دورية أكاديمية
أعرض في EDS

Identification and characterization of a nuclear localization signal of TRIM28 that overlaps with the HP1 box.

التفاصيل البيبلوغرافية
العنوان: Identification and characterization of a nuclear localization signal of TRIM28 that overlaps with the HP1 box.
المؤلفون: Moriyama T; Laboratory of Nuclear Transport Dynamics, National Institutes of Biomedical Innovation, Health and Nutrition (NIBIOHN), Osaka 567-0085, Japan., Sangel P; Laboratory of Nuclear Transport Dynamics, National Institutes of Biomedical Innovation, Health and Nutrition (NIBIOHN), Osaka 567-0085, Japan., Yamaguchi H; School of Medicine, Osaka University, Osaka 565-0871, Japan., Obuse C; Graduate School of Life Science, Hokkaido University, Sapporo 001-0021, Japan., Miyamoto Y; Laboratory of Nuclear Transport Dynamics, National Institutes of Biomedical Innovation, Health and Nutrition (NIBIOHN), Osaka 567-0085, Japan., Oka M; Laboratory of Nuclear Transport Dynamics, National Institutes of Biomedical Innovation, Health and Nutrition (NIBIOHN), Osaka 567-0085, Japan; Laboratory of Biomedical Innovation, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka 565-0871, Japan. Electronic address: moka@nibiohn.go.jp., Yoneda Y; National Institutes of Biomedical Innovation, Health and Nutrition (NIBIOHN), Osaka 567-0085, Japan; Laboratory of Biomedical Innovation, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka 565-0871, Japan. Electronic address: y-yoneda@nibiohn.go.jp.
المصدر: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2015 Jul 03; Vol. 462 (3), pp. 201-7. Date of Electronic Publication: 2015 May 07.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2104 (Electronic) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE
أسماء مطبوعة: Publication: <2002- >: San Diego, CA : Elsevier
Original Publication: New York, Academic Press.
مواضيع طبية MeSH: Chromosomal Proteins, Non-Histone/*chemistry , Chromosomal Proteins, Non-Histone/*metabolism , Nuclear Localization Signals/*chemistry , Nuclear Localization Signals/*metabolism , Repressor Proteins/*chemistry , Repressor Proteins/*metabolism, Active Transport, Cell Nucleus ; Amino Acid Sequence ; Binding Sites ; Chromobox Protein Homolog 5 ; Chromosomal Proteins, Non-Histone/genetics ; HeLa Cells ; Humans ; Models, Biological ; Molecular Sequence Data ; Nuclear Localization Signals/genetics ; Protein Binding ; Protein Interaction Domains and Motifs ; Recombinant Fusion Proteins/chemistry ; Recombinant Fusion Proteins/genetics ; Recombinant Fusion Proteins/metabolism ; Repressor Proteins/genetics ; Tripartite Motif-Containing Protein 28 ; alpha Karyopherins/chemistry ; alpha Karyopherins/classification ; alpha Karyopherins/metabolism ; beta Karyopherins/chemistry ; beta Karyopherins/classification ; beta Karyopherins/metabolism
مستخلص: Tripartite motif-containing 28 (TRIM28) is a transcription regulator, which forms a repressor complex containing heterochromatin protein 1 (HP1). Here, we report identification of a nuclear localization signal (NLS) within the 462-494 amino acid region of TRIM28 that overlaps with its HP1 binding site, HP1 box. GST-pulldown experiments revealed the interaction of the arginine-rich TRIM28 NLS with various importin α subtypes (α1, α2 and α4). In vitro transport assay demonstrated that nuclear localization of GFP-TRIM28 NLS is mediated by importin αs, in conjunction with importin β1 and Ran. Further, we demonstrated that HP1 and importin αs compete for binding to TRIM28. Together, our findings suggest that importin α has an essential role in the nuclear delivery and preferential HP1 interaction of TRIM28.
(Copyright © 2015 Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: HP1; Importin α; KAP1; NLS; TIF1β; TRIM28
المشرفين على المادة: 0 (Chromosomal Proteins, Non-Histone)
0 (Nuclear Localization Signals)
0 (Recombinant Fusion Proteins)
0 (Repressor Proteins)
0 (alpha Karyopherins)
0 (beta Karyopherins)
107283-02-3 (Chromobox Protein Homolog 5)
EC 2.3.2.27 (TRIM28 protein, human)
EC 2.3.2.27 (Tripartite Motif-Containing Protein 28)
تواريخ الأحداث: Date Created: 20150512 Date Completed: 20150817 Latest Revision: 20211203
رمز التحديث: 20221213
DOI: 10.1016/j.bbrc.2015.04.108
PMID: 25960296
قاعدة البيانات: MEDLINE
الوصف
تدمد:1090-2104
DOI:10.1016/j.bbrc.2015.04.108