دورية أكاديمية
Distribution and Abundance of Hopanoid Producers in Low-Oxygen Environments of the Eastern Pacific Ocean.
| العنوان: | Distribution and Abundance of Hopanoid Producers in Low-Oxygen Environments of the Eastern Pacific Ocean. |
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| المؤلفون: | Kharbush JJ; Scripps Institution of Oceanography, UCSD, 9500 Gilman Drive, La Jolla, CA, 92093, USA. jkharbus@ucsd.edu., Kejriwal K; Scripps Institution of Oceanography, UCSD, 9500 Gilman Drive, La Jolla, CA, 92093, USA., Aluwihare LI; Scripps Institution of Oceanography, UCSD, 9500 Gilman Drive, La Jolla, CA, 92093, USA. |
| المصدر: | Microbial ecology [Microb Ecol] 2016 Feb; Vol. 71 (2), pp. 401-8. Date of Electronic Publication: 2015 Sep 16. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer-Verlag Country of Publication: United States NLM ID: 7500663 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-184X (Electronic) Linking ISSN: 00953628 NLM ISO Abbreviation: Microb Ecol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York, Springer-Verlag. |
| مواضيع طبية MeSH: | Bacteria/*isolation & purification , Bacteria/*metabolism , Oxygen/*analysis , Seawater/*microbiology , Triterpenes/*metabolism, Bacteria/classification ; Bacteria/genetics ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Oxygen/metabolism ; Pacific Ocean ; Phylogeny ; Seawater/analysis |
| مستخلص: | Hopanoids are bacterial membrane lipid biomarker molecules that feature prominently in the molecular fossil record. In the modern marine water column, recent reports implicate bacteria inhabiting low-oxygen environments as important sources of hopanoids to marine sediments. However, the preliminary biogeography reported by recent studies and the environmental conditions governing such distributions can only be confirmed when the numerical abundance of these organisms is known with more certainty. In this study, we employ two different approaches to examine the quantitative significance of phylogenetically distinct hopanoid producers in low-oxygen environments. First, we develop a novel quantitative PCR (qPCR) assay for the squalene hopene cyclase (sqhC) gene, targeting a subset of hopanoid producers previously identified to be important in the eastern North Pacific Ocean. The results represent the first quantitative gene abundance data of any kind for hopanoid producers in the marine water column and show that these putative alphaproteobacterial hopanoid producers are rare, comprising at most 0.2 % of the total bacterial community in our samples. Second, a complementary analysis of existing low-oxygen metagenomic datasets further examined the generality of the qPCR observation. We find that the dominant sqhC sequences in these metagenomic datasets are associated with phyla such as Nitrospinae rather than Proteobacteria, consistent with the qPCR finding that alphaproteobacterial hopanoid producers are not very abundant in low-oxygen environments. In fact, positive correlations between sqhC gene abundance and environmental parameters in these samples identify nitrite availability as a potentially important factor in the ecology of hopanoid producers that dominate low-oxygen environments. |
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| فهرسة مساهمة: | Keywords: Lipid biomarkers; Metagenomics; Nitrite; Squalene hopene cyclase; qPCR |
| المشرفين على المادة: | 0 (Bacterial Proteins) 0 (Triterpenes) S88TT14065 (Oxygen) |
| تواريخ الأحداث: | Date Created: 20150918 Date Completed: 20161018 Latest Revision: 20181113 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1007/s00248-015-0671-y |
| PMID: | 26377203 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1432-184X |
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| DOI: | 10.1007/s00248-015-0671-y |