دورية أكاديمية
[Role of the glutathione system in regulation of redox status in the rat cerebral cortex under hypoxia].
| العنوان: | [Role of the glutathione system in regulation of redox status in the rat cerebral cortex under hypoxia]. |
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| المؤلفون: | Kirova YI |
| المصدر: | Patologicheskaia fiziologiia i eksperimental'naia terapiia [Patol Fiziol Eksp Ter] 2014 Oct-Dec (4), pp. 40-7. |
| نوع المنشور: | Journal Article |
| اللغة: | Russian |
| بيانات الدورية: | Publisher: Izdatelstvo Meditsina Country of Publication: Russia (Federation) NLM ID: 0376421 Publication Model: Print Cited Medium: Print ISSN: 0031-2991 (Print) Linking ISSN: 00312991 NLM ISO Abbreviation: Patol Fiziol Eksp Ter Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Moskva : Izdatelstvo Meditsina |
| مواضيع طبية MeSH: | Oxidative Stress*, Antioxidants/*metabolism , Cerebral Cortex/*metabolism , Glutathione/*metabolism , Hypoxia/*metabolism, Adaptation, Physiological ; Animals ; Disease Models, Animal ; Free Radicals/metabolism ; Glutathione Disulfide/metabolism ; Lipid Peroxidation ; Lipid Peroxides/metabolism ; Male ; Rats |
| مستخلص: | The study compared effects of different hypobaric hypoxia regimens (14% O2, 10.5% O2, and 8% O2; 1 hour; 15 days) on parameters of the glutathione system, intensity of free radical oxidation, and intensity of lipid peroxidation in cerebral cortex (CC) of rats with different, genetically predetermined resistance to acute hypoxia. In normoxia, baseline concentrations of oxidized glutathione (0.155 ± 0.011 nmol/mg protein) and hydroperoxide metabolites (50.4 ± 2.62 cumene hydroperoxide equivalents/g tissue) were 20% lower in CC of low-resistance (LR) rats than in high-resistance (HR) rats (0.191 ± 0.013 nmol/mg protein; 63.0 ± 3.46 cumene hydroperoxide equivalents/g tissue, respectively). Baseline activities of the glutathione cycle enzymes, glutathione peroxidase (31.2 ± 1.59 nmol/min/mg protein) and glutathione reductase (28.5 ± 1.49 nmol/min/mg protein), were 30% lower in LR rat CC than in HR rat CC (47.0 ± 2.41; 433 ± 2.26 nmol/min/mg protein, respectively). These data suggested more efficient antioxidant defense of CC tissue in LR rats than in HR rats. The phenotypic difference in CC tissue redox properties between two rat phenotypes remained in hypoxia. The efficiency of glutathione system in regulation of CC redox homeostasis was shown to depend on both severity and duration of hypoxic exposures and on individual tolerability of hypoxia, i.e., this efficiency was genetically predetermined. The glutathione system maintains its regulatory properties in a broader range of lowered pO2 values and during longer hypoxic exposures. in LR rat CC than in HR rat CC. For this reason, activation of free radical processes and development of oxidative stress induced by single or repeated hypoxic exposures are prevented or alleviated in LR rat CC but not in HR rat CC. The obtained data strongly justify the need for selection of hypoxic exposure regimens for therapeutic hypoxic preconditioning. Apparently the hypoxic exposures not associated with signs of oxidative stress should be considered optimum. |
| المشرفين على المادة: | 0 (Antioxidants) 0 (Free Radicals) 0 (Lipid Peroxides) GAN16C9B8O (Glutathione) ULW86O013H (Glutathione Disulfide) |
| تواريخ الأحداث: | Date Created: 20150519 Date Completed: 20150602 Latest Revision: 20161125 |
| رمز التحديث: | 20221213 |
| PMID: | 25980225 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0031-2991 |
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