicine [J Transl Med] 2015 May 07; Vol. 13, pp. 149. Date of Electronic Publication: 2015 May 07. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: BioMed Central Country of Publication: England NLM ID: 101190741 Publication Model: Electronic Cited Medium: Internet ISSN: 1479-5876 (Electronic) Linking ISSN: 14795876 NLM ISO Abbreviation: J Transl Med Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [London] : BioMed Central, 2003- |
| مواضيع طبية MeSH: | Cerebrospinal Fluid/*chemistry , MicroRNAs/*cerebrospinal fluid , Microdialysis/*methods , Subarachnoid Hemorrhage/*cerebrospinal fluid, Brain/metabolism ; Brain Injuries/cerebrospinal fluid ; Humans ; Perfusion ; RNA Processing, Post-Transcriptional ; Reproducibility of Results ; Temperature ; Treatment Outcome |
| مستخلص: | Background: Secondary brain injury accounts for a major part of the morbidity and mortality in patients with spontaneous aneurysmal subarachnoid hemorrhage (SAH), but the pathogenesis and pathophysiology remain controversial. MicroRNAs (miRNAs) are important posttranscriptional regulators of complementary mRNA targets and have been implicated in the pathophysiology of other types of acute brain injury. Cerebral microdialysis is a promising tool to investigate these mechanisms. We hypothesized that miRNAs would be present in human cerebral microdialysate. Methods: RNA was extracted and miRNA profiles were established using high throughput real-time quantification PCR on the following material: 1) Microdialysate sampled in vitro from A) a solution of total RNA extracted from human brain, B) cerebrospinal fluid (CSF) from a neurologically healthy patient, and C) a patient with SAH; and 2) cerebral microdialysate and CSF sampled in vivo from two patients with SAH. MiRNAs were categorized according to their relative recovery (RR) and a pathway analysis was performed for miRNAs exhibiting a high RR in vivo. Results: Seventy-one of the 160 miRNAs detected in CSF were also found in in vivo microdialysate from SAH patients. Furthermore specific miRNAs consistently exhibited either a high or low RR in both in vitro and in vivo microdialysate. Analysis of repeatability showed lower analytical variation in microdialysate than in CSF. Conclusions: MiRNAs are detectable in cerebral microdialysate; a large group of miRNAs consistently showed a high RR in cerebral microdialysate. Measurement of cerebral interstitial miRNA concentrations may aid in the investigation of secondary brain injury in neurocritical conditions. |
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| المشرفين على المادة: | 0 (MicroRNAs) |
| تواريخ الأحداث: | Date Created: 20150508 Date Completed: 20160419 Latest Revision: 20181113 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC4438475 |
| DOI: | 10.1186/s12967-015-0505-1 |
| PMID: | 25947950 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1479-5876 |
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| DOI: | 10.1186/s12967-015-0505-1 |