دورية أكاديمية
Predominance of Cryptosporidium parvum genotype among diarrheic children from Egypt as an indicator for zoonotic transmission.
| العنوان: | Predominance of Cryptosporidium parvum genotype among diarrheic children from Egypt as an indicator for zoonotic transmission. |
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| المؤلفون: | Eraky MA, El-Hamshary AM, Hamadto HH, Abdallah KF, Abdel-Hafed WM, Abdel-Had S |
| المصدر: | Acta parasitologica [Acta Parasitol] 2014 Mar; Vol. 60 (1), pp. 26-34. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer International Publishing Country of Publication: Switzerland NLM ID: 9301947 Publication Model: Print Cited Medium: Internet ISSN: 1896-1851 (Electronic) Linking ISSN: 12302821 NLM ISO Abbreviation: Acta Parasitol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2019- : Cham : Springer International Publishing Original Publication: Warszawa : Witold Stefanski Institute Of Parasitology |
| مواضيع طبية MeSH: | Genotype*, Cryptosporidiosis/*epidemiology , Cryptosporidiosis/*parasitology , Cryptosporidium parvum/*classification , Cryptosporidium parvum/*isolation & purification , Zoonoses/*epidemiology , Zoonoses/*parasitology, Adolescent ; Animals ; Child ; Child, Preschool ; Coinfection/epidemiology ; Coinfection/parasitology ; Cross-Sectional Studies ; Cryptosporidium parvum/genetics ; DNA Fingerprinting ; DNA, Protozoan/genetics ; Diarrhea/epidemiology ; Diarrhea/parasitology ; Egypt/epidemiology ; Humans ; Infant ; Microscopy ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; Prevalence |
| مستخلص: | Cryptosporidium is a genus of zoonotic pathogens transmissible from a variety of animals to humans and is a considerable public health concern. It is a significant cause of diarrheal disease in developing and industrialized nations. Cryptosporidium parvum and Cryptosporidium hominis are the main agents of cryptosporidiosis in humans. In this study we identified the genotypes of the Cryptosporidium isolates from clinical samples from diarrheic children using polymerase chain reaction (PCR) amplification and restriction fragment length polymorphism (RFLP) analyses of the TRAP-C2 gene (Thrompodin Related Adhesive Protein). A total of 430 fecal specimens from 1 to 14 years children were collected from inpatient and outpatient clinics of Benha University, Educational and Children Specialized Hospitals, Benha, Qalubyia, and were microscopically examined for Cryptosporidium spp. All infected samples were also analyzed using nested PCR. A polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis of the (266-366 bp) of TRAP-C2 gene was also used to detect and identify Cryptosporidium spp. in PCR- positive samples. The results showed that 50 (11.63%) of the specimens were positive for Cryptosporidium spp. Genomic amplification and restriction digestion of the PCR products by BstETI, Hae III for TRAP-C2 gene restriction enzymes revealed that 82% (41/50) had C. parvum, 12% (6/50) had C. hominis, and three (3/50) samples (6%) had mixed infections. In conclusion, elevated prevalence of C. parvum, suggesting animal-human (zoonotic) transmission and further investigations are required to determine the subgenotypes of C. parvum to clarify the mode of transmission in order to improve the control measures. |
| المشرفين على المادة: | 0 (DNA, Protozoan) |
| تواريخ الأحداث: | Date Created: 20150724 Date Completed: 20160317 Latest Revision: 20190722 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1515/ap-2015-0004 |
| PMID: | 26204017 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1896-1851 |
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| DOI: | 10.1515/ap-2015-0004 |