دورية أكاديمية
أعرض في EDS

Inactivation of the Autolysis-Related Genes lrgB and yycI in Staphylococcus aureus Increases Cell Lysis-Dependent eDNA Release and Enhances Biofilm Development In Vitro and In Vivo.

التفاصيل البيبلوغرافية
العنوان: Inactivation of the Autolysis-Related Genes lrgB and yycI in Staphylococcus aureus Increases Cell Lysis-Dependent eDNA Release and Enhances Biofilm Development In Vitro and In Vivo.
المؤلفون: Beltrame CO; Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, Departamento de Microbiologia Médica, Rio de Janeiro, RJ, Brazil., Côrtes MF; Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, Departamento de Microbiologia Médica, Rio de Janeiro, RJ, Brazil., Bonelli RR; Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, Departamento de Microbiologia Médica, Rio de Janeiro, RJ, Brazil., Côrrea AB; Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, Departamento de Microbiologia Médica, Rio de Janeiro, RJ, Brazil., Botelho AM; Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, Departamento de Microbiologia Médica, Rio de Janeiro, RJ, Brazil., Américo MA; Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, Departamento de Microbiologia Médica, Rio de Janeiro, RJ, Brazil., Fracalanzza SE; Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, Departamento de Microbiologia Médica, Rio de Janeiro, RJ, Brazil., Figueiredo AM; Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, Departamento de Microbiologia Médica, Rio de Janeiro, RJ, Brazil.
المصدر: PloS one [PLoS One] 2015 Sep 25; Vol. 10 (9), pp. e0138924. Date of Electronic Publication: 2015 Sep 25 (Print Publication: 2015).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Bacterial Proteins/*genetics , Biofilms/*growth & development , DNA, Bacterial/*metabolism , Staphylococcal Infections/*microbiology , Staphylococcus aureus/*physiology , Virulence Factors/*genetics, Animals ; Bacteriolysis ; Disease Models, Animal ; Gene Expression Regulation, Bacterial ; Gene Library ; Humans ; In Vitro Techniques ; Mice ; Mutagenesis, Insertional ; Staphylococcal Infections/genetics ; Staphylococcus aureus/genetics ; Staphylococcus aureus/isolation & purification
مستخلص: Staphylococcus aureus ica-independent biofilms are multifactorial in nature, and various bacterial proteins have been associated with biofilm development, including fibronectin-binding proteins A and B, protein A, surface protein SasG, proteases, and some autolysins. The role of extracellular DNA (eDNA) has also been demonstrated in some S. aureus biofilms. Here, we constructed a Tn551 library, and the screening identified two genes that affected biofilm formation, lrgB and yycI. The repressive effect of both genes on the development of biofilm was also confirmed in knockout strains constructed by allelic recombination. In contrast, the superexpression of either lrgB or yycI by a cadmium-inducible promoter led to a decrease in biofilm accumulation. Indeed, a significant increase in the cell-lysis dependent eDNA release was detected when lrgB or yycI were inactivated, explaining the enhanced biofilm formed by these mutants. In fact, lrgB and yycI genes belong to distinct operons that repress bacterial autolysis through very different mechanisms. LrgB is associated with the synthesis of phage holin/anti-holin analogues, while YycI participates in the activation/repression of the two-component system YycGF (WalKR). Our in vivo data suggest that autolysins activation lead to increased bacterial virulence in the foreign body animal model since a higher number of attached cells was recovered from the implanted catheters inoculated with lrgB or yycI knockout mutants.
References: J Bacteriol. 2007 Apr;189(8):3290-5. (PMID: 17307848)
MBio. 2012;3(2). pii: e00277-11. doi: 10.1128/mBio.00277-11. (PMID: 22434850)
Biomaterials. 2012 Sep;33(26):5967-82. (PMID: 22695065)
PLoS One. 2012;7(7):e42244. (PMID: 22860095)
Mol Microbiol. 2012 Dec;86(5):1183-96. (PMID: 23075270)
BMC Microbiol. 2013;13:93. (PMID: 23622558)
Res Microbiol. 2013 Sep;164(7):695-700. (PMID: 23774059)
PLoS One. 2013;8(9):e77122. (PMID: 24098817)
Nat Rev Microbiol. 2014 Jan;12(1):63-9. (PMID: 24336185)
PLoS One. 2013;8(12):e83743. (PMID: 24367612)
PLoS One. 2014;9(3):e91935. (PMID: 24651318)
Paediatr Int Child Health. 2014 May;34(2):108-14. (PMID: 24621234)
Int J Med Microbiol. 2015 Jan;305(1):140-7. (PMID: 25547264)
Trends Microbiol. 2000 Mar;8(3):120-8. (PMID: 10707065)
J Bacteriol. 2000 Apr;182(7):1794-801. (PMID: 10714982)
J Bacteriol. 2001 May;183(9):2888-96. (PMID: 11292810)
Science. 2002 Feb 22;295(5559):1487. (PMID: 11859186)
PLoS One. 2009;4(6):e5822. (PMID: 19513119)
J Bacteriol. 2009 Feb;191(3):832-43. (PMID: 19047354)
J Bacteriol. 2003 Apr;185(8):2635-43. (PMID: 12670989)
J Bacteriol. 2009 Aug;191(15):4767-75. (PMID: 19502411)
Infect Immun. 2009 Sep;77(9):3978-91. (PMID: 19581398)
Proc Natl Acad Sci U S A. 2010 Feb 2;107(5):2219-23. (PMID: 20080651)
Mol Microbiol. 2003 Jun;48(6):1451-66. (PMID: 12791130)
Infect Immun. 2003 Jul;71(7):4206-11. (PMID: 12819120)
J Bacteriol. 2004 Aug;186(16):5267-80. (PMID: 15292128)
Appl Environ Microbiol. 2004 Oct;70(10):6076-85. (PMID: 15466553)
J Bacteriol. 1986 Apr;166(1):29-33. (PMID: 3957869)
Eur J Clin Microbiol. 1986 Dec;5(6):714-8. (PMID: 3026802)
Antimicrob Agents Chemother. 1990 Jan;34(1):33-9. (PMID: 1691615)
DNA Seq. 1992;2(5):281-7. (PMID: 1633325)
J Bacteriol. 1993 Mar;175(5):1493-9. (PMID: 8095258)
EMBO J. 1993 Oct;12(10):3967-75. (PMID: 7691599)
Antimicrob Agents Chemother. 1994 Nov;38(11):2590-8. (PMID: 7872753)
FEMS Microbiol Rev. 1995 Aug;17(1-2):191-205. (PMID: 7669346)
J Clin Microbiol. 1999 Mar;37(3):504-9. (PMID: 9986803)
J Bacteriol. 2005 Jun;187(12):4064-76. (PMID: 15937169)
J Infect Dis. 2005 Sep 1;192(5):801-10. (PMID: 16088829)
J Clin Microbiol. 2007 May;45(5):1379-88. (PMID: 17329452)
Proc Natl Acad Sci U S A. 2007 May 8;104(19):8113-8. (PMID: 17452642)
Microbiology. 2007 Aug;153(Pt 8):2435-46. (PMID: 17660408)
J Bacteriol. 2007 Nov;189(22):8257-69. (PMID: 17827301)
Microbiol Mol Biol Rev. 2008 Mar;72(1):85-109, table of contents. (PMID: 18322035)
Curr Top Microbiol Immunol. 2008;322:207-28. (PMID: 18453278)
J Bacteriol. 2008 Jun;190(11):3835-50. (PMID: 18375547)
Mol Microbiol. 2008 Aug;69(4):784-793. (PMID: 18788120)
Adv Exp Med Biol. 2008;631:214-28. (PMID: 18792692)
Microbiology. 2008 Nov;154(Pt 11):3480-90. (PMID: 18957601)
BMC Microbiol. 2008;8:173. (PMID: 18842140)
Appl Environ Microbiol. 2010 Apr;76(7):2271-9. (PMID: 20139319)
PLoS One. 2010;5(4):e10146. (PMID: 20418950)
Proc Natl Acad Sci U S A. 2010 Aug 10;107(32):14407-12. (PMID: 20660751)
Nat Rev Microbiol. 2010 Sep;8(9):623-33. (PMID: 20676145)
Infect Immun. 2011 Mar;79(3):1153-65. (PMID: 21189325)
J Bacteriol. 2011 May;193(10):2468-76. (PMID: 21421752)
New Phytol. 2012 Jan;193(1):81-95. (PMID: 21916894)
Plant Cell Physiol. 2012 Jan;53(1):125-34. (PMID: 22180599)
J Microbiol Methods. 2012 Mar;88(3):393-8. (PMID: 22296887)
PLoS One. 2012;7(6):e38453. (PMID: 22685571)
J Med Microbiol. 2007 May;56(Pt 5):614-9. (PMID: 17446283)
المشرفين على المادة: 0 (Bacterial Proteins)
0 (DNA, Bacterial)
0 (Virulence Factors)
تواريخ الأحداث: Date Created: 20150926 Date Completed: 20160527 Latest Revision: 20181113
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC4583396
DOI: 10.1371/journal.pone.0138924
PMID: 26406329
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0138924