دورية أكاديمية
Testosterone and estradiol treatments differently affect pituitary-thyroid axis and liver deiodinase 1 activity in orchidectomized middle-aged rats.
| العنوان: | Testosterone and estradiol treatments differently affect pituitary-thyroid axis and liver deiodinase 1 activity in orchidectomized middle-aged rats. |
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| المؤلفون: | Šošić-Jurjević B; Institute for Biological Research 'Siniša Stanković', University of Belgrade, Despot Stefan Blvd. 142, 11000 Belgrade, Serbia. Electronic address: brankasj@ibiss.bg.ac.rs., Filipović B; Institute for Biological Research 'Siniša Stanković', University of Belgrade, Despot Stefan Blvd. 142, 11000 Belgrade, Serbia., Renko K; Institut für Experimentelle Endokrinologie, Charité - Universitätsmedizin Berlin, Augustenburger Platz 1, D-13353 Berlin, Germany., Miler M; Institute for Biological Research 'Siniša Stanković', University of Belgrade, Despot Stefan Blvd. 142, 11000 Belgrade, Serbia., Trifunović S; Institute for Biological Research 'Siniša Stanković', University of Belgrade, Despot Stefan Blvd. 142, 11000 Belgrade, Serbia., Ajdžanovič V; Institute for Biological Research 'Siniša Stanković', University of Belgrade, Despot Stefan Blvd. 142, 11000 Belgrade, Serbia., Kӧhrle J; Institut für Experimentelle Endokrinologie, Charité - Universitätsmedizin Berlin, Augustenburger Platz 1, D-13353 Berlin, Germany., Milošević V; Institute for Biological Research 'Siniša Stanković', University of Belgrade, Despot Stefan Blvd. 142, 11000 Belgrade, Serbia. |
| المصدر: | Experimental gerontology [Exp Gerontol] 2015 Dec; Vol. 72, pp. 85-98. Date of Electronic Publication: 2015 Sep 15. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: England NLM ID: 0047061 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-6815 (Electronic) Linking ISSN: 05315565 NLM ISO Abbreviation: Exp Gerontol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Tarrytown Ny : Elsevier Science Original Publication: Oxford. |
| مواضيع طبية MeSH: | Aging*, Estradiol/*analogs & derivatives , Iodide Peroxidase/*metabolism , Pituitary Gland/*pathology , Testosterone/*administration & dosage , Thyroid Gland/*pathology , Thyrotropin/*blood, Animals ; Body Weight ; Estradiol/administration & dosage ; Immunohistochemistry ; Liver/drug effects ; Liver/enzymology ; Male ; Orchiectomy ; Rats ; Rats, Wistar ; Thyroxine/blood |
| مستخلص: | We previously reported that orchidectomy (Orx) of middle-aged rats (15-16-month-old; MA) slightly affected pituitary-thyroid axis, but decreased liver deiodinase (Dio) type 1 and pituitary Dio2 enzyme activities. At present, we examined the effects of subsequent testosterone-propionate treatment (5mg/kg; Orx+T), and compared the effects of testosterone with the effects of estradiol-dipropionate (0.06mg/kg; Orx+E) treatment. Hormones were subcutaneously administered, daily, for three weeks, while Orx and sham-operated (SO) controls received only the vehicle. The applied dose of T did not alter serum TSH, T4 and T3 concentrations in Orx- MA, though it increased TSH when administrated to Orx young adults (2.5-month-old; Orx-YA). However, pituitaries of Orx-MA+T rats had higher relative intensity of immunofluorescence (RIF) for TSHβ; in their thyroids we found increased volume and height of follicular epithelium, decreased volume of the colloid and higher RIF for T4-bound to thyroglobulin (Tg-T4). Liver Dio1 activity was increased. E-treatment did not affect serum hormone levels, pituitary RIF for TSHβ, or liver Dio1 activity in Orx-MA rats. Thyroids had decreased relative volume and height of follicular epithelium, increased relative volume of the colloid, decreased volume of sodium-iodide symporter-immunopositive epithelium and lower RIF for Tg-T4. Detected changes were statistically significant. In conclusion, androgenization enhanced pituitary TSHβ RIF, thyroid activation and liver Dio1 enzyme activity in Orx-MA, without elevating serum TSH as in Orx-YA rats. Estrogenization induced pituitary enlargement with no effect on pituitary TSHβ RIF, serum TSH or liver Dio1 activity. E also induced alterations in thyroid histology that indicate mild suppression of its functioning, and contributed to thyroid blood vessel enlargement in Orx-MA rats. (Copyright © 2015 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Estradiol; Middle age; Pituitary-thyroid axis; Rat; Testosterone |
| المشرفين على المادة: | 3XMK78S47O (Testosterone) 4TI98Z838E (Estradiol) 9002-71-5 (Thyrotropin) EC 1.11.1.- (iodothyronine deiodinase type I) EC 1.11.1.8 (Iodide Peroxidase) NIG5418BXB (estradiol dipropionate) Q51BO43MG4 (Thyroxine) |
| تواريخ الأحداث: | Date Created: 20150919 Date Completed: 20160908 Latest Revision: 20151120 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.exger.2015.09.010 |
| PMID: | 26384168 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-6815 |
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| DOI: | 10.1016/j.exger.2015.09.010 |