دورية أكاديمية
أعرض في EDS

Identification of interleukin-26 in the dromedary camel (Camelus dromedarius): Evidence of alternative splicing and isolation of novel splice variants.

التفاصيل البيبلوغرافية
العنوان: Identification of interleukin-26 in the dromedary camel (Camelus dromedarius): Evidence of alternative splicing and isolation of novel splice variants.
المؤلفون: Premraj A; Camel Biotechnology Center, Management of Scientific Centers and the Presidential Camels, Department of the President's Affairs, P O Box 17292, Al Ain, United Arab Emirates., Nautiyal B; Camel Biotechnology Center, Management of Scientific Centers and the Presidential Camels, Department of the President's Affairs, P O Box 17292, Al Ain, United Arab Emirates., Aleyas AG; Camel Biotechnology Center, Management of Scientific Centers and the Presidential Camels, Department of the President's Affairs, P O Box 17292, Al Ain, United Arab Emirates., Rasool TJ; Camel Biotechnology Center, Management of Scientific Centers and the Presidential Camels, Department of the President's Affairs, P O Box 17292, Al Ain, United Arab Emirates. Electronic address: tjrasool@gmail.com.
المصدر: Molecular immunology [Mol Immunol] 2015 Oct; Vol. 67 (2 Pt B), pp. 357-68. Date of Electronic Publication: 2015 Jul 17.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Pergamon Press Country of Publication: England NLM ID: 7905289 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-9142 (Electronic) Linking ISSN: 01615890 NLM ISO Abbreviation: Mol Immunol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Oxford, Elmsford, N. Y., Pergamon Press.
مواضيع طبية MeSH: Alternative Splicing/*genetics , Camelus/*genetics , Interleukins/*genetics, Alternative Splicing/drug effects ; Amino Acid Sequence ; Animals ; Cloning, Molecular ; DNA, Complementary/genetics ; Exons/genetics ; Female ; Fibroblasts/drug effects ; Fibroblasts/metabolism ; Fibroblasts/virology ; Gene Expression Profiling ; Interleukins/metabolism ; Introns/genetics ; Leukocytes, Mononuclear/drug effects ; Leukocytes, Mononuclear/metabolism ; Mitogens/pharmacology ; Molecular Sequence Data ; Orthopoxvirus ; Ovary/pathology ; Phylogeny ; Poxviridae Infections/genetics ; Poxviridae Infections/pathology ; Poxviridae Infections/virology ; Protein Isoforms/genetics ; Protein Isoforms/metabolism ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Sequence Alignment ; Structural Homology, Protein
مستخلص: Interleukin-26 (IL-26) is a member of the IL-10 family of cytokines. Though conserved across vertebrates, the IL-26 gene is functionally inactivated in a few mammals like rat, mouse and horse. We report here the identification, isolation and cloning of the cDNA of IL-26 from the dromedary camel. The camel cDNA contains a 516 bp open reading frame encoding a 171 amino acid precursor protein, including a 21 amino acid signal peptide. Sequence analysis revealed high similarity with other mammalian IL-26 homologs and the conservation of IL-10 cytokine family domain structure including key amino acid residues. We also report the identification and cloning of four novel transcript variants produced by alternative splicing at the Exon 3-Exon 4 regions of the gene. Three of the alternative splice variants had premature termination codons and are predicted to code for truncated proteins. The transcript variant 4 (Tv4) having an insertion of an extra 120 bp nucleotides in the ORF was predicted to encode a full length protein product with 40 extra amino acid residues. The mRNA transcripts of all the variants were identified in lymph node, where as fewer variants were observed in other tissues like blood, liver and kidney. The expression of Tv2 and Tv3 were found to be up regulated in mitogen induced camel peripheral blood mononuclear cells. IL-26-Tv2 expression was also induced in camel fibroblast cells infected with Camel pox virus in-vitro. The identification of the transcript variants of IL-26 from the dromedary camel is the first report of alternative splicing for IL-26 in a species in which the gene has not been inactivated.
(Copyright © 2015 Elsevier Ltd. All rights reserved.)
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فهرسة مساهمة: Keywords: Alternative splicing; Camelids; Cytokine; IL-10; IL-26; Virus
المشرفين على المادة: 0 (DNA, Complementary)
0 (Interleukins)
0 (Mitogens)
0 (Protein Isoforms)
0 (RNA, Messenger)
تواريخ الأحداث: Date Created: 20150721 Date Completed: 20151215 Latest Revision: 20200407
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC7112506
DOI: 10.1016/j.molimm.2015.06.022
PMID: 26190308
قاعدة البيانات: MEDLINE
الوصف
تدمد:1872-9142
DOI:10.1016/j.molimm.2015.06.022