دورية أكاديمية
[SOI-nanowire biosensor for the detection of D-NFAT 1 protein].
| العنوان: | [SOI-nanowire biosensor for the detection of D-NFAT 1 protein]. |
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| المؤلفون: | Malsagova KA; Institute of Biomedical Chemistry, Moscow, Russia., Ivanov YD; Institute of Biomedical Chemistry, Moscow, Russia., Pleshakova TO; Institute of Biomedical Chemistry, Moscow, Russia., Kozlov AF; Institute of Biomedical Chemistry, Moscow, Russia., Krohin NV; Institute of Biomedical Chemistry, Moscow, Russia., Kaysheva AL; Institute of Biomedical Chemistry, Moscow, Russia., Shumov ID; Institute of Biomedical Chemistry, Moscow, Russia., Popov VP; Rzhanov Institute of Semiconductor Physics, Novosibirsk, Russia., Naumova OV; Rzhanov Institute of Semiconductor Physics, Novosibirsk, Russia., Fomin BI; Rzhanov Institute of Semiconductor Physics, Novosibirsk, Russia., Nasimov DA; Rzhanov Institute of Semiconductor Physics, Novosibirsk, Russia. |
| المصدر: | Biomeditsinskaia khimiia [Biomed Khim] 2015 Jul-Aug; Vol. 61 (4), pp. 462-7. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | Russian |
| بيانات الدورية: | Publisher: Rossiĭskai︠a︡ akademii︠a︡ medit︠s︡inskikh nauk Country of Publication: Russia (Federation) NLM ID: 101196966 Publication Model: Print Cited Medium: Print ISSN: 2310-6972 (Print) Linking ISSN: 23106905 NLM ISO Abbreviation: Biomed Khim Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Moskva : Rossiĭskai︠a︡ akademii︠a︡ medit︠s︡inskikh nauk, 2003- |
| مواضيع طبية MeSH: | Aptamers, Nucleotide/*chemistry , Biosensing Techniques/*instrumentation , NFATC Transcription Factors/*analysis , Nanowires/*chemistry , Neoplasm Proteins/*analysis, Antibodies, Monoclonal/chemistry ; Aptamers, Nucleotide/chemical synthesis ; Biosensing Techniques/economics ; Electrochemical Techniques ; Humans ; Limit of Detection ; Silicon Dioxide/chemistry ; Solutions ; Succinimides/chemistry |
| مستخلص: | The nanowire (NW) detection is one of fast-acting and high-sensitive methods allowing to reveal potentially relevant protein molecules. A NW biosensor based on the silicon-on-insulator (SOI)-structures was used for biospecific label-free detection of NFAT 1 (D-NFAT 1) oncomarker in real time. For this purpose, SOI-nanowires (NWs) were modified with aptamers against NFAT 1 used as molecular probes. It was shown that using this biosensor it is possible to reach the sensitivity of ~10(-15) M. This sensitivity was comparable with that of the NW biosensor with immobilized antibodies used as macromolecular probes. The results demonstrate promising approaches used to form the sensor elements for high-sensitive disease diagnostics. |
| فهرسة مساهمة: | Keywords: D-NFAT 1; aptamer; biosensor; nanowires; silicon-on-insulator Local Abstract: [Publisher, Russian] Odnim iz bystrodeĭstvuiushchikh i vysokochuvstvitel'nykh metodov, pozvoliaiushchikh vyiavliat' potentsial'no vazhnye belkovye molekuly, iavliaetsia metod nanoprovolochnoĭ (NP) detektsii. Nanoprovolochnyĭ biosensor na osnove struktur kremniĭ na izoliatore (KNI), byl ispol'zovan dlia biospetsificheskoĭ detektsii NFAT 1 (D-NFAT 1)-onkomarkera v real'nom vremeni bez metok. Dlia étogo KNI-nanoprovoloki byli modifitsirovany aptamerami k NFAT 1, ispol'zuemymi v kachestve makromolekuliarnykh zondov. Pokazano, chto takoĭ biosensor pozvoliaet dostigat' chuvstvitel'nost' poriadka 10-15 M. Éta chuvstvitel'nost' sopostavima s chuvstvitel'nost'iu, poluchennoĭ na nanoprovolochnom biosensore s immobilizovannymi antitelami, ispol'zuemymi v kachestve makromolekuliarnykh zondov. Rezul'taty demonstriruiut perspektivnost' ispol'zovannykh v rabote podkhodov formirovaniia sensornykh élementov dlia vysokochuvstvitel'noĭ diagnostiki zabolevaniĭ. |
| المشرفين على المادة: | 0 (Antibodies, Monoclonal) 0 (Aptamers, Nucleotide) 0 (NFATC Transcription Factors) 0 (NFATC2 protein, human) 0 (Neoplasm Proteins) 0 (Solutions) 0 (Succinimides) 7631-86-9 (Silicon Dioxide) EVY5D0U6SH (dithiobis(succinimidylpropionate)) |
| تواريخ الأحداث: | Date Created: 20150910 Date Completed: 20151124 Latest Revision: 20181023 |
| رمز التحديث: | 20240628 |
| DOI: | 10.18097/PBMC20156104462 |
| PMID: | 26350736 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2310-6972 |
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| DOI: | 10.18097/PBMC20156104462 |