دورية أكاديمية
Antibody-free ultra-high performance liquid chromatography/tandem mass spectrometry measurement of angiotensin I and II using magnetic epitope-imprinted polymers.
| العنوان: | Antibody-free ultra-high performance liquid chromatography/tandem mass spectrometry measurement of angiotensin I and II using magnetic epitope-imprinted polymers. |
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| المؤلفون: | Tan L; MOE Key Laboratory of Laser Life Science, South China Normal University, Guangzhou 510006, China; Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China., Yu Z; MOE Key Laboratory of Laser Life Science, South China Normal University, Guangzhou 510006, China., Zhou X; MOE Key Laboratory of Laser Life Science, South China Normal University, Guangzhou 510006, China., Xing D; MOE Key Laboratory of Laser Life Science, South China Normal University, Guangzhou 510006, China., Luo X; Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China., Peng R; Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China., Tang Y; MOE Key Laboratory of Laser Life Science, South China Normal University, Guangzhou 510006, China. Electronic address: tanglab@scnu.edu.cn. |
| المصدر: | Journal of chromatography. A [J Chromatogr A] 2015 Sep 11; Vol. 1411, pp. 69-76. Date of Electronic Publication: 2015 Aug 01. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9318488 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3778 (Electronic) Linking ISSN: 00219673 NLM ISO Abbreviation: J Chromatogr A Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam ; New York : Elsevier, 1993- |
| مواضيع طبية MeSH: | Magnetite Nanoparticles*, Acrylamides/*chemistry , Angiotensin I/*analysis , Angiotensin II/*analysis , Polymethacrylic Acids/*chemistry, Chromatography, High Pressure Liquid/methods ; Chromatography, Liquid/methods ; Epitopes ; Feasibility Studies ; Humans ; Molecular Imprinting ; Reproducibility of Results ; Sensitivity and Specificity ; Tandem Mass Spectrometry/methods |
| مستخلص: | The major challenges in measuring plasma renin activity (PRA) stem from the complexity of biological matrix, as well as from the instability and low circulating concentration of angiotensin. In this study, an ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) based technique has been developed for the measurement of angiotensin using magnetically imprinted polymers for simultaneous enrichment of the precursor peptide angiotensin II (Ang II) and the upstream peptide precursor angiotensin I (Ang I). This technique involved surface graft imprinting in aqueous solutions using vinyl-modified nano-iron oxide as solid supports, the specificity determinant of Ang I and Ang II as the epitope, and methacrylic acid and N-t-butylacrylamide as functional monomers. The vinyl-modified nano-iron oxide acted as a magnetic separation media, and the molecularly imprinted shell provided analyte selectivity for the recognition of Ang I and Ang II. Selective enrichment of Ang I and Ang II was accomplished by the magnetically imprinted polymers, followed by a magnetic separation procedure and subsequent quantification by UPLC-MS/MS in the positive ionization mode with multiple reaction monitoring. Through the latter protocol, a low limit of detection could be realized, viz. 0.07ng/mL and 0.06ng/mL for Ang I and II, respectively, which was thoroughly validated for accuracy and reproducibility through analyzing Ang I and Ang II in human plasma samples. (Copyright © 2015 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Angiotensin; Epitope; Magnetic separation; Molecular imprinting; UPLC–MS/MS |
| المشرفين على المادة: | 0 (Acrylamides) 0 (Epitopes) 0 (Magnetite Nanoparticles) 0 (Polymethacrylic Acids) 11128-99-7 (Angiotensin II) 9041-90-1 (Angiotensin I) XJ13FSH48K (tert-butylacrylamide) |
| تواريخ الأحداث: | Date Created: 20150811 Date Completed: 20151203 Latest Revision: 20181202 |
| رمز التحديث: | 20240513 |
| DOI: | 10.1016/j.chroma.2015.07.114 |
| PMID: | 26256923 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-3778 |
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| DOI: | 10.1016/j.chroma.2015.07.114 |