دورية أكاديمية
Novel Approaches and Technologies in Molecular HLA Typing.
| العنوان: | Novel Approaches and Technologies in Molecular HLA Typing. |
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| المؤلفون: | Dunn PP; Leicester General Hospital, University Hospitals Leicester NHS Trust, Gwendolen Road, Leicester, LE5 4PW, UK, paul.dunn@uhl-tr.nhs.uk. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2015; Vol. 1310, pp. 213-30. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Polymorphism, Genetic*, HLA Antigens/*genetics , High-Throughput Nucleotide Sequencing/*methods, Genotype ; Genotyping Techniques/methods ; Histocompatibility Testing/methods ; Humans ; Oligonucleotide Array Sequence Analysis/methods ; Real-Time Polymerase Chain Reaction/methods ; Sequence Analysis, RNA/methods |
| مستخلص: | The invention of the Polymerase Chain Reaction (PCR) has revolutionized molecular biology enabling gene isolation and characterization in hours rather than days. Scientists working in transplant diagnostics have proven to be pioneers in adapting this molecular technique to the clinical needs of histocompatibility testing. This chapter describes a number of novel genotyping technologies which have been used to address the challenges posed by genetic diversity seen in the extensive polymorphism in HLA genes. These novel approaches include single-stranded and duplex conformational analyses, real-time PCR, microarray hybridization, RNA-based sequencing, and the present day Next Generation Sequencing. The chapter concludes with a brief look at a possible next, Next Generation Sequencing system. |
| المشرفين على المادة: | 0 (HLA Antigens) |
| تواريخ الأحداث: | Date Created: 20150531 Date Completed: 20160229 Latest Revision: 20150530 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1007/978-1-4939-2690-9_18 |
| PMID: | 26024638 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-6029 |
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| DOI: | 10.1007/978-1-4939-2690-9_18 |