دورية أكاديمية
أعرض في EDS

The antimicrobial activity of free and immobilized poly (diallyldimethylammonium) chloride in nanoparticles of poly (methylmethacrylate).

التفاصيل البيبلوغرافية
العنوان: The antimicrobial activity of free and immobilized poly (diallyldimethylammonium) chloride in nanoparticles of poly (methylmethacrylate).
المؤلفون: Sanches LM; Biocolloids Lab, Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Caixa Postal 26077, CEP 05513-970, São Paulo, SP, Brazil. luccas.sanches@hotmail.com., Petri DF; Instituto de Química, Universidade de São Paulo, Caixa Postal 26077, CEP 05513-970, São Paulo, SP, Brazil. dfsp@usp.br., de Melo Carrasco LD; Biocolloids Lab, Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Caixa Postal 26077, CEP 05513-970, São Paulo, SP, Brazil. lemelodias@usp.br.; Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, CEP 05508-900, São Paulo, Brazil. lemelodias@usp.br., Carmona-Ribeiro AM; Biocolloids Lab, Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Caixa Postal 26077, CEP 05513-970, São Paulo, SP, Brazil. amcr@usp.br.; Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, CEP 05508-900, São Paulo, Brazil. amcr@usp.br.
المصدر: Journal of nanobiotechnology [J Nanobiotechnology] 2015 Sep 24; Vol. 13, pp. 58. Date of Electronic Publication: 2015 Sep 24.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101152208 Publication Model: Electronic Cited Medium: Internet ISSN: 1477-3155 (Electronic) Linking ISSN: 14773155 NLM ISO Abbreviation: J Nanobiotechnology Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, 2003-
مواضيع طبية MeSH: Allyl Compounds/*administration & dosage , Allyl Compounds/*pharmacology , Anti-Infective Agents/*administration & dosage , Anti-Infective Agents/*pharmacology , Drug Carriers/*chemistry , Nanoparticles/*chemistry , Polymethyl Methacrylate/*chemistry , Quaternary Ammonium Compounds/*administration & dosage , Quaternary Ammonium Compounds/*pharmacology, Allyl Compounds/chemistry ; Anti-Infective Agents/chemistry ; Candida albicans/drug effects ; Candidiasis/drug therapy ; Escherichia coli/drug effects ; Escherichia coli Infections/drug therapy ; Humans ; Microbial Sensitivity Tests ; Nanoparticles/ultrastructure ; Quaternary Ammonium Compounds/chemistry ; Staphylococcal Infections/drug therapy ; Staphylococcus aureus/drug effects
مستخلص: Background: Several cationic polymers exhibit a useful antimicrobial property, however the structure-activity relationship still requires a more complete investigation. The main objective of this work is the comparison between the antimicrobial activity and toxicity of free and immobilized poly (diallyldimethylammonium) chloride (PDDA) in biocompatible poly (methylmethacrylate) (PMMA) nanoparticles (NPs).
Results: NPs synthesis by emulsion polymerization is performed over a range of [PDDA] at two methylmethacrylate (MMA) concentrations. The PMMA/PDDA dispersions are characterized by dynamic light-scattering for sizing, polydispersity and zeta-potential analysis, scanning electron microscopy (SEM), plating plus colony forming unities (CFU) counting for determination of the minimal microbicidal concentrations (MMC) against Escherichia coli, Staphylococcus aureus and Candida albicans and hemolysis evaluation against mammalian erythrocytes. There is a high colloidal stability for the cationic PMMA/PDDA NPs over a range of [PDDA]. NPs diverse antimicrobial activity against the microorganisms reduces cell viability by eight-logs (E. coli), seven-logs (S. aureus) or two-logs (C. albicans). The NPs completely kill E. coli over a range of [PDDA] that are innocuous to the erythrocytes. Free PDDA antimicrobial activity is higher than the one observed for PDDA in the NPs. There is no PDDA induced-hemolysis at the MMC in contrast to the hemolytic effect of immobilized PDDA in the NPs. Hemolysis is higher than 15 % for immobilized PDDA at the MMC for S. aureus and C. albicans.
Conclusions: The mobility of the cationic antimicrobial polymer PDDA determines its access to the inner layers of the cell wall and the cell membrane, the major sites of PDDA antimicrobial action. PDDA freedom does matter for determining the antimicrobial activity at low PDDA concentrations and absence of hemolysis.
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المشرفين على المادة: 0 (Allyl Compounds)
0 (Anti-Infective Agents)
0 (Drug Carriers)
0 (Quaternary Ammonium Compounds)
8MC08B895B (diallyldimethylammonium chloride)
9011-14-7 (Polymethyl Methacrylate)
تواريخ الأحداث: Date Created: 20150926 Date Completed: 20160315 Latest Revision: 20190107
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC4582890
DOI: 10.1186/s12951-015-0123-3
PMID: 26404400
قاعدة البيانات: MEDLINE
الوصف
تدمد:1477-3155
DOI:10.1186/s12951-015-0123-3