دورية أكاديمية
أعرض في EDS

Humans differ in their personal microbial cloud.

التفاصيل البيبلوغرافية
العنوان: Humans differ in their personal microbial cloud.
المؤلفون: Meadow JF; Biology and the Built Environment Center, University of Oregon , Eugene, OR , USA ; Department of Biology, Institute of Ecology and Evolution, University of Oregon , Eugene, OR , USA., Altrichter AE; Biology and the Built Environment Center, University of Oregon , Eugene, OR , USA ; Department of Biology, Institute of Ecology and Evolution, University of Oregon , Eugene, OR , USA., Bateman AC; Biology and the Built Environment Center, University of Oregon , Eugene, OR , USA ; Department of Biology, Institute of Ecology and Evolution, University of Oregon , Eugene, OR , USA., Stenson J; Biology and the Built Environment Center, University of Oregon , Eugene, OR , USA ; Department of Architecture, Energy Studies in Buildings Laboratory, University of Oregon , Eugene, OR , USA., Brown GZ; Biology and the Built Environment Center, University of Oregon , Eugene, OR , USA ; Department of Architecture, Energy Studies in Buildings Laboratory, University of Oregon , Eugene, OR , USA., Green JL; Biology and the Built Environment Center, University of Oregon , Eugene, OR , USA ; Department of Biology, Institute of Ecology and Evolution, University of Oregon , Eugene, OR , USA ; Santa Fe Institute , Santa Fe, NM , USA., Bohannan BJ; Biology and the Built Environment Center, University of Oregon , Eugene, OR , USA ; Department of Biology, Institute of Ecology and Evolution, University of Oregon , Eugene, OR , USA.
المصدر: PeerJ [PeerJ] 2015 Sep 22; Vol. 3, pp. e1258. Date of Electronic Publication: 2015 Sep 22 (Print Publication: 2015).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: PeerJ Inc Country of Publication: United States NLM ID: 101603425 Publication Model: eCollection Cited Medium: Print ISSN: 2167-8359 (Print) Linking ISSN: 21678359 NLM ISO Abbreviation: PeerJ Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Corte Madera, CA : PeerJ Inc.
مستخلص: Dispersal of microbes between humans and the built environment can occur through direct contact with surfaces or through airborne release; the latter mechanism remains poorly understood. Humans emit upwards of 10(6) biological particles per hour, and have long been known to transmit pathogens to other individuals and to indoor surfaces. However it has not previously been demonstrated that humans emit a detectible microbial cloud into surrounding indoor air, nor whether such clouds are sufficiently differentiated to allow the identification of individual occupants. We used high-throughput sequencing of 16S rRNA genes to characterize the airborne bacterial contribution of a single person sitting in a sanitized custom experimental climate chamber. We compared that to air sampled in an adjacent, identical, unoccupied chamber, as well as to supply and exhaust air sources. Additionally, we assessed microbial communities in settled particles surrounding each occupant, to investigate the potential long-term fate of airborne microbial emissions. Most occupants could be clearly detected by their airborne bacterial emissions, as well as their contribution to settled particles, within 1.5-4 h. Bacterial clouds from the occupants were statistically distinct, allowing the identification of some individual occupants. Our results confirm that an occupied space is microbially distinct from an unoccupied one, and demonstrate for the first time that individuals release their own personalized microbial cloud.
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فهرسة مساهمة: Keywords: Built environment; Human microbiome; Indoor air; Indoor microbiology; Microbial cloud
تواريخ الأحداث: Date Created: 20150930 Date Completed: 20150929 Latest Revision: 20220330
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC4582947
DOI: 10.7717/peerj.1258
PMID: 26417541
قاعدة البيانات: MEDLINE
الوصف
تدمد:2167-8359
DOI:10.7717/peerj.1258