دورية أكاديمية
أعرض في EDS

The mitochondrial genome of Angiostrongylus mackerrasae as a basis for molecular, epidemiological and population genetic studies.

التفاصيل البيبلوغرافية
العنوان: The mitochondrial genome of Angiostrongylus mackerrasae as a basis for molecular, epidemiological and population genetic studies.
المؤلفون: Aghazadeh M; School of Veterinary Science, University of Queensland, Queensland, 4343, Australia. mahdis.aghazadeh@uqconnect.edu.au.; QIMR Berghofer Medical Research Institute, Brisbane, Queensland, 4006, Australia. mahdis.aghazadeh@uqconnect.edu.au., Traub RJ; Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Victoria, 3052, Australia., Mohandas N; Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Victoria, 3052, Australia., Aland KV; Queensland Museum and Sciencentre, Queensland, 4101, Australia., Reid SA; School of Public Health, University of Queensland, Queensland, 4006, Australia., McCarthy JS; QIMR Berghofer Medical Research Institute, Brisbane, Queensland, 4006, Australia.; School of Public Health, University of Queensland, Queensland, 4006, Australia., Jones MK; School of Veterinary Science, University of Queensland, Queensland, 4343, Australia.; QIMR Berghofer Medical Research Institute, Brisbane, Queensland, 4006, Australia.
المصدر: Parasites & vectors [Parasit Vectors] 2015 Sep 17; Vol. 8, pp. 473. Date of Electronic Publication: 2015 Sep 17.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101462774 Publication Model: Electronic Cited Medium: Internet ISSN: 1756-3305 (Electronic) Linking ISSN: 17563305 NLM ISO Abbreviation: Parasit Vectors Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central
مواضيع طبية MeSH: Genome, Mitochondrial* , Sequence Analysis, DNA*, Angiostrongylus/*genetics , DNA, Helminth/*chemistry , DNA, Helminth/*genetics, Angiostrongylus/isolation & purification ; Animals ; Australia ; Cluster Analysis ; Gene Order ; Phylogeny ; Rats ; Sequence Homology
مستخلص: Background: Angiostrongylus mackerrasae is a metastrongyloid nematode endemic to Australia, where it infects the native bush rat, Rattus fuscipes. This lungworm has an identical life cycle to that of Angiostrongylus cantonensis, a leading cause of eosinophilic meningitis in humans. The ability of A. mackerrasae to infect non-rodent hosts, specifically the black flying fox, raises concerns as to its zoonotic potential. To date, data on the taxonomy, epidemiology and population genetics of A. mackerrasae are unknown. Here, we describe the mitochondrial (mt) genome of A. mackerrasae with the aim of starting to address these knowledge gaps.
Methods: The complete mitochondrial (mt) genome of A. mackerrasae was amplified from a single morphologically identified adult worm, by long-PCR in two overlapping amplicons (8 kb and 10 kb). The amplicons were sequenced using the MiSeq Illumina platform and annotated using an in-house pipeline. Amino acid sequences inferred from individual protein coding genes of the mt genomes were concatenated and then subjected to phylogenetic analysis using Bayesian inference.
Results: The mt genome of A. mackerrasae is 13,640 bp in size and contains 12 protein coding genes (cox1-3, nad1-6, nad4L, atp6 and cob), and two ribosomal RNA (rRNA) and 22 transfer RNA (tRNA) genes.
Conclusions: The mt genome of A. mackerrasae has similar characteristics to those of other Angiostrongylus species. Sequence comparisons reveal that A. mackerrasae is closely related to A. cantonensis and the two sibling species may have recently diverged compared with all other species in the genus with a highly specific host selection. This mt genome will provide a source of genetic markers for explorations of the epidemiology, biology and population genetics of A. mackerrasae.
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المشرفين على المادة: 0 (DNA, Helminth)
تواريخ الأحداث: Date Created: 20150919 Date Completed: 20160622 Latest Revision: 20181113
رمز التحديث: 20240829
مُعرف محوري في PubMed: PMC4574185
DOI: 10.1186/s13071-015-1082-0
PMID: 26381738
قاعدة البيانات: MEDLINE
الوصف
تدمد:1756-3305
DOI:10.1186/s13071-015-1082-0