دورية أكاديمية
Lupalbigenin from Derris scandens Sensitizes Detachment-induced Cell Death in Human Lung Cancer Cells.
| العنوان: | Lupalbigenin from Derris scandens Sensitizes Detachment-induced Cell Death in Human Lung Cancer Cells. |
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| المؤلفون: | Ausawasamrit A; Department of Pharmacology and Physiology, Chulalongkorn University, Bangkok, Thailand Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand., Itthiwarapornkul N; Department of Pharmacology and Physiology, Chulalongkorn University, Bangkok, Thailand Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand., Chaotham C; Department of Biochemistry and Microbiology, Chulalongkorn University, Bangkok, Thailand Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand., Sukrong S; Department of Pharmacognosy and Pharmaceutical Botany, Chulalongkorn University, Bangkok, Thailand Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand pithi_chan@yahoo.com Suchada.su@chula.ac.th., Chanvorachote P; Department of Pharmacology and Physiology, Chulalongkorn University, Bangkok, Thailand Cell-Based Drug and Health Product Development Research Unit, Chulalongkorn University, Bangkok, Thailand Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand pithi_chan@yahoo.com Suchada.su@chula.ac.th. |
| المصدر: | Anticancer research [Anticancer Res] 2015 May; Vol. 35 (5), pp. 2827-34. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: International Institute of Anticancer Research Country of Publication: Greece NLM ID: 8102988 Publication Model: Print Cited Medium: Internet ISSN: 1791-7530 (Electronic) Linking ISSN: 02507005 NLM ISO Abbreviation: Anticancer Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Attiki, Greece : International Institute of Anticancer Research Original Publication: Athens, Greece : Potamitis Press |
| مواضيع طبية MeSH: | Anoikis/*drug effects , Apoptosis Regulatory Proteins/*biosynthesis , Isoflavones/*administration & dosage , Lung Neoplasms/*drug therapy , Plant Extracts/*administration & dosage, Apoptosis/drug effects ; Apoptosis Regulatory Proteins/genetics ; Cell Line, Tumor ; Derris/chemistry ; Gene Expression Regulation, Neoplastic/drug effects ; Humans ; Lung Neoplasms/genetics ; Lung Neoplasms/pathology ; Mitogen-Activated Protein Kinases/biosynthesis ; Neoplasm Metastasis ; Plant Extracts/chemistry ; Proto-Oncogene Proteins c-bcl-2/biosynthesis ; Signal Transduction/drug effects |
| مستخلص: | Background/aim: The ability of cancer cells to resist to anoikis has been shown to augment cancer cell metastasis in many cancers. In search for potential substances for anti-metastatic approaches, this study aimed to investigate anoikis-sensitizing activity of lupalbigenin, extracted from Derris scandens. Materials and Methods: Human lung cancer cells were treated with non-cytotoxic concentrations of lupalbigenin in a detachment condition. Anoikis was evaluated at various time points using MTT viability assays. The effect of lupalbigenin on anchorage-independent growth was performed by soft-agar assay. The survival signaling proteins, as well as regulatory proteins of apoptosis and metastasis, were examined by western blot analysis. Results: Lupalbigenin treatment significantly down-regulated survival proteins, including protein kinase B (pAKT/AKT) and extracellular signal-regulated kinase (pERK/ERK), as well as anti-apoptotic protein B-cell lymphoma 2 (BCL-2), resulting in the enhancement of the cellular response to anoikis and the decrease of growth and survival in an anchorage-independent condition. Conclusion: Lupalbigenin sensitizes detachment-induced cell death in human lung cancer cell through down-regulation of pro-survival proteins. (Copyright© 2015 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.) |
| فهرسة مساهمة: | Keywords: Derris scandens; Lung cancer; anoikis; lupalbigenin; pro-survival pathway |
| المشرفين على المادة: | 0 (Apoptosis Regulatory Proteins) 0 (Isoflavones) 0 (Plant Extracts) 0 (Proto-Oncogene Proteins c-bcl-2) 0RZW2IS86C (lupalbigenin) EC 2.7.11.24 (Mitogen-Activated Protein Kinases) |
| تواريخ الأحداث: | Date Created: 20150513 Date Completed: 20150806 Latest Revision: 20181212 |
| رمز التحديث: | 20240829 |
| PMID: | 25964563 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1791-7530 |
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